scholarly journals Simultaneous Detection of Bovine Rotavirus, Bovine Parvovirus, and Bovine Viral Diarrhea Virus Using a Gold Nanoparticle-Assisted PCR Assay With a Dual-Priming Oligonucleotide System

2019 ◽  
Vol 10 ◽  
Author(s):  
Mengmeng Wang ◽  
Yue Yan ◽  
Ruichong Wang ◽  
Li Wang ◽  
Han Zhou ◽  
...  
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Simultaneous Detection ◽  
Bovine Viral Diarrhea ◽  
Pcr Assay ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Bovine Rotavirus ◽  
Dual Priming Oligonucleotide ◽  
Bovine Parvovirus ◽  
Viral Diarrhea Virus

scholarly journals Multiplex real-time RT-PCR assay for bovine viral diarrhea virus type 1, type 2 and HoBi-like pestivirus

2016 ◽  
Vol 229 ◽  
pp. 1-7 ◽  
Author(s):  
Viviana Mari ◽  
Michele Losurdo ◽  
Maria Stella Lucente ◽  
Eleonora Lorusso ◽  
Gabriella Elia ◽  
...  
Keyword(s):  
Real Time ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus

scholarly journals Double-immunolabeling systems for phenotyping of immune cells harboring bovine viral diarrhea virus.

1987 ◽  
Vol 35 (6) ◽  
pp. 627-633 ◽  
Author(s):  
H Bielefeldt Ohmann
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Simultaneous Detection ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Tissue Sections ◽  
Specificity And Sensitivity ◽  
Infected Cells ◽  
Viral Diarrhea Virus ◽  
Double Immunolabeling

Optimal staining conditions were defined for simultaneous detection of bovine viral diarrhea virus (BVDV) and mononuclear leukocyte surface antigens in tissue sections and cytospins. Because of the extreme lability of the virus antigens and the variable stability of the epitopes on the cell differentiation antigens, cryopreservation had to be used. This method gives slightly sub-optimal preservation of morphology. However, the specificity and sensitivity of the immunolabeling ensured reliable identification of the double-labeled cells, i.e., the phenotypic identification of virus-infected cells within the immune system.

Bovine viral diarrhea virus subgenotype 1a in a mummified fetus from a Brazilian dairy cattle herd

2021 ◽  
pp. 104063872110220
Author(s):  
Juliana T. T. Fritzen ◽  
Arthur B. Morettin ◽  
Elis Lorenzetti ◽  
Alice F. Alfieri ◽  
Amauri A. Alfieri
Keyword(s):  
Dairy Cattle ◽  
Bovine Viral Diarrhea Virus ◽  
Cattle Herd ◽  
Bovine Viral Diarrhea ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Dairy Cattle Herd ◽  
Viral Diarrhea Virus

We describe the molecular analysis of a wild-type field strain of bovine viral diarrhea virus (BVDV) identified in a mummified fetus from a small Brazilian dairy cattle herd. Nucleic acids extracted from samples of the lung, liver, heart, spleen, and kidney were tested by PCR assays for bovine alphaherpesvirus 1, Neospora caninum, Leptospira spp., Histophilus somni, and Brucella abortus, a nested PCR assay for Mycoplasma bovigenitalium and Ureaplasma diversum, and a RT-PCR assay for BVDV. Amplicons were only obtained in the RT-PCR assay for the partial amplification of the BVDV 5′UTR (288 bp) in kidney and spleen samples and the Npro (438 bp) gene in the kidney sample. Nucleotide sequencing of the amplified products and phylogenetic analyses based on the 2 BVDV genomic regions enabled the BVDV strain to be classified as subgenotype 1a.

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