scholarly journals Characterization of the Cultivable Microbiota in Fresh and Stored Mature Human Breast Milk

2019 ◽  
Vol 10 ◽  
Author(s):  
Clarissa Schwab ◽  
Evelyn Voney ◽  
Alejandro Ramirez Garcia ◽  
Michaela Vischer ◽  
Christophe Lacroix
Keyword(s):  
Breast Milk ◽  
Human Breast Milk ◽  
Human Breast

scholarly journals Characterization of Biosurfactant Produced by Probiotic Bacteria Isolated from Human Breast Milk

2018 ◽  
Vol 3 (1) ◽  
pp. 18-24
Author(s):  
Laya Tabrizi Rayeni ◽  
Shahla Soltani Nezhad
Keyword(s):  
Breast Milk ◽  
Gene Sequencing ◽  
Human Breast Milk ◽  
Sequencing Analysis ◽  
Human Breast ◽  
16S Rdna Gene ◽  
Pediococcus Pentosaceus ◽  
Milk Samples ◽  
16S Rdna Gene Sequencing

Background: Breast milk is an important nutrient source for rapidly growing neonates since breastfeeding protects the newborn against some disease. This effect may be due to the useful and natural microflora of breast milk. Biosurfactants are unique amphipathic compounds produced by some microorganisms. The present study demonstrates the isolation and characterization of biosurfactant producing bacteria from human breast milk samples. Methods: The human breast milk samples were collected aseptically and then cultured in MRS agar media. The biosurfactant producing ability of the isolated strains was investigated by hemolytic assay, oil spreading method, drop collapse test and emulsification index assay. The screened isolates were identified by 16S rDNA gene sequencing analysis. In vitro antibacterial activities of biosurfactants against some Gram-positive and Gram negative bacteria were investigated by the agar disc diffusion method. This biosurfactant was characterized with Fourier transform infrared spectroscopy (FTIR). Results: In this study, 337 different colonies were isolated from 42 breast milk samples. The best isolates were identified as Pediococcus pentosaceus HM-1, Pediococcus pentosaceus HM-2 and Pediococcus pentosaceus HM-3 based on microscopic and 16S rDNA gene sequencing analysis. The biosurfactant extracted from screened strains exhibited a broad spectrum of antagonistic activity against some pathogenic bacteria. The results showed similarity to lipopeptide biosurfactants like surfactin. Conclusion: Bacterial strains isolated in this study could be valuable sources for novel biosurfactants. The Human breast milk could be a safe source for isolation of biosurfactant producing probiotic bacteria and for improve intestinal microflora of infants.

Characterization of Human Breast Milk Leukocytes and Their Potential Role in Cytomegalovirus Transmission to Newborns

Neonatology ◽  
2015 ◽  
Vol 107 (3) ◽  
pp. 213-219 ◽  
Author(s):  
Jens Maschmann ◽  
Rangmar Goelz ◽  
Simone Witzel ◽  
Ute Strittmatter ◽  
Max Steinmassl ◽  
...  
Keyword(s):  
Breast Milk ◽  
Potential Role ◽  
Human Breast Milk ◽  
Human Breast

Characterization of an exopolysaccharide produced by Lactobacillus plantarum HM47 isolated from human breast milk

2018 ◽  
Vol 73 ◽  
pp. 15-22 ◽  
Author(s):  
Reshma B Nambiar ◽  
Periyar Selvam Sellamuthu ◽  
Anand Babu Perumal ◽  
Emmanuel Rotimi Sadiku ◽  
Goitse Phiri ◽  
...  
Keyword(s):  
Breast Milk ◽  
Lactobacillus Plantarum ◽  
Human Breast Milk ◽  
Human Breast
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