scholarly journals Corrigendum: The Response of Cupriavidus metallidurans CH34 to Cadmium Involves Inhibition of the Initiation of Biofilm Formation, Decrease in Intracellular c-di-GMP Levels, and a Novel Metal Regulated Phosphodiesterase

2019 ◽  
Vol 10 ◽  
Author(s):  
Pablo Alviz-Gazitua ◽  
Sebastián Fuentes-Alburquenque ◽  
Luis A. Rojas ◽  
Raymond J. Turner ◽  
Nicolas Guiliani ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Cupriavidus Metallidurans Ch34 ◽  
Cupriavidus Metallidurans

scholarly journals The Response of Cupriavidus Metallidurans CH34 to Cadmium Involves a Decrease in Intracellular Levels of C-Di-GMP Probably Mediated by a Novel Metal Regulated Phosphodiesterase That Inhibits the Biofilm Lifestyle

2018 ◽  
Author(s):  
Pablo Alviz ◽  
Sebastian Fuentes ◽  
Luis Rojas ◽  
Raymond Turner ◽  
Michael Seeger ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Biofilm Formation ◽  
Bioinformatic Analysis ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Cadmium Resistance ◽  
Toxic Heavy Metal ◽  
Transcription Analysis ◽  
Cupriavidus Metallidurans Ch34 ◽  
Cupriavidus Metallidurans

Cadmium is a highly toxic heavy metal for biological systems. Cupriavidus metallidurans CH34 is a model strain for heavy metal resistance and bioremediation. The aim of this study was to determine the role of the c-di-GMP pathway in the C. metallidurans CH34 response to cadmium in both planktonic and biofilm cells. Increasing cadmium concentrations correlates with an inhibition of biofilm formation and EPS production in C. metallidurans cells. Planktonic and biofilm cells showed similar tolerance to cadmium. During exposure to cadmium an acute decrease of c-di-GMP levels in planktonic and biofilm cells was observed. Transcription analysis by RT-qPCR showed that cadmium induced in planktonic cells and strongly induced in biofilm cells the expression of the urf2 gene and the mercuric reductase encoding merA gene, which belong to the Tn501/Tn21 mer operon. After exposure to cadmium the cadA gene involved in cadmium resistance was equally upregulated in both lifestyles. Bioinformatic analysis and null mutant complementation assays indicated that the protein encoded by the urf2 gene is a functional phosphodiesterase involved in the c-di-GMP metabolism. We propose to rename the urf2 gene as mrp gene for metal regulated phosphodiesterase. An increase of the second messenger c-di-GMP content by the heterologous expression of the constitutively active diguanylate cyclase PleD* correlated with an increase in biofilm formation and cadmium susceptibility. These results indicate that the response to cadmium in C. metallidurans CH34 involves a decrease in c-di-GMP content that inhibits the biofilm lifestyle.

CopK from Cupriavidus metallidurans CH34 Binds Cu(I) in a Tetrathioether Site: Characterization by X-ray Absorption and NMR Spectroscopy

2010 ◽  
Vol 132 (11) ◽  
pp. 3770-3777 ◽  
Author(s):  
Géraldine Sarret ◽  
Adrien Favier ◽  
Jacques Covès ◽  
Jean-Louis Hazemann ◽  
Max Mergeay ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Site Characterization ◽  
Cupriavidus Metallidurans Ch34 ◽  
X Ray ◽  
Cupriavidus Metallidurans ◽  
X Ray Absorption

Precipitation of Silver-Thiosulfate Complex and Immobilization of Silver by Cupriavidus metallidurans CH34

BioMetals ◽  
2005 ◽  
Vol 18 (6) ◽  
pp. 643-650 ◽  
Author(s):  
Marie-Laure Ledrich ◽  
Sébastien Stemmler ◽  
Philippe Laval-Gilly ◽  
Laurent Foucaud ◽  
Jaïro Falla
Keyword(s):  
Cupriavidus Metallidurans Ch34 ◽  
Silver Thiosulfate ◽  
Cupriavidus Metallidurans

scholarly journals Proteomic responses to gold(iii)-toxicity in the bacterium Cupriavidus metallidurans CH34

Metallomics ◽  
2016 ◽  
Vol 8 (11) ◽  
pp. 1204-1216 ◽  
Author(s):  
Carla M. Zammit ◽  
Florian Weiland ◽  
Joël Brugger ◽  
Benjamin Wade ◽  
Lyron Juan Winderbaum ◽  
...  
Keyword(s):  
Cupriavidus Metallidurans Ch34 ◽  
Cupriavidus Metallidurans ◽  
Proteomic Responses
Sign in / Sign up

Export Citation Format

Share Document