Gold-Specific Biosensor for Monitoring Wastewater Using Genetically Engineered Cupriavidus metallidurans CH34

2021 ◽  
Author(s):  
Ssu-Tzu Tsai ◽  
Wen-Jui Cheng ◽  
Qian-Xian Zhang ◽  
Yi-Chun Yeh
Keyword(s):  
Genetically Engineered ◽  
Cupriavidus Metallidurans Ch34 ◽  
Cupriavidus Metallidurans

CopK from Cupriavidus metallidurans CH34 Binds Cu(I) in a Tetrathioether Site: Characterization by X-ray Absorption and NMR Spectroscopy

2010 ◽  
Vol 132 (11) ◽  
pp. 3770-3777 ◽  
Author(s):  
Géraldine Sarret ◽  
Adrien Favier ◽  
Jacques Covès ◽  
Jean-Louis Hazemann ◽  
Max Mergeay ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Site Characterization ◽  
Cupriavidus Metallidurans Ch34 ◽  
X Ray ◽  
Cupriavidus Metallidurans ◽  
X Ray Absorption

Precipitation of Silver-Thiosulfate Complex and Immobilization of Silver by Cupriavidus metallidurans CH34

BioMetals ◽  
2005 ◽  
Vol 18 (6) ◽  
pp. 643-650 ◽  
Author(s):  
Marie-Laure Ledrich ◽  
Sébastien Stemmler ◽  
Philippe Laval-Gilly ◽  
Laurent Foucaud ◽  
Jaïro Falla
Keyword(s):  
Cupriavidus Metallidurans Ch34 ◽  
Silver Thiosulfate ◽  
Cupriavidus Metallidurans

scholarly journals Proteomic responses to gold(iii)-toxicity in the bacterium Cupriavidus metallidurans CH34

Metallomics ◽  
2016 ◽  
Vol 8 (11) ◽  
pp. 1204-1216 ◽  
Author(s):  
Carla M. Zammit ◽  
Florian Weiland ◽  
Joël Brugger ◽  
Benjamin Wade ◽  
Lyron Juan Winderbaum ◽  
...  
Keyword(s):  
Cupriavidus Metallidurans Ch34 ◽  
Cupriavidus Metallidurans ◽  
Proteomic Responses

scholarly journals Seleno-l-Methionine Is the Predominant Organic Form of Selenium in Cupriavidus metallidurans CH34 Exposed to Selenite or Selenate

2006 ◽  
Vol 72 (9) ◽  
pp. 6414-6416 ◽  
Author(s):  
Laure Avoscan ◽  
Richard Collins ◽  
Marie Carriere ◽  
Barbara Gouget ◽  
Jacques Cov�s
Keyword(s):  
Inductively Coupled Plasma ◽  
High Performance ◽  
Organic Form ◽  
Edge Structure ◽  
Cupriavidus Metallidurans Ch34 ◽  
X Ray ◽  
Inductively Coupled ◽  
Cupriavidus Metallidurans ◽  
Plasma Mass Spectrometry ◽  
X Ray Absorption

ABSTRACT The accumulated organic form of selenium previously detected by X-ray absorption near-edge structure (XANES) analyses in Cupriavidus metallidurans CH34 exposed to selenite or selenate was identified as seleno-l-methionine by coupling high-performance liquid chromatography to inductively coupled plasma-mass spectrometry.

scholarly journals Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters

2020 ◽  
Author(s):  
Esmail Nourmohammadi ◽  
Saman Hosseinkhani ◽  
Reza Nedaeinia ◽  
Hoda Khoshdel-Sarkarizi ◽  
Mozhdeh Nedaeinia ◽  
...  
Keyword(s):  
Genetically Engineered ◽  
Luciferase Reporter ◽  
Chemical Agent ◽  
Luciferase Reporter Gene ◽  
Bacterial Biosensor ◽  
Cupriavidus Metallidurans ◽  
Zinc Cadmium ◽  
Genetically Engineered Bacteria ◽  
Environmental And Biological Samples ◽  
Engineered Bacteria

Abstract Background: A bacterial biosensor refers to genetically engineered bacteria that produce an assessable signal in the presence of a physical or chemical agent in the environment.Methods: We have designed and evaluated a bacterial biosensor expressing a luciferase-reporter gene controlled by pbr and cadA promoters in Cupriavidus metallidurans (previously termed Ralstonia metallidurans ) containing the CH34 and pI258 plasmids of Staphylococcus aureus , respectively, and that can be used for the detection of heavy metals. In the present study, we have produced and evaluated biosensor plasmids designated pGL3-luc/pbr -biosensor and pGL3-luc/cad -biosensor, that were based on the expression of luc+ under the control of the cad promoter and the cadC gene of S. aureus plasmid pI258 and pbr promoter and pbrR gene from plasmid pMOL30 of Cupriavidus metallidurans.Results: We found that the pGL3-luc/pbr -biosensor may be used to measure lead concentrations between 1-100 μM in the presence of other metals, including: zinc, cadmium, tin and nickel. The latter metals did not result in any significant signal gene expression of the reporter. The pGL3-luc/cad -biosensor could detect lead concentrations between 10 nM to 10 μM.Conclusions: This biosensor was found to be a specific for measuring lead ions in both environmental and biological samples.

scholarly journals Construction of a sensitive and specific lead biosensor using a genetically engineered bacterial system with a luciferase gene reporter controlled by pbr and cadA promoters

2020 ◽  
Author(s):  
Esmail Nourmohammadi ◽  
Saman Hosseinkhani ◽  
Reza Nedaeinia ◽  
Hoda Khoshdel-Sarkarizi ◽  
Mozhdeh Nedaeinia ◽  
...  
Keyword(s):  
Genetically Engineered ◽  
Luciferase Reporter ◽  
Chemical Agent ◽  
Luciferase Reporter Gene ◽  
Bacterial Biosensor ◽  
Cupriavidus Metallidurans ◽  
Zinc Cadmium ◽  
Genetically Engineered Bacteria ◽  
Environmental And Biological Samples ◽  
Engineered Bacteria

Abstract Background: A bacterial biosensor refers to genetically engineered bacteria that produce an assessable signal in the presence of a physical or chemical agent in the environment.Methods: We have designed and evaluated a bacterial biosensor expressing a luciferase-reporter gene controlled by pbr and cadA promoters in Cupriavidus metallidurans (previously termed Ralstonia metallidurans ) containing the CH34 and pI258 plasmids of Staphylococcus aureus , respectively, and that can be used for the detection of heavy metals. In the present study, we have produced and evaluated biosensor plasmids designated pGL3-luc/pbr -biosensor and pGL3-luc/cad -biosensor, that were based on the expression of luc+ under the control of the cad promoter and the cadC gene of S. aureus plasmid pI258 and pbr promoter and pbrR gene from plasmid pMOL30 of Cupriavidus metallidurans.Results: We found that the pGL3-luc/pbr -biosensor may be used to measure lead concentrations between 1-100 μM in the presence of other metals, including: zinc, cadmium, tin and nickel. The latter metals did not result in any significant signal gene expression of the reporter . The pGL3-luc/cad -biosensor could detect lead concentrations between 10 nM to 10 μM.Conclusions: This biosensor was found to be a specific for measuring lead ions in both environmental and biological samples.

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