scholarly journals Vector Competence of Thrips Species to Transmit Soybean Vein Necrosis Virus

2019 ◽  
Vol 10 ◽  
Author(s):  
Jinlong Han ◽  
Vamsi J. Nalam ◽  
I-Chen Yu ◽  
Punya Nachappa
Keyword(s):  
Vector Competence ◽  
Necrosis Virus ◽  
Thrips Species ◽  
Vein Necrosis

Molecular characterization and incidence of new tospovirus: Soybean Vein Necrosis Virus (SVNV) in Egypt

2024 ◽  
Vol 84 ◽  
Author(s):  
A. S. Abd El-Wahab
Keyword(s):  
Plant Species ◽  
Abundant Species ◽  
High Rate ◽  
Survey Study ◽  
Mechanical Transmission ◽  
Necrosis Virus ◽  
Thrips Species ◽  
Vein Necrosis ◽  
Soybean Plants ◽  
Natural Incidence

Abstract Field survey study was conducted season (2017). Soybeans and weeds were weekly sampled randomly. Thrips adults were identified and counted. Detection of the virus isolate and the natural incidence was determined using; Mechanical transmission, host range, DAS-ELISA, RT-PCR. The natural incidence thrips individuals was detected depending on the SVNV% in thrips individuals and weeds hosts. Ten thrips species were associated with soybean plants in the field. The most abundant species was T. tabaci, average 256.5 average no.of individuals, followed by F. occidentalis (142.5 average no. of individuals), then N. variabilis (86.6/ average no. of individuals). Fourteen thrips species occurred on 5 legumes field crops and 41 weed plant species within soybean field. The highest average number 40.6.of individuals were recorded on Ammi majus. While the lowest one 3.3 average no. of individuals were on Urtica urens. Only 21diagnostic plant species were susceptible to infection with SVNV. G. max and Vigna radiate, were the highest percentage of infection 80% followed by V. unguilata & N. benthamiana, 75%. Egyptian isolate of Soybean vein necrosis virus (SVNV) in this study showed a high degree of similarity and it is closely related to TSWV from Egypt (DQ479968) and TCSV from USA (KY820965) with nucleotide sequence identity of 78%. Four thrips species transmitted SVNV (F. fusca 4.0%, F. schultzei 4.3%, F. tritici 3.3% and N. variabilis 68.0% transmission). Both C. phaseoli and M. sjostedti can acquire the virus but unable to transmit it. The following species; T. tabaci, F. occidentalis, S. dorsallis and T. palmi cannot acquire or transmit SVNV. The incidence of SVNV in the field started by the end of July then increased gradualy from 12.7 to 71.3% by the end of the season. In conclusion, few thrips individuals invaded soybean crops are enough to transmit high rate of SVNV within the crop. Furthermore, several vector species are also abundant on weeds, which are the major sources of soybean viruses transmitted to the crops. This information might be important for control and reduce the incidence of SVNV infection.

scholarly journals Progression of Watermelon Bud Necrosis Virus Infection in Its Vector, Thrips palmi

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 392
Author(s):  
Amalendu Ghosh ◽  
Priti ◽  
Bikash Mandal ◽  
Ralf G. Dietzgen
Keyword(s):  
Virus Infection ◽  
Salivary Glands ◽  
Frankliniella Occidentalis ◽  
Western Flower Thrips ◽  
Malpighian Tubules ◽  
Necrosis Virus ◽  
Posterior Portion ◽  
Thrips Palmi ◽  
Thrips Species ◽  
Anterior Midgut

Thrips are important pests of agricultural, horticultural, and forest crops worldwide. In addition to direct damages caused by feeding, several thrips species can transmit diverse tospoviruses. The present understanding of thrips–tospovirus relationships is largely based on studies of tomato spotted wilt virus (TSWV) and Western flower thrips (Frankliniella occidentalis). Little is known about other predominant tospoviruses and their thrips vectors. In this study, we report the progression of watermelon bud necrosis virus (WBNV) infection in its vector, melon thrips (Thrips palmi). Virus infection was visualized in different life stages of thrips using WBNV-nucleocapsid protein antibodies detected with FITC-conjugated secondary antibodies. The anterior midgut was the first to be infected with WBNV in the first instar larvae. The midgut of T. palmi was connected to the principal salivary glands (PSG) via ligaments and the tubular salivary glands (TSG). The infection progressed to the PSG primarily through the connecting ligaments during early larval instars. The TSG may also have an ancillary role in disseminating WBNV from the midgut to PSG in older instars of T. palmi. Infection of WBNV was also spread to the Malpighian tubules, hindgut, and posterior portion of the foregut during the adult stage. Maximum virus-specific fluorescence in the anterior midgut and PSG indicated the primary sites for WBNV replication. These findings will help to better understand the thrips–tospovirus molecular relationships and identify novel potential targets for their management. To our knowledge, this is the first report of the WBNV dissemination path in its vector, T. palmi.

Soybean vein necrosis virus: an emerging virus in North America

Virus Genes ◽  
2018 ◽  
Vol 55 (1) ◽  
pp. 12-21 ◽  
Author(s):  
Jing Zhou ◽  
Ioannis E. Tzanetakis
Keyword(s):  
North America ◽  
Necrosis Virus ◽  
Emerging Virus ◽  
Vein Necrosis

scholarly journals Evaluation of Soybean for Resistance to Neohyadatothrips variabilis (Thysanoptera: Thripidae) Noninfected and Infected With Soybean Vein Necrosis Virus

2019 ◽  
Vol 113 (2) ◽  
pp. 949-955 ◽  
Author(s):  
D Lagos-Kutz ◽  
M L Pawlowski ◽  
J Haudenshield ◽  
J Han ◽  
L L Domier ◽  
...  
Keyword(s):  
Choice Experiments ◽  
The United States ◽  
Necrosis Virus ◽  
Plant Introductions ◽  
Soybean Genotypes ◽  
Efficient Vector ◽  
Vein Necrosis ◽  
Neohydatothrips Variabilis ◽  
Multiple Species ◽  
Soybean Thrips

Abstract Soybean vein necrosis virus (SVNV) was first identified in Arkansas and Tennessee in 2008 and is now known to be widespread in the United States and Canada. Multiple species of thrips transmit this and other tospoviruses with Neohydatothrips variabilis (Beach) (soybean thrips) cited as the most efficient vector for SVNV. In this study, 18 soybean, Glycine max (L.) Merr., genotypes were evaluated in four experiments by infesting plants with noninfected and SVNV-infected thrips using choice and no-choice assays. In both choice experiments with noninfected and SVNV-infected thrips, the lowest number of immature soybean thrips occurred on plant introductions (PIs) 229358 and 604464 while cultivars Williams 82 and Williamsfield Illini 3590N supported higher counts of mature thrips. The counts between the two assays (noninfected and SVNV-infected thrips) were positively correlated. In both no-choice experiments with noninfected and SVNV-infected thrips, counts of thrips did not differ by soybean genotypes. Further studies are needed to characterize the inheritance and mechanisms involved in the resistance found in the choice assay.

scholarly journals First Report of Soybean vein necrosis virus in Soybean Fields of Oklahoma

Plant Disease ◽  
2013 ◽  
Vol 97 (12) ◽  
pp. 1664-1664 ◽  
Author(s):  
A. Ali ◽  
O. A. Abdalla
Keyword(s):  
Consensus Sequence ◽  
The United States ◽  
Necrosis Virus ◽  
Potential Threat ◽  
First Report ◽  
Leaf Vein ◽  
Three Samples ◽  
Vein Necrosis ◽  
Soybean Plants ◽  
Seed Crops

Soybean vein necrosis virus (SVNV) causes a new emerging disease of soybean that has been recorded in more than 10 states (1,2,3,4) of the United States, but so far no information is available about its presence in soybean crops of Oklahoma. Surveys of commercial soybean fields were conducted for soybean viruses during summer of 2012. A total of 327 samples were randomly collected from soybean fields in 11 counties. Symptoms typical of SVNV infections including leaf chlorosis and leaf-vein necrosis were observed on some soybean plants in the field (4). All soybean leaf samples were tested against SVNV polyclonal antisera obtained from AC Diagnostics, Inc. (Fayetteville, AR) by dot-immunobinding assay (DIBA) (1). Fifty-three samples reacted positively with SVNV antisera. Total RNA was extracted from three DIBA-positive samples collected from soybean plants in Choctaw County and tested by reverse transcription (RT)-PCR using SVNV-specific primers (forward primer 5′-ATGTTCTCTCTATAATAGCCA and reverse primer 5′-ACCCATAACAATTGATCAAGA-3′) that were designed from the available sequence in the GenBank (Accession No. GU722317.1) to amplify a fragment from RNA1. A band of the expected size of 344 bp was observed on a 1% agarose gel in all three samples. The PCR products were purified using QIAquick PCR Purification Kit (QIAGEN, Valencia, CA), cloned (pGEM-T Easy Vector, Promega, Madison, WI) and sequenced in both directions. The consensus sequence of the 344-bp fragment was 99% identical with the corresponding region of RNA 1 of SVNV isolate ‘Milan_TN’ (Accession No. GU722317.1). These results confirmed the presence of SVNV in soybean fields, which are mostly located in Criage, Choctaw, Hughes, LeFlore, Mayes, Muskogee, McCurtain, Okmulgee, Ottawa, Rogers, and Sequoyah counties of Oklahoma. None of the samples collected from north central or western parts of the state were positive against SVNV. To our knowledge, this is the first report of SVNV in soybean crops in Oklahoma. Soybean is one of the major oil seed crops cultivated on approximately 200,000 hectares annually in Oklahoma and the presence of SVNV could pose a potential threat to the production of soybean in the future. References: (1) J. L. Jacobs and M. I. Chilvers. Plant Dis. 97:1387, 2013. (2) J. Han et al. Plant Dis. 97:693, 2013. (3) D. L. Smith et al. Plant Dis. 97:693, 2013. (4) J. Zhou et al. Virus Genes 43:289, 2011.

scholarly journals Ceratothripoides claratris, a New Vector of a Capsicum chlorosis virus Isolate Infecting Tomato in Thailand

2005 ◽  
Vol 95 (6) ◽  
pp. 659-663 ◽  
Author(s):  
W. T. S. D. Premachandra ◽  
C. Borgemeister ◽  
E. Maiss ◽  
D. Knierim ◽  
H.-M. Poehling
Keyword(s):  
Larval Stage ◽  
Vector Competence ◽  
Close Association ◽  
Transmission Efficiency ◽  
Adult Males ◽  
Tomato Plants ◽  
First Instar ◽  
Thrips Species ◽  
Infected Tomato ◽  
Capsicum Chlorosis Virus

Ceratothripoides claratris, the predominant thrips species on tomato in Thailand, was tested for vector competence and efficiency to transmit Capsicum chlorosis virus (CaCV) (isolate AIT) to tomato. The efficiency of adult-stage transmission was influenced by the larval stage at which virus was acquired. Adult C. claratris showed 69% transmission efficiency after acquiring the virus as freshly emerged (<1 h) first-instar larvae. However, when just molted (<1 h) second-instar larvae acquired the virus, the percentage of adult transmitters significantly decreased (48%). Transmission efficiency of up to 47% was detected with second-instar larvae of C. claratris which had acquired the virus as freshly emerged first-instar larvae. Transmission efficiency did not significantly differ between adult males and females, irrespective of the larval stage at which the virus was acquired. Highest transmission efficiency for CaCV was recorded in adult C. claratris derived from second-instar larvae collected from infected tomato plants in a greenhouse. Lowest transmission efficiency was observed in adults directly collected from infected tomato plants in the greenhouse. The spread of CaCV on tomato plants in greenhouses showed a close association with thrips infestations.

Stipple streak disease of French bean caused by a tobacco necrosis virus in Queensland

1968 ◽  
Vol 19 (5) ◽  
pp. 731 ◽  
Author(s):  
GM Behncken
Keyword(s):  
French Bean ◽  
Tobacco Necrosis Virus ◽  
Serological Evidence ◽  
Necrosis Virus ◽  
Leaf Vein ◽  
Satellite Virus ◽  
Vein Necrosis ◽  
Stem Necrosis ◽  
Olpidium Brassicae

A disease of beans in the Nambour district of Queensland has been shown to be stipple streak disease caused by a tobacco necrosis virus. Symptoms include leaf vein necrosis, stem necrosis, and occasionally necrotic lesions on the pods. In glasshouse tests symptoms developed more rapidly, and were more severe, at temperatures of 80–88°F than at 62–70°. The virus was readily transmitted by zoospores of a lettuce isolate of the fungus Olpidium brassicae (Wor.) Dang. Serological evidence is presented which indicates that the virus is an "A" serotype strain of tobacco necrosis virus. No evidence for the presence of an associated satellite virus was found.

Complete nucleotide sequence of an isolate of coleus vein necrosis virus from verbena

2007 ◽  
Vol 153 (2) ◽  
pp. 381-384 ◽  
Author(s):  
J. Kraus ◽  
I. E. Tzanetakis ◽  
M. L. Putnam ◽  
R. R. Martin
Keyword(s):  
Nucleotide Sequence ◽  
Complete Nucleotide Sequence ◽  
Necrosis Virus ◽  
Vein Necrosis
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