scholarly journals Microbial Diversity in Bulk and Rhizosphere Soil of Ranunculus glacialis Along a High-Alpine Altitudinal Gradient

2019 ◽  
Vol 10 ◽  
Author(s):  
Nadine Praeg ◽  
Harald Pauli ◽  
Paul Illmer
Keyword(s):  
Microbial Diversity ◽  
Altitudinal Gradient ◽  
Rhizosphere Soil ◽  
Ranunculus Glacialis

scholarly journals A Degeneration Gradient of Poplar Trees Contributes to the Taxonomic, Functional, and Resistome Diversity of Bacterial Communities in Rhizosphere Soils

2021 ◽  
Vol 22 (7) ◽  
pp. 3438
Author(s):  
Juan Liu ◽  
Xiangwei He ◽  
Jingya Sun ◽  
Yuchao Ma
Keyword(s):  
Soil Fertility ◽  
Microbial Diversity ◽  
Resistance Genes ◽  
Bacterial Communities ◽  
Rhizosphere Soil ◽  
Antibiotics Resistance ◽  
Plant Growth Promoting Bacteria ◽  
Soil Microbial Diversity ◽  
Antibiotic Target ◽  
And Function

Bacterial communities associated with roots influence the health and nutrition of the host plant. However, the microbiome discrepancy are not well understood under different healthy conditions. Here, we tested the hypothesis that rhizosphere soil microbial diversity and function varies along a degeneration gradient of poplar, with a focus on plant growth promoting bacteria (PGPB) and antibiotic resistance genes. Comprehensive metagenomic analysis including taxonomic investigation, functional detection, and ARG (antibiotics resistance genes) annotation revealed that available potassium (AK) was correlated with microbial diversity and function. We proposed several microbes, Bradyrhizobium, Sphingomonas, Mesorhizobium, Nocardioides, Variovorax, Gemmatimonadetes, Rhizobacter, Pedosphaera, Candidatus Solibacter, Acidobacterium, and Phenylobacterium, as candidates to reflect the soil fertility and the plant health. The highest abundance of multidrug resistance genes and the four mainly microbial resistance mechanisms (antibiotic efflux, antibiotic target protection, antibiotic target alteration, and antibiotic target replacement) in healthy poplar rhizosphere, corroborated the relationship between soil fertility and microbial activity. This result suggested that healthy rhizosphere soil harbored microbes with a higher capacity and had more complex microbial interaction network to promote plant growing and reduce intracellular levels of antibiotics. Our findings suggested a correlation between the plant degeneration gradient and bacterial communities, and provided insight into the role of high-turnover microbial communities as well as potential PGPB as real-time indicators of forestry soil quality, and demonstrated the inner interaction contributed by the bacterial communities.

scholarly journals Microbial diversity in rhizosphere soil of soybean grass under different cultivation methods in an alpine region

2020 ◽  
Author(s):  
Ying Zhang ◽  
Wenhui Liu ◽  
Xilai Li ◽  
Zhiying Zhang ◽  
Beibei Su ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microbial Diversity ◽  
Avena Sativa ◽  
Rhizosphere Soil ◽  
Soil Microbial Communities ◽  
Culture Method ◽  
Alpine Region ◽  
Vicia Sativa ◽  
Seeding Method ◽  
Cultivation Methods

Abstract Background A large number of studies have shown that soybean grass with mixed seeding cultivation can significantly improve the yield and quality of forage grass compared with clean culture cultivation.This study explores the differences in the characteristics of the composition and diversity of the microbial community in the rhizosphere of soybean grasses between clean culture and mixed seeding methods in an alpine region. We used high-throughput sequencing technology to determine the microbial diversity and analytical methods to determine the physicochemical characteristics of plant rhizosphere soil of Avena sativa L. and Vicia sativa L. Results There were no significant differences in pH, total nitrogen, total phosphorus, and total potassium in the rhizosphere soil samples of soybean grasses under the clean culture and mixed seeding methods, while there were significant differences in the available nitrogen, available phosphorus, available potassium, and organic matter content (P < 0.05). The bacterial diversity of the rhizosphere soil of Avena sativa L. was the highest under the clean culture method, and the fungal diversity of the rhizosphere soil of Vicia sativa L. was the highest under the clean culture method. Furthermore, the microbial diversity of the rhizosphere was significantly different under the different cultivation methods (P < 0.05). The differences between the microbial species in the rhizosphere of the treated soil were at three class level. The abundance of Alphaproteobacteria and Actinobacteria in the rhizosphere of Avena sativa L. and Vicia sativa L. under the mixed seeding method was conspicuously higher than that of Avena sativa L. and Vicia sativa L. under the clean culture method, while the abundance of Gemmatimonadetes, Nitrospira, and Acidimicrobiia were significantly lower than that obtained under the clean culture method. Regarding fungal predominance, Mortierellomycetes was the most abundant (32.66%) under the mixed seeding method, while the abundance of Sordariomycetes and Leotiomycetes were significantly lower than that under clean culture. The distribution of bacterial and fungal community species in the rhizosphere differed significantly between the treatments. The Kyoto Encyclopedia of Genes and Genomes metabolism analysis showed that the metabolic pathways of functional genes in the soil microbial communities were similar. Conclusions Mixed sowing changed the diversity of plant rhizosphere microbial community structure and promoted plant yield.

scholarly journals Deciphering microbial diversity associated with Fusarium wilt-diseased and disease-free banana rhizosphere soil

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Dengbo Zhou ◽  
Tao Jing ◽  
Yufeng Chen ◽  
Fei Wang ◽  
Dengfeng Qi ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
Fusarium Wilt ◽  
Rhizosphere Soil ◽  
Disease Free

Rhizosphere soil affects pear fruit quality under rain-shelter cultivation

2020 ◽  
Vol 100 (6) ◽  
pp. 683-691
Author(s):  
Xiao-Ming Chen ◽  
Qi Zhang ◽  
Shao-Min Zeng ◽  
Yao Chen ◽  
Yong-Yan Guo ◽  
...  
Keyword(s):  
Carbon Source ◽  
Microbial Diversity ◽  
Open Field ◽  
Fruit Quality ◽  
Rhizosphere Soil ◽  
Soil Enzyme ◽  
Carbon Source Utilization ◽  
Soil Microbial Diversity ◽  
Soil Microbial ◽  
Microbial Carbon

The use of rain shelters in pear cultivation has been shown to improve yields and the appearance and quality of fruit, as well as reduce diseases and pests; however, how rain shelters affect soil chemical properties, soil enzyme activity, and soil microbial diversity remains unknown. Here, we studied pear trees under rain-shelter cultivation and open-field cultivation in the same orchard and compared fruit quality, soil chemical characteristics, soil enzyme activity, and soil microbial diversity. Results showed that rain shelters can significantly (p < 0.05) increase the sugar content (sweetness) of pear fruits and decrease the content of acids. The levels of available phosphorus, available potassium, organic matter, and water in soils under rain shelters were significantly (p < 0.05) lower than in soils in open fields. Rain-shelter treatment increased soil polyphenol oxidase activity and decreased phosphomonoesterase, urease, and sucrase activity. Analysis of microbial carbon-source utilization rates and microbial diversity showed that open-field cultivation is beneficial for microbial carbon-source utilization and microbial diversity in rhizosphere soil. Our study found that rain-shelter cultivation is not beneficial to soil fertility, microbial carbon-source metabolism and utilization, matter cycling, or microbial diversity and that the use of rain shelters may require appropriate nutrient and organic matter supplementation to maintain long-term cultivation of crops; whereas, the effects of environmental factors on open-field cultivation are greater, and more refined water and fertilizer management is required to improve fruit quality.

Rhizospheric soil fungal community patterns of Duchesnea indica in response to altitude gradient in Yunnan, southwest China

2020 ◽  
Vol 66 (5) ◽  
pp. 359-367
Author(s):  
Arslan Jamil ◽  
Jun-Yu Yang ◽  
Dai-Fa Su ◽  
Jiang-Yun Tong ◽  
Shan-Yan Chen ◽  
...  
Keyword(s):  
Community Composition ◽  
Fungal Community ◽  
Altitudinal Gradient ◽  
Rhizosphere Soil ◽  
Fungal Community Composition ◽  
Altitude Gradient ◽  
Community Patterns ◽  
Composition And Structure ◽  
Soil Fungal Community ◽  
Duchesnea Indica

The magnitude of the impact of altitude gradient on microbial community and diversity has been studied in recent decades. Whereas bacteria have been the focus of most studies, fungi have been given relatively less attention. As a vital part of the macro- and microscopic ecosystem, rhizosphere fungi play a key role in organic matter decomposition and relative abundance of plant species and have an impact on plant growth and development. Using Duchesnea indica as the host plant, we examined the rhizosphere soil fungal community patterns across the altitude gradient in 15 sites of Yunnan province by sequencing the fungal ITS2 region with the Illumina MiSeq platform. We determined the fungal community composition and structure. We found that, surprisingly, rhizosphere soil fungal diversity of D. indica increased with altitudinal gradient. There was a slight difference in diversity between samples from high- and medium-altitude sites, with medium-altitude sites having the greater diversity. Furthermore, the rhizosphere soil fungal community composition and structure kept changing along the altitudinal gradient. Taxonomic results showed that the extent of phylum diversity was greatest at high-altitude sites, with Ascomycota, Basidiomycota, Zygomycota, and Glomeromycota as the most dominant fungal phyla.

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