scholarly journals Isolation and Pathogenic Characterization of Vibrio bivalvicida Associated With a Massive Larval Mortality Event in a Commercial Hatchery of Scallop Argopecten purpuratus in Chile

2019 ◽  
Vol 10 ◽  
Author(s):  
Rodrigo Rojas ◽  
Claudio D. Miranda ◽  
Jaime Romero ◽  
Juan L. Barja ◽  
Javier Dubert
Keyword(s):  
Larval Mortality ◽  
Mortality Event ◽  
Argopecten Purpuratus

De novo assembly, characterization of tissue-specific transcriptomes and identification of immune related genes from the scallop Argopecten purpuratus

2019 ◽  
Vol 89 ◽  
pp. 505-515 ◽  
Author(s):  
Patricio Flores-Herrera ◽  
Rodolfo Farlora ◽  
Roxana González ◽  
Katherina Brokordt ◽  
Paulina Schmitt
Keyword(s):  
De Novo Assembly ◽  
De Novo ◽  
Tissue Specific ◽  
Argopecten Purpuratus ◽  
Immune Related Genes

Molecular Characterization of Primary Juvenile Hormone Responders Methoprene-Tolerant (Met) and Krüppel Homolog 1 (Kr-h1) in Grapholita molesta (Lepidoptera: Tortricidae) with Clarification of Their Roles in Metamorphosis and Reproduction

2019 ◽  
Vol 112 (5) ◽  
pp. 2369-2380 ◽  
Author(s):  
Jing Zhang ◽  
Xiaoxia Liu ◽  
Yichen Liu ◽  
Yueqing An ◽  
Haibo Fang ◽  
...  
Keyword(s):  
Fat Body ◽  
Larval Mortality ◽  
Adult Emergence ◽  
Amino Acid Sequences ◽  
Grapholita Molesta ◽  
Preoviposition Period ◽  
Krüppel Homolog 1 ◽  
Lepidoptera Noctuidae ◽  
Dsrna Injection

AbstractMethoprene-tolerant (Met) is a putative JH intracellular receptor that transduces JH signal by activation of the inducible Krüppel homolog 1 (Kr-h1). We analyzed the gene sequences of Met and Kr-h1 and their patterns of expression in Grapholita molesta (Busck) immature and adult stages in order to better understand the roles of these primary JH responders in regulating the metamorphosis and reproduction of this global pest of fruit crops. The deduced amino acid sequences of both GmMet and GmKr-h1 were highly homologous to those of other Lepidoptera, especially the cotton bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae). Peak expression of GmMet occurred during the last 3 to 5 d of the final instar, followed by that of GmKr-h1, in the last 3 d of final instar. Similar patterns of GmMet and GmKr-h1 expression were detected across various tissue types in the fifth-instar larvae, with the highest expression observed in the head, followed by the epidermis, and the fat body. When expression of GmMet and GmKr-h1 was knocked down via dsRNA injection in the fifth instar, the results were increased larval mortality, abnormal pupation, delayed pupal duration, reduced adult emergence, extended preoviposition period, and reduced fecundity. We infer that both GmMet and GmKr-h1 participated in regulation of metamorphosis and reproduction in G. molesta, the former acting upstream of the latter, and could present biorational targets for novel pest control compounds.

scholarly journals Coupled microbiome analyses highlights relative functional roles of bacteria in a bivalve hatchery

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Emma Timmins-Schiffman ◽  
Samuel J. White ◽  
Rhonda Elliott Thompson ◽  
Brent Vadopalas ◽  
Benoit Eudeline ◽  
...  
Keyword(s):  
Larval Mortality ◽  
Trophic Relationships ◽  
Functional Roles ◽  
Complementary Methods ◽  
Full Characterization ◽  
Host Health ◽  
Pathogenic Microbes ◽  
Species Specific ◽  
Bivalve Hatchery

Abstract Background Microbial communities are ubiquitous throughout ecosystems and are commensal with hosts across taxonomic boundaries. Environmental and species-specific microbiomes are instrumental in maintaining ecosystem and host health, respectively. The introduction of pathogenic microbes that shift microbiome community structure can lead to illness and death. Understanding the dynamics of microbiomes across a diversity of environments and hosts will help us to better understand which taxa forecast survival and which forecast mortality events. Results We characterized the bacterial community microbiome in the water of a commercial shellfish hatchery in Washington state, USA, where the hatchery has been plagued by recurring and unexplained larval mortality events. By applying the complementary methods of metagenomics and metaproteomics we were able to more fully characterize the bacterial taxa in the hatchery at high (pH 8.2) and low (pH 7.1) pH that were metabolically active versus present but not contributing metabolically. There were shifts in the taxonomy and functional profile of the microbiome between pH and over time. Based on detected metagenomic reads and metaproteomic peptide spectral matches, some taxa were more metabolically active than expected based on presence alone (Deltaproteobacteria, Alphaproteobacteria) and some were less metabolically active than expected (e.g., Betaproteobacteria, Cytophagia). There was little correlation between potential and realized metabolic function based on Gene Ontology analysis of detected genes and peptides. Conclusion The complementary methods of metagenomics and metaproteomics contribute to a more full characterization of bacterial taxa that are potentially active versus truly metabolically active and thus impact water quality and inter-trophic relationships.

scholarly journals Co-infection by alveolate parasites and frog virus 3-like ranavirus during an amphibian larval mortality event in Florida, USA

2013 ◽  
Vol 105 (2) ◽  
pp. 89-99 ◽  
Author(s):  
JH Landsberg ◽  
Y Kiryu ◽  
M Tabuchi ◽  
TB Waltzek ◽  
KM Enge ◽  
...  
Keyword(s):  
Larval Mortality ◽  
Frog Virus 3 ◽  
Frog Virus ◽  
Mortality Event

Synthesis, characterization of metal complexes of dehydroacetic-1,8-naphthaleimine. Study their effect on the larval mortality of Parasarcophaga aegyptiaca salem

2013 ◽  
Vol 12 (3) ◽  
pp. 209-220 ◽  
Author(s):  
Abdou S. El-Tabl ◽  
Ramadan M. El-Bahnasawy ◽  
Mohamad M.E. Shakdofa ◽  
Ahemd M.A. El-Seidy ◽  
Mohamed F. Faramawy
Keyword(s):  
Metal Complexes ◽  
Larval Mortality
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