Effect of infection with bovine viral diarrhea virus alone, bovine rotavirus alone, or concurrent infection with both on enteric disease in gnotobiotic neonatal calves

2002 ◽  
Vol 63 (8) ◽  
pp. 1179-1186 ◽  
Author(s):  
Clayton L. Kelling ◽  
David J. Steffen ◽  
Vickie L. Cooper ◽  
Deborrah S. Higuchi ◽  
Kent M. Eskridge
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Concurrent Infection ◽  
Enteric Disease ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Bovine Rotavirus ◽  
Neonatal Calves ◽  
Viral Diarrhea Virus

scholarly journals Screening of Neonatal Calves for Persistent Infection with Bovine Viral Diarrhea Virus by Immunohistochemistry on Skin Biopsy Samples

2002 ◽  
Vol 9 (4) ◽  
pp. 898-900 ◽  
Author(s):  
Daniel L. Grooms ◽  
Eric D. Keilen
Keyword(s):  
Skin Biopsy ◽  
Bovine Viral Diarrhea Virus ◽  
Immunohistochemical Staining ◽  
Bovine Viral Diarrhea ◽  
Persistently Infected ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Neonatal Calves ◽  
Viral Diarrhea Virus

ABSTRACT Detection and elimination of cattle that are persistently infected with bovine viral diarrhea virus (BVDV) is important for controlling the transmission of this virus. Colostrum-derived antibodies make the detection of persistently BVDV-infected neonatal calves cumbersome and expensive. The objective of this study was to evaluate the use of immunohistochemical staining of skin biopsy samples from neonatal calves as a method for the early detection of persistent BVDV infection. Three hundred thirty-two 1- to 4-week-old dairy calves were screened for BVDV as part of a routine control program. Formalin-fixed skin biopsy samples were stained for BVDV antigen by immunohistochemistry (IHC), and the results were compared to those of virus isolation (VI) from white blood cell preparations. Six calves were positive by both IHC and VI and remained positive for BVDV upon subsequent follow-up testing; thus, they were classified as persistently infected with BVDV. One calf was positive by VI but negative by IHC. On subsequent testing, the calf was negative by VI, suggesting that the initial VI result was due to an acute BVDV infection. One calf was positive by IHC but negative by VI. This calf remained negative by VI on follow-up testing. Immunohistochemical staining of skin biopsy samples is a reliable method for screening neonatal calves for persistent BVDV infection and would be a useful management tool as an aid for controlling and preventing BVDV infection.

scholarly journals Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period

2020 ◽  
Vol 51 (4) ◽  
pp. 2077-2086 ◽  
Author(s):  
Maximiliano J. Spetter ◽  
Enrique L. Louge Uriarte ◽  
Joaquín I. Armendano ◽  
Eleonora L. Morrell ◽  
Germán J. Cantón ◽  
...  
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Detection Methods ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Neonatal Calves ◽  
Viral Diarrhea Virus

Diagnosis of Naturally Occurring Bovine Viral Diarrhea Virus Infections in Ruminants Using Monoclonal Antibody-based Immunohistochemistry

1995 ◽  
Vol 32 (6) ◽  
pp. 609-618 ◽  
Author(s):  
T. V. Baszler ◽  
J. F. Evermann ◽  
P. S. Kaylor ◽  
T. C. Byington ◽  
P. M. Dilbeck
Keyword(s):  
Monoclonal Antibody ◽  
Respiratory Disease ◽  
Bovine Viral Diarrhea Virus ◽  
Virus Isolation ◽  
Clinical Disease ◽  
Bovine Viral Diarrhea ◽  
Enteric Disease ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus

Formalin-fixed paraffin-embedded tissues from 50 spontaneous cases (39 bovine, nine ovine, two caprine) of bovine viral diarrhea virus (BVDV) infection diagnosed by virus isolation were retrospectively examined for BVDV antigen by immunohistochemistry using anti-BVDV gp-43 monoclonal antibody (Mab 15C5). The cases were separated into enteric disease syndrome, respiratory disease syndrome, and abortion/weak calf syndrome based upon clinical disease. The purposes of the study were to 1) compare routine virus isolation with immunohistochemistry in determining BVDV infection and 2) define tissue and cellular distribution of BVDV in various clinical manifestations of infection. In bovids, there was 100% concordance of virus isolation and immunohistochemistry using Mab 15C5 in cases of enteric disease (mucosal disease, acute and chronic diarrhea, neonatal diarrhea), respiratory disease, and abortion. When laboratory tests were restricted to gastrointestinal tissue and/or feces, virus isolation detected BVDV in only 65% of cattle, whereas immunohistochemistry detected BVDV antigen in 100% of cattle. Immunohistochemical detection of pestivirus was poor in cases of ovine abortion, ovine hairy shaker syndrome, and caprine abortion. The tissue distribution of BVDV antigen was widespread in individual cattle with all clinical forms of BVDV infection. Viral antigen accumulation was spatially correlated with tissue lesions (in the absence of other pathogens) only in the gastrointestinal tract, lymphoid tissue, lung, placenta, and eye. This study demonstrates the utility of immunohistochemistry using Mab 15C5 to diagnose BVDV infections in cattle with a broad spectrum of clinical disease.

scholarly journals Experimental Model of Type II Bovine Viral Diarrhea Virus-Induced Thrombocytopenia in Neonatal Calves

1999 ◽  
Vol 11 (6) ◽  
pp. 505-514 ◽  
Author(s):  
Paul H. Walz ◽  
Thomas G. Bell ◽  
Barbara A. Steficek ◽  
Lana Kaiser ◽  
Roger K. Maes ◽  
...  
Keyword(s):  
Experimental Model ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Type Ii ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Neonatal Calves ◽  
Viral Diarrhea Virus

Prevention and elimination of bovine viral diarrhea virus infections in fetal fibroblast cells

2004 ◽  
Vol 64 (2) ◽  
pp. 113-118 ◽  
Author(s):  
M GIVENS ◽  
D STRINGFELLOW ◽  
C DYKSTRA ◽  
K RIDDELL ◽  
P GALIK ◽  
...  
Keyword(s):  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Fibroblast Cells ◽  
Virus Infections ◽  
Fetal Fibroblast ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus
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