Characterization of Human Breast Milk Leukocytes and Their Potential Role in Cytomegalovirus Transmission to Newborns

Neonatology ◽  
2015 ◽  
Vol 107 (3) ◽  
pp. 213-219 ◽  
Author(s):  
Jens Maschmann ◽  
Rangmar Goelz ◽  
Simone Witzel ◽  
Ute Strittmatter ◽  
Max Steinmassl ◽  
...  
Keyword(s):  
Breast Milk ◽  
Potential Role ◽  
Human Breast Milk ◽  
Human Breast

scholarly journals Mycobiome Profiles in Breast Milk from Healthy Women Depend on Mode of Delivery, Geographic Location, and Interaction with Bacteria

2019 ◽  
Vol 85 (9) ◽  
Author(s):  
Alba Boix-Amorós ◽  
Fernando Puente-Sánchez ◽  
Elloise du Toit ◽  
Kaisa M. Linderborg ◽  
Yumei Zhang ◽  
...  
Keyword(s):  
Breast Milk ◽  
Potential Role ◽  
Mode Of Delivery ◽  
Geographical Location ◽  
Geographic Location ◽  
Fungal Species ◽  
Human Breast Milk ◽  
Human Breast ◽  
Its1 Region ◽  
Milk Samples

ABSTRACTRecent studies report the presence of fungal species in breast milk of healthy mothers, suggesting a potential role in infant mycobiome development. In the present work, we aimed to determine whether the healthy human breast milk mycobiota is influenced by geographical location and mode of delivery, as well as to investigate its interaction with bacterial profiles in the same samples. A total of 80 mature breast milk samples from 4 different countries were analyzed by Illumina sequencing of the internal transcribed spacer 1 (ITS1) region, joining the 18S and 5.8S regions of the fungal rRNA region. Basidiomycota and Ascomycota were found to be the dominant phyla, withMalasseziaandDavidiellabeing the most prevalent genera across countries. A core formed byMalassezia, Davidiella, Sistotrema, andPenicilliumwas shared in the milk samples from the different origins, although specific shifts in mycobiome composition were associated with geographic location and delivery mode. The presence of fungi in the breast milk samples was further confirmed by culture and isolate characterization, and fungal loads were estimated by quantitative PCR (qPCR) targeting the fungal ITS1 region. Cooccurrence network analysis of bacteria and fungi showed complex interactions that were influenced by geographical location, mode of delivery, maternal age, and pregestational body mass index. The presence of a breast milk mycobiome was confirmed in all samples analyzed, regardless of the geographic origin.IMPORTANCEDuring recent years, human breast milk has been documented as a potential source of bacteria for the newborn. Recently, we have reported the presence of fungi in breast milk from healthy mothers. It is well known that environmental and perinatal factors can affect milk bacteria; however, the impact on milk fungi is still unknown. The current report describes fungal communities (mycobiota) in breast milk samples across different geographic locations and the influence of the mode of delivery. We also provide novel insights on bacterium-fungus interactions, taking into account environmental and perinatal factors. We identified a core of four genera shared across locations, consisting ofMalassezia, Davidiella, Sistotrema, andPenicillium, which have been reported to be present in the infant gut. Our data confirm the presence of fungi in breast milk across continents and support the potential role of breast milk in the initial seeding of fungal species in the infant gut.

scholarly journals Characterization of the Cultivable Microbiota in Fresh and Stored Mature Human Breast Milk

2019 ◽  
Vol 10 ◽  
Author(s):  
Clarissa Schwab ◽  
Evelyn Voney ◽  
Alejandro Ramirez Garcia ◽  
Michaela Vischer ◽  
Christophe Lacroix
Keyword(s):  
Breast Milk ◽  
Human Breast Milk ◽  
Human Breast

scholarly journals Characterization of Biosurfactant Produced by Probiotic Bacteria Isolated from Human Breast Milk

2018 ◽  
Vol 3 (1) ◽  
pp. 18-24
Author(s):  
Laya Tabrizi Rayeni ◽  
Shahla Soltani Nezhad
Keyword(s):  
Breast Milk ◽  
Gene Sequencing ◽  
Human Breast Milk ◽  
Sequencing Analysis ◽  
Human Breast ◽  
16S Rdna Gene ◽  
Pediococcus Pentosaceus ◽  
Milk Samples ◽  
16S Rdna Gene Sequencing

Background: Breast milk is an important nutrient source for rapidly growing neonates since breastfeeding protects the newborn against some disease. This effect may be due to the useful and natural microflora of breast milk. Biosurfactants are unique amphipathic compounds produced by some microorganisms. The present study demonstrates the isolation and characterization of biosurfactant producing bacteria from human breast milk samples. Methods: The human breast milk samples were collected aseptically and then cultured in MRS agar media. The biosurfactant producing ability of the isolated strains was investigated by hemolytic assay, oil spreading method, drop collapse test and emulsification index assay. The screened isolates were identified by 16S rDNA gene sequencing analysis. In vitro antibacterial activities of biosurfactants against some Gram-positive and Gram negative bacteria were investigated by the agar disc diffusion method. This biosurfactant was characterized with Fourier transform infrared spectroscopy (FTIR). Results: In this study, 337 different colonies were isolated from 42 breast milk samples. The best isolates were identified as Pediococcus pentosaceus HM-1, Pediococcus pentosaceus HM-2 and Pediococcus pentosaceus HM-3 based on microscopic and 16S rDNA gene sequencing analysis. The biosurfactant extracted from screened strains exhibited a broad spectrum of antagonistic activity against some pathogenic bacteria. The results showed similarity to lipopeptide biosurfactants like surfactin. Conclusion: Bacterial strains isolated in this study could be valuable sources for novel biosurfactants. The Human breast milk could be a safe source for isolation of biosurfactant producing probiotic bacteria and for improve intestinal microflora of infants.

Soluble CD14 concentration in human breast milk and its potential role in child atopic dermatitis: Results of the Ulm Birth Cohort Studies

2018 ◽  
Vol 49 (2) ◽  
pp. 199-206 ◽  
Author(s):  
Chad A. Logan ◽  
Johannes M. Weiss ◽  
Wolfgang Koenig ◽  
Bernd Stahl ◽  
Prudence R. Carr ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Breast Milk ◽  
Cohort Studies ◽  
Birth Cohort ◽  
Potential Role ◽  
Human Breast Milk ◽  
Human Breast ◽  
Soluble Cd14

Characterization of an exopolysaccharide produced by Lactobacillus plantarum HM47 isolated from human breast milk

2018 ◽  
Vol 73 ◽  
pp. 15-22 ◽  
Author(s):  
Reshma B Nambiar ◽  
Periyar Selvam Sellamuthu ◽  
Anand Babu Perumal ◽  
Emmanuel Rotimi Sadiku ◽  
Goitse Phiri ◽  
...  
Keyword(s):  
Breast Milk ◽  
Lactobacillus Plantarum ◽  
Human Breast Milk ◽  
Human Breast
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