scholarly journals Study on the Microbial Diversity of Rhizosphere Soil of Healthy Tobacco Plants and Plants Infected by Root-knot Nematode

2018 ◽  
Keyword(s):  
Microbial Diversity ◽  
Rhizosphere Soil ◽  
Root Knot Nematode ◽  
Tobacco Plants

scholarly journals A Degeneration Gradient of Poplar Trees Contributes to the Taxonomic, Functional, and Resistome Diversity of Bacterial Communities in Rhizosphere Soils

2021 ◽  
Vol 22 (7) ◽  
pp. 3438
Author(s):  
Juan Liu ◽  
Xiangwei He ◽  
Jingya Sun ◽  
Yuchao Ma
Keyword(s):  
Soil Fertility ◽  
Microbial Diversity ◽  
Resistance Genes ◽  
Bacterial Communities ◽  
Rhizosphere Soil ◽  
Antibiotics Resistance ◽  
Plant Growth Promoting Bacteria ◽  
Soil Microbial Diversity ◽  
Antibiotic Target ◽  
And Function

Bacterial communities associated with roots influence the health and nutrition of the host plant. However, the microbiome discrepancy are not well understood under different healthy conditions. Here, we tested the hypothesis that rhizosphere soil microbial diversity and function varies along a degeneration gradient of poplar, with a focus on plant growth promoting bacteria (PGPB) and antibiotic resistance genes. Comprehensive metagenomic analysis including taxonomic investigation, functional detection, and ARG (antibiotics resistance genes) annotation revealed that available potassium (AK) was correlated with microbial diversity and function. We proposed several microbes, Bradyrhizobium, Sphingomonas, Mesorhizobium, Nocardioides, Variovorax, Gemmatimonadetes, Rhizobacter, Pedosphaera, Candidatus Solibacter, Acidobacterium, and Phenylobacterium, as candidates to reflect the soil fertility and the plant health. The highest abundance of multidrug resistance genes and the four mainly microbial resistance mechanisms (antibiotic efflux, antibiotic target protection, antibiotic target alteration, and antibiotic target replacement) in healthy poplar rhizosphere, corroborated the relationship between soil fertility and microbial activity. This result suggested that healthy rhizosphere soil harbored microbes with a higher capacity and had more complex microbial interaction network to promote plant growing and reduce intracellular levels of antibiotics. Our findings suggested a correlation between the plant degeneration gradient and bacterial communities, and provided insight into the role of high-turnover microbial communities as well as potential PGPB as real-time indicators of forestry soil quality, and demonstrated the inner interaction contributed by the bacterial communities.

scholarly journals Bakteri Endofit asal Tanaman Tembakau sebagai Agens Pengendali Meloidogyne spp.

2019 ◽  
Vol 14 (6) ◽  
pp. 215 ◽  
Author(s):  
Isnainy dinul Mursyalatiyus ◽  
Abdul Munif ◽  
Abdjad Asih Nawangsih
Keyword(s):  
Biological Control ◽  
Endophytic Bacteria ◽  
Biological Control Agent ◽  
Tobacco Plant ◽  
Control Agent ◽  
Root Knot Nematode ◽  
Tobacco Plants ◽  
Physiological Characterization ◽  
Protease Enzyme ◽  
Meloidogyne Spp

Endophytic Bacteria from Tobacco Plant as Biocontrol Agent of  Meloidogyne spp.Soilborne disease on tobacco plants caused by fungal and bacterial infection in association with root-knot nematode (Meloidogyne spp.) may cause significant yield loss.  Endophytic bacteria have been recognized as biological control agent for Meloidogyne spp. as well as  plant promoting growth agent. Research was conducted to evaluate endophytic bacteria isolated from tobacco plants as biological control agent for Meloidogyne spp. infecting tobacco. A total of 215 isolates of endophytic bacteria were isolated from root of two tobacco varieties, Kemloko and Prancak 95.  Biosafety screening showed that 80 isolates (37%) and 7 isolates (8%) gave negative reaction on hypersensitivity test and hemolysis test, respectively.  Seven isolates i.e. TPT3.10, TPT2.1, TK3n8, TK2t21, TK2n8, TK3n1 and TK2t11 were able to promote plant growth and increase the mortality of juvenile Meloidogyne spp. Physiological characterization of endophytic bacteria showed that most of the isolates were able to produce protease enzyme, phosphate, nitrogen and HCN. The same isolates were also able to suppress the number of galls from 80.09% up to 93.82%. Two isolates, TPT3.10 and TK2n8, are considered having the best suppression on root gall formation.

scholarly journals Microbial diversity in rhizosphere soil of soybean grass under different cultivation methods in an alpine region

2020 ◽  
Author(s):  
Ying Zhang ◽  
Wenhui Liu ◽  
Xilai Li ◽  
Zhiying Zhang ◽  
Beibei Su ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microbial Diversity ◽  
Avena Sativa ◽  
Rhizosphere Soil ◽  
Soil Microbial Communities ◽  
Culture Method ◽  
Alpine Region ◽  
Vicia Sativa ◽  
Seeding Method ◽  
Cultivation Methods

Abstract Background A large number of studies have shown that soybean grass with mixed seeding cultivation can significantly improve the yield and quality of forage grass compared with clean culture cultivation.This study explores the differences in the characteristics of the composition and diversity of the microbial community in the rhizosphere of soybean grasses between clean culture and mixed seeding methods in an alpine region. We used high-throughput sequencing technology to determine the microbial diversity and analytical methods to determine the physicochemical characteristics of plant rhizosphere soil of Avena sativa L. and Vicia sativa L. Results There were no significant differences in pH, total nitrogen, total phosphorus, and total potassium in the rhizosphere soil samples of soybean grasses under the clean culture and mixed seeding methods, while there were significant differences in the available nitrogen, available phosphorus, available potassium, and organic matter content (P < 0.05). The bacterial diversity of the rhizosphere soil of Avena sativa L. was the highest under the clean culture method, and the fungal diversity of the rhizosphere soil of Vicia sativa L. was the highest under the clean culture method. Furthermore, the microbial diversity of the rhizosphere was significantly different under the different cultivation methods (P < 0.05). The differences between the microbial species in the rhizosphere of the treated soil were at three class level. The abundance of Alphaproteobacteria and Actinobacteria in the rhizosphere of Avena sativa L. and Vicia sativa L. under the mixed seeding method was conspicuously higher than that of Avena sativa L. and Vicia sativa L. under the clean culture method, while the abundance of Gemmatimonadetes, Nitrospira, and Acidimicrobiia were significantly lower than that obtained under the clean culture method. Regarding fungal predominance, Mortierellomycetes was the most abundant (32.66%) under the mixed seeding method, while the abundance of Sordariomycetes and Leotiomycetes were significantly lower than that under clean culture. The distribution of bacterial and fungal community species in the rhizosphere differed significantly between the treatments. The Kyoto Encyclopedia of Genes and Genomes metabolism analysis showed that the metabolic pathways of functional genes in the soil microbial communities were similar. Conclusions Mixed sowing changed the diversity of plant rhizosphere microbial community structure and promoted plant yield.

scholarly journals Deciphering microbial diversity associated with Fusarium wilt-diseased and disease-free banana rhizosphere soil

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Dengbo Zhou ◽  
Tao Jing ◽  
Yufeng Chen ◽  
Fei Wang ◽  
Dengfeng Qi ◽  
...  
Keyword(s):  
Microbial Diversity ◽  
Fusarium Wilt ◽  
Rhizosphere Soil ◽  
Disease Free

Rhizosphere soil affects pear fruit quality under rain-shelter cultivation

2020 ◽  
Vol 100 (6) ◽  
pp. 683-691
Author(s):  
Xiao-Ming Chen ◽  
Qi Zhang ◽  
Shao-Min Zeng ◽  
Yao Chen ◽  
Yong-Yan Guo ◽  
...  
Keyword(s):  
Carbon Source ◽  
Microbial Diversity ◽  
Open Field ◽  
Fruit Quality ◽  
Rhizosphere Soil ◽  
Soil Enzyme ◽  
Carbon Source Utilization ◽  
Soil Microbial Diversity ◽  
Soil Microbial ◽  
Microbial Carbon

The use of rain shelters in pear cultivation has been shown to improve yields and the appearance and quality of fruit, as well as reduce diseases and pests; however, how rain shelters affect soil chemical properties, soil enzyme activity, and soil microbial diversity remains unknown. Here, we studied pear trees under rain-shelter cultivation and open-field cultivation in the same orchard and compared fruit quality, soil chemical characteristics, soil enzyme activity, and soil microbial diversity. Results showed that rain shelters can significantly (p < 0.05) increase the sugar content (sweetness) of pear fruits and decrease the content of acids. The levels of available phosphorus, available potassium, organic matter, and water in soils under rain shelters were significantly (p < 0.05) lower than in soils in open fields. Rain-shelter treatment increased soil polyphenol oxidase activity and decreased phosphomonoesterase, urease, and sucrase activity. Analysis of microbial carbon-source utilization rates and microbial diversity showed that open-field cultivation is beneficial for microbial carbon-source utilization and microbial diversity in rhizosphere soil. Our study found that rain-shelter cultivation is not beneficial to soil fertility, microbial carbon-source metabolism and utilization, matter cycling, or microbial diversity and that the use of rain shelters may require appropriate nutrient and organic matter supplementation to maintain long-term cultivation of crops; whereas, the effects of environmental factors on open-field cultivation are greater, and more refined water and fertilizer management is required to improve fruit quality.

Microbial Diversity in Tobacco Rhizosphere Soil at Different Growth Stages

2021 ◽  
Vol 15 (5) ◽  
pp. 606-614
Author(s):  
Yanan Ruan ◽  
Shengguang Xu ◽  
Zuoxin Tang ◽  
Xiaolin Liu ◽  
Qirui Zhang ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Microbial Diversity ◽  
Rhizosphere Soil ◽  
Principal Component ◽  
Alpha Diversity ◽  
Illumina Miseq ◽  
Shannon Index ◽  
Growth Stages ◽  
Soil Microbial Diversity ◽  
Different Growth Stages

Rhizosphere microorganisms are the main participants of material transformation and energy cycle in soil. To further explore its composition and variation, the tobacco rhizosphere soil were sequenced by Illumina MiSeq, the microbial community at different growth stages were analyzed and compared. The analysis of Alpha diversity showed that, the Chao1 index, Shannon index of bacteria and Chao1 index of fungi in rhizosphere soil were the highest in tobacco budding stage, while the peak of Shannon index of fungi appeared in tobacco material stage. Principal component analysis (PCA) further showed that at different growth stages, Proteobacteria was the dominant, followed by Actinobacteria, Acidobacteria and Gemmatimonadetes for bacterials; Ascomycota was the dominant, followed by Zygomycota and Basidiomycota for fungi. Under field conditions, the microbial abundance changed with the growth of tobacco, and the microbial diversity reached the peak at budding stage. The bacterial community and abundance between budding and mature stages was highly similar, while the bacterial community in vigorous growth stage is quite different. The similarity of fungal community in budding stage was very low, compared with the other stages; while in other stages was high. This study provides a theoretical basis for further understanding the relationship between tobacco rhizosphere soil microbial diversity and variation, tobacco growth and soil diseases.

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