scholarly journals Surface Exclusion Revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20

2019 ◽  
Vol 10 ◽  
Author(s):  
César Gago-Córdoba ◽  
Jorge Val-Calvo ◽  
Andrés Miguel-Arribas ◽  
Ester Serrano ◽  
Praveen K. Singh ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Differential Expression ◽  
Surface Exclusion

Differential expression of ASS1 in serous and non-serous ovarian carcinomas

2014 ◽  
pp. n/a-n/a ◽  
Author(s):  
Dong-Joo Cheon ◽  
Ann E. Walts ◽  
Jessica A. Beach ◽  
Jenny Lester ◽  
John S. Bomalaski ◽  
...  
Keyword(s):  
Differential Expression ◽  
Ovarian Carcinomas

Use of Antibody to aid Visualization of Protein at the Termini of Bacteriophage Ø29 DNA

1980 ◽  
Vol 38 ◽  
pp. 540-541
Author(s):  
Dwight Anderson ◽  
Charlene Peterson ◽  
Gursaran Notani ◽  
Bernard Reilly
Keyword(s):  
Electron Microscopy ◽  
Bacillus Subtilis ◽  
Dna Synthesis ◽  
Restriction Endonuclease ◽  
Protein Complex ◽  
Protein Product ◽  
Viral Dna ◽  
Small Proteins ◽  
Antibody Labeling ◽  
Subtilis Bacteriophage

The protein product of cistron 3 of Bacillus subtilis bacteriophage Ø29 is essential for viral DNA synthesis and is covalently bound to the 5’-termini of the Ø29 DNA. When the DNA-protein complex is cleaved with a restriction endonuclease, the protein is bound to the two terminal fragments. The 28,000 dalton protein can be visualized by electron microscopy as a small dot and often is seen only when two ends are in apposition as in multimers or in glutaraldehyde-fixed aggregates. We sought to improve the visibility of these small proteins by use of antibody labeling.

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