scholarly journals Unraveling Assemblage, Functions and Stability of the Gut Microbiota of Blattella germanica by Antibiotic Treatment

2020 ◽  
Vol 11 ◽  
Author(s):  
Rebeca Domínguez-Santos ◽  
Ana Elena Pérez-Cobas ◽  
Alejandro Artacho ◽  
José A. Castro ◽  
Irene Talón ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Antibiotic Treatment ◽  
Blattella Germanica

scholarly journals Gut Microbiota Cannot Compensate the Impact of (quasi) Aposymbiosis in Blattella germanica

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1013
Author(s):  
Maria Muñoz-Benavent ◽  
Amparo Latorre ◽  
Ester Alemany-Cosme ◽  
Jesús Marín-Miret ◽  
Rebeca Domínguez-Santos ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Antibiotic Treatment ◽  
Adult Stage ◽  
Blattella Germanica ◽  
Gut Bacteria ◽  
Symbiotic System ◽  
The Impact

Blattella germanica presents a very complex symbiotic system, involving the following two kinds of symbionts: the endosymbiont Blattabacterium and the gut microbiota. Although the role of the endosymbiont has been fully elucidated, the function of the gut microbiota remains unclear. The study of the gut microbiota will benefit from the availability of insects deprived of Blattabacterium. Our goal is to determine the effect of the removal (or, at least, the reduction) of the endosymbiont population on the cockroach’s fitness, in a normal gut microbiota community. For this purpose, we treated our cockroach population, over several generations, with rifampicin, an antibiotic that only affects the endosymbiont during its extracellular phase, and decreases its amount in the following generation. As rifampicin also affects gut bacteria that are sensitive to this antibiotic, the treatment was performed during the first 12 days of the adult stage, which is the period when the endosymbiont infects the oocytes and lacks bacteriocyte protection. We found that after this antibiotic treatment, the endosymbiont population remained extremely reduced and only the microbiota was able to recover, although it could not compensate for the endosymbiont role, and the host’s fitness was drastically affected. This accomplished reduction, however, is not homogenous and requires further study to develop stable quasi-aposymbiotic cockroaches.

Contributions of Lactobacillus plantarum PC170 administration on the recovery of gut microbiota after short-term ceftriaxone exposure in mice

2020 ◽  
Vol 11 (5) ◽  
pp. 489-509
Author(s):  
R. Cheng ◽  
H. Liang ◽  
Y. Zhang ◽  
J. Guo ◽  
Z. Miao ◽  
...  
Keyword(s):  
Body Weight ◽  
Gut Microbiota ◽  
Lactobacillus Plantarum ◽  
Antibiotic Treatment ◽  
Recovery Phase ◽  
The Body ◽  
Faecal Microbiota ◽  
Short Term ◽  
Host Animal ◽  
The Impact

This study aimed to determine the impact of Lactobacillus plantarum PC170 concurrent with antibiotic treatment and/or during the recovery phase after antibiotic treatment on the body weight, faecal bacterial composition, short-chain fatty acids (SCFAs) concentration, and splenic cytokine mRNA expression of mice. Orally administrated ceftriaxone quantitatively and significantly decreased body weight, faecal total bacteria, Akkermansia muciniphila, and Lactobacillus plantarum, and faecal SCFAs concentration. Ceftriaxone treatment also dramatically altered the faecal microbiota with an increased Chao1 index, decreased species diversities and Bacteroidetes, and more Firmicutes and Proteobacteria. After ceftriaxone intervention, these changes all gradually started to recover. However, faecal microbiota diversities were still totally different from control by significantly increased α- and β-diversities. Bacteroidetes all flourished and became dominant during the recovery process. However, mice treated with PC170 both in parallel with and after ceftriaxone treatment encouraged more Bacteroidetes, Verrucomicrobia, and Actinobacteria, and the diversity by which to make faecal microbiota was very much closer to control. Furthermore, the expression of splenic pro-inflammatory cytokine tumour necrosis factor-α mRNA in mice supplemented with PC170 during the recovery phase was significantly lower than natural recovery. These results indicated that antibiotics, such as ceftriaxone, even with short-term intervention, could dramatically damage the structure of gut microbiota and their abilities to produce SCFAs with loss of body weight. Although such damages could be partly recovered with the cessation of antibiotics, the implication of antibiotics to gut microbiota might remain even after antibiotic treatment. The selected strain PC170 might be a potential probiotic because of its contributions in helping the host animal to remodel or stabilise its gut microbiome and enhancing the anti-inflammatory response as protection from the side effects of antibiotic therapy when it was administered in parallel with and after antibiotic treatment.

scholarly journals Oral iron supplementation after antibiotic exposure induces a deleterious recovery of the gut microbiota

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Thibault Cuisiniere ◽  
Annie Calvé ◽  
Gabriela Fragoso ◽  
Manon Oliero ◽  
Roy Hajjar ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Antibiotic Treatment ◽  
Iron Supplementation ◽  
Pentose Phosphate ◽  
Oral Iron ◽  
Oral Antibiotic ◽  
Antibiotic Exposure ◽  
Microbiota Composition ◽  
Fecal Samples ◽  
Excess Iron

Abstract Background Oral iron supplementation is commonly prescribed for anemia and may play an important role in the gut microbiota recovery of anemic individuals who received antibiotic treatment. This study aims to investigate the effects of iron supplementation on gut microbiota recovery after antibiotics exposure. Results Mice were subjected to oral antibiotic treatment with neomycin and metronidazole and were fed diets with different concentrations of iron. The composition of the gut microbiota was followed throughout treatment by 16S rRNA sequencing of DNA extracted from fecal samples. Gut microbiota functions were inferred using PICRUSt2, and short-chain fatty acid concentration in fecal samples was assessed by liquid-chromatography mass spectrometry. Iron supplementation after antibiotic exposure shifted the gut microbiota composition towards a Bacteroidetes phylum-dominant composition. At the genus level, the iron-supplemented diet induced an increase in the abundance of Parasutterella and Bacteroides, and a decrease of Bilophila and Akkermansia. Parasutterella excrementihominis, Bacteroides vulgatus, and Alistipes finegoldii, were more abundant with the iron excess diet. Iron-induced shifts in microbiota composition were accompanied by functional modifications, including an enhancement of the biosynthesis of primary bile acids, nitrogen metabolism, cyanoamino acid metabolism and pentose phosphate pathways. Recovery after antibiotic treatment increased propionate levels independent of luminal iron levels, whereas butyrate levels were diminished by excess iron. Conclusions Oral iron supplementation after antibiotic therapy in mice may lead to deleterious changes in the recovery of the gut microbiota. Our results have implications on the use of oral iron supplementation after antibiotic exposure and justify further studies on alternative treatments for anemia in these settings.

scholarly journals H. pylori eradication with antibiotic treatment causes changes in glucose homeostasis related to modifications in the gut microbiota

PLoS ONE ◽  
2019 ◽  
Vol 14 (3) ◽  
pp. e0213548 ◽  
Author(s):  
Gracia Mª Martín-Núñez ◽  
Isabel Cornejo-Pareja ◽  
Leticia Coin-Aragüez ◽  
Mª del Mar Roca-Rodríguez ◽  
Araceli Muñoz-Garach ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Antibiotic Treatment ◽  
Glucose Homeostasis ◽  
H Pylori

Effects of Antibiotic Treatment on Gut Microbiota and How to Overcome Its Negative Impacts on Human Health

2020 ◽  
Vol 6 (10) ◽  
pp. 2544-2559 ◽  
Author(s):  
Camila Fontoura Acosta Ribeiro ◽  
Gislaine Greice de Oliveira Silva Silveira ◽  
Elizabete de Souza Cândido ◽  
Marlon Henrique Cardoso ◽  
Cristiano Marcelo Espínola Carvalho ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Human Health ◽  
Antibiotic Treatment ◽  
Negative Impacts

scholarly journals Antepartum Antibiotic Treatment Increases Offspring Susceptibility to Experimental Colitis: A Role of the Gut Microbiota

PLoS ONE ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. e0142536 ◽  
Author(s):  
Peris Mumbi Munyaka ◽  
N. Eissa ◽  
Charles Noah Bernstein ◽  
Ehsan Khafipour ◽  
Jean-Eric Ghia
Keyword(s):  
Gut Microbiota ◽  
Antibiotic Treatment ◽  
Experimental Colitis

scholarly journals High-Throughput Sequencing Reveals the Incomplete, Short-Term Recovery of Infant Gut Microbiota following Parenteral Antibiotic Treatment with Ampicillin and Gentamicin

2012 ◽  
Vol 56 (11) ◽  
pp. 5811-5820 ◽  
Author(s):  
Fiona Fouhy ◽  
Caitriona M. Guinane ◽  
Seamus Hussey ◽  
Rebecca Wall ◽  
C. Anthony Ryan ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Antibiotic Treatment ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Short Term ◽  
Parenteral Antibiotic ◽  
Content Type ◽  
Combined Use ◽  
Cessation Of Treatment

ABSTRACTThe infant gut microbiota undergoes dramatic changes during the first 2 years of life. The acquisition and development of this population can be influenced by numerous factors, and antibiotic treatment has been suggested as one of the most significant. Despite this, however, there have been relatively few studies which have investigated the short-term recovery of the infant gut microbiota following antibiotic treatment. The aim of this study was to use high-throughput sequencing (employing both 16S rRNA andrpoB-specific primers) and quantitative PCR to compare the gut microbiota of nine infants who underwent parenteral antibiotic treatment with ampicillin and gentamicin (within 48 h of birth), 4 and 8 weeks after the conclusion of treatment, relative to that of nine matched healthy controls. The investigation revealed that the gut microbiota of the antibiotic-treated infants had significantly higher proportions ofProteobacteria(P= 0.0049) and significantly lower proportions ofActinobacteria(P= 0.00001) (and the associated genusBifidobacterium[P= 0.0132]) as well as the genusLactobacillus(P= 0.0182) than the untreated controls 4 weeks after the cessation of treatment. By week 8, theProteobacterialevels remained significantly higher in the treated infants (P= 0.0049), but theActinobacteria,Bifidobacterium, andLactobacilluslevels had recovered and were similar to those in the control samples. Despite this recovery of totalBifidobacteriumnumbers,rpoB-targeted pyrosequencing revealed that the number of differentBifidobacteriumspecies present in the antibiotic-treated infants was reduced. It is thus apparent that the combined use of ampicillin and gentamicin in early life can have significant effects on the evolution of the infant gut microbiota, the long-term health implications of which remain unknown.

scholarly journals Gut microbiota of the pine weevil degrades conifer diterpenes and increases insect fitness

2017 ◽  
Author(s):  
Aileen Berasategui ◽  
Hassan Salem ◽  
Christian Paetz ◽  
Maricel Santoro ◽  
Jonathan Gershenzon ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Gut Microbiota ◽  
Norway Spruce ◽  
Antibiotic Treatment ◽  
Hatching Rate ◽  
Pine Weevil ◽  
Pine Weevils ◽  
Gut Symbionts ◽  
Major Pest ◽  
Insect Fitness

AbstractThe pine weevil (Hylobius abietis), a major pest of conifer forests throughout Europe, feeds on the bark and cambium, tissues rich in terpenoid resins that are toxic to many insect herbivores. Here we report the ability of the pine weevil gut microbiota to degrade the diterpene acids of Norway spruce. The diterpene acid levels present in ingested bark were substantially reduced on passage through the pine weevil gut. This reduction was significantly less upon antibiotic treatment, and supplementing the diet with gut suspensions from untreated insects restored the ability to degrade diterpenes. In addition, cultured bacteria isolated from pine weevil guts were shown to degrade a Norway spruce diterpene acid. In a metagenomic survey of the insect’s bacterial community, we were able to annotate several genes of a previously described diterpene degradation (dit) gene cluster. Antibiotic treatment disrupted the core bacterial community of H. abietis guts and eliminated nearly all dit-genes concordant with its reduction of diterpene degradation. Pine weevils reared on an artificial diet spiked with diterpenes, but without antibiotics, were found to lay more eggs with a higher hatching rate than weevils raised on diets with antibiotics or without diterpenes. These results suggest that gut symbionts contribute towards host fitness, but not by detoxification of diterpenes, since these compounds do not show toxic effects with or without antibiotics. Rather the ability to thrive in a terpene rich environment appears to allow gut microbes to benefit the weevil in other ways, such as increasing the nutritional properties of their diet.

scholarly journals Emergence of nosocomial associated opportunistic pathogens in the gut microbiome after antibiotic treatment

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Isaac Raplee ◽  
Lacey Walker ◽  
Lei Xu ◽  
Anil Surathu ◽  
Ashok Chockalingam ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Antibiotic Therapy ◽  
Antibiotic Treatment ◽  
Nosocomial Infections ◽  
Pathogenic Bacteria ◽  
Antibiotic Administration ◽  
Oral Antibiotic ◽  
Opportunistic Pathogens ◽  
Taxonomic Assignment ◽  
Antibiotic Resistant

Abstract Introduction According to the Centers for Disease Control’s 2015 Hospital Acquired Infection Hospital Prevalence Survey, 1 in 31 hospital patients was infected with at least one nosocomial pathogen while being treated for unrelated issues. Many studies associate antibiotic administration with nosocomial infection occurrence. However, to our knowledge, there is little to no direct evidence of antibiotic administration selecting for nosocomial opportunistic pathogens. Aim This study aims to confirm gut microbiota shifts in an animal model of antibiotic treatment to determine whether antibiotic use favors pathogenic bacteria. Methodology We utilized next-generation sequencing and in-house metagenomic assembly and taxonomic assignment pipelines on the fecal microbiota of a urinary tract infection mouse model with and without antibiotic treatment. Results Antibiotic therapy decreased the number of detectable species of bacteria by at least 20-fold. Furthermore, the gut microbiota of antibiotic treated mice had a significant increase of opportunistic pathogens that have been implicated in nosocomial infections, like Acinetobacter calcoaceticus/baumannii complex, Chlamydia abortus, Bacteroides fragilis, and Bacteroides thetaiotaomicron. Moreover, antibiotic treatment selected for antibiotic resistant gene enriched subpopulations for many of these opportunistic pathogens. Conclusions Oral antibiotic therapy may select for common opportunistic pathogens responsible for nosocomial infections. In this study opportunistic pathogens present after antibiotic therapy harbored more antibiotic resistant genes than populations of opportunistic pathogens before treatment. Our results demonstrate the effects of antibiotic therapy on induced dysbiosis and expansion of opportunistic pathogen populations and antibiotic resistant subpopulations of those pathogens. Follow-up studies with larger samples sizes and potentially controlled clinical investigations should be performed to confirm our findings.

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