scholarly journals Overview of the Antimicrobial Compounds Produced by Members of the Bacillus subtilis Group

2019 ◽  
Vol 10 ◽  
Author(s):  
Simon Caulier ◽  
Catherine Nannan ◽  
Annika Gillis ◽  
Florent Licciardi ◽  
Claude Bragard ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Antimicrobial Compounds ◽  
Subtilis Group

ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT

2009 ◽  
Vol 1 (2) ◽  
Author(s):  
Risa Nofiani ◽  
Siti Nurbetty ◽  
Ajuk Sapar
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Agar Medium ◽  
Antimicrobial Activities ◽  
Antimicrobial Compounds ◽  
Associated Bacteria ◽  
Minimum Inhibitory Concentrations ◽  
Sponge Associated Bacteria

<p>The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 μg/well for S. aureus, 950 μg/well for Salmonella sp.and 800 μg/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 μg/well for S. aureus, 1,050 μg/well for Salmonella sp., 750 μg/well for Bacillus subtilis, 350 μg/well for P. aeruginosa, 750 μg/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity.</p> <p>Keywords:Antimicrobial,Sponges associated bacteria,MICs</p>

scholarly journals The Change of Bacterial Spectrum after Storage of X. campestris pv. campestris Inoculated Cabbage Heads (Brassica oleracea var. capitata L.)

Agronomy ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 443 ◽  
Author(s):  
Lucia Ragasová ◽  
Eliška Peňázová ◽  
Filip Gazdík ◽  
Jakub Pečenka ◽  
Jana Čechová ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Brassica Oleracea ◽  
Storage Conditions ◽  
Bacillus Species ◽  
Pseudomonas Sp ◽  
Bacterial Cultures ◽  
Bacterial Spectrum ◽  
Minor Part ◽  
Cruciferous Plants ◽  
Subtilis Group

Changes in the bacterial spectrum of cabbage heads after storage under commonly used storage conditions were examined in this study. Cabbage seeds (Brassica oleracea var. capitata L.) were artificially inoculated with X. campestris pv. campestris (Xcc), a serious pathogen of cruciferous plants causing black rot. Isolation of bacterial cultures from Xcc-inoculated and non-inoculated cabbage heads were carried out in two time points—at the day of harvest and after four months of storage. According to our previous research and literature reports, the most frequent genera of bacteria were chosen for PCR testing, i.e., Bacillus cereus group, Bacillus subtilis group, Pseudomonas sp., and X. campestris pv. campestris. A few of the obtained bacterial cultures were negative for the four above-mentioned species. In those, other bacteria were identified by 16S rRNA sequencing. In both Xcc-inoculated and non-inoculated cabbage heads, changes of the bacterial spectrum over time were observed. The severity of Xcc infection of heads increased after four months of storage. Bacillus species represented the most frequently occurring bacterial genus. The presence of the Bacillus subtilis group increased significantly after storage in non-inoculated cabbage heads. The minor part of the other genera identified by sequencing in the first sampling were not detected in the stored cabbage heads. This was associated with a possible antagonistic behavior of Pseudomonas sp. and Bacillus sp.

Raman microspectroscopy as an identification tool within the phylogenetically homogeneous ‘Bacillus subtilis’-group

2006 ◽  
Vol 29 (8) ◽  
pp. 650-660 ◽  
Author(s):  
Didier Hutsebaut ◽  
Joachim Vandroemme ◽  
Jeroen Heyrman ◽  
Peter Dawyndt ◽  
Peter Vandenabeele ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Raman Microspectroscopy ◽  
Subtilis Group ◽  
Identification Tool

Two distinct groups within the Bacillus subtilis group display significantly different spore heat resistance properties

2015 ◽  
Vol 45 ◽  
pp. 18-25 ◽  
Author(s):  
Erwin M. Berendsen ◽  
Marcel H. Zwietering ◽  
Oscar P. Kuipers ◽  
Marjon H.J. Wells-Bennik
Keyword(s):  
Bacillus Subtilis ◽  
Heat Resistance ◽  
Subtilis Group

scholarly journals THE SOURCE OF THE MICROORGANISMS IN THE LUNGS OF NORMAL ANIMALS

1922 ◽  
Vol 36 (3) ◽  
pp. 317-328 ◽  
Author(s):  
F. S. Jones
Keyword(s):  
Bacillus Subtilis ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
White Mouse ◽  
Lymph Glands ◽  
Dry Food ◽  
Subtilis Group

It has been possible to show that the lungs of such animals as the calf, rabbit, guinea pig, white rat, and white mouse are readily invaded by organisms. The most frequent types observed in cultures from the border of the lungs have been streptothrix, molds, and bacteria of the Bacillus subtilis group. These forms originate in certain dry food stuffs (hay and straw). By withholding or moistening these materials it has been possible to diminish the number of organisms in the lungs. When these materials have been supplied to mice whose lungs under usual conditions contain only a few organisms, the number of positive cultures increases and is comparable with those of the larger animals. The bronchial lymph glands of all guinea pigs examined developed, in 66⅔ per cent of the tubes, organisms similar to those obtained from the lungs.

scholarly journals Complete Genome Sequence of Bacillus subtilis J-5, a Potential Biocontrol Agent

2017 ◽  
Vol 5 (23) ◽  
Author(s):  
Zhenhua Jia ◽  
Weiwei Jin ◽  
Yali Huang ◽  
Shuishan Song
Keyword(s):  
Bacillus Subtilis ◽  
Biocontrol Agent ◽  
Rhizosphere Soil ◽  
Whole Genome ◽  
Antimicrobial Compounds ◽  
Content Type ◽  
Regulatory Systems ◽  
Potential Biocontrol Agent ◽  
Genes Encoding ◽  
Shed Light

ABSTRACT Bacillus subtilis J-5 was isolated from tomato rhizosphere soil and exhibited strong inhibitory activity against Botrytis cinerea. To shed light on the molecular mechanism underlying the biological control on phytopathogens, the whole genome of this strain was sequenced. Genes encoding antimicrobial compounds and the regulatory systems were identified in the genome.

scholarly journals Panophthalmitis due to an Organism of the Bacillus Subtilis Group

1952 ◽  
Vol 36 (7) ◽  
pp. 389-392 ◽  
Author(s):  
R. Davenport ◽  
C. Smith
Keyword(s):  
Bacillus Subtilis ◽  
Subtilis Group

scholarly journals Optimization of medium composition for the production of compounds effective against Xanthomonas campestris by bacillus subtilis

2014 ◽  
pp. 247-258 ◽  
Author(s):  
Zorana Roncevic ◽  
Jovana Grahovac ◽  
Damjan Vucurovic ◽  
Sinisa Dodic ◽  
Bojana Bajic ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Sodium Nitrite ◽  
Xanthomonas Campestris ◽  
Inhibition Zone ◽  
Medium Composition ◽  
Plant Diseases ◽  
Antimicrobial Compounds ◽  
Promising Alternative ◽  
Inhibition Zone Diameter ◽  
Zone Diameter

The biocontrol agents are a very promising alternative to synthetic pesticides that are presently used to control plant diseases caused by phytopathogenic microorganisms. Members of the Bacillus genera are soil bacteria that produce significant quantities of agriculturally important bioactive compounds. Production of these compounds can be improved by changing the nutritional and environmental conditions. The aim of this study was the optimization of medium composition, using response surface methodology, for the production of compounds effective against Xanthomonas campestris ATCC 13951 by Bacillus subtilis ATCC 6633. To study the production of antimicrobial compounds by selected Bacillus strain, the producing microorganisms were cultivated on nutrient broth. The inhibition zone diameter of 18.0 mm obtained by the diffusion-disc method indicated that the used Bacillus subtilis strain produces compounds with antimicrobial activity against Xanthomonas campestris ATCC 13951. To optimize the composition of the cultivation medium in terms of glycerol, sodium nitrite and phosphates content, experiments were carried out in accordance with Box-Behnken design, and optimization of multiple responses was performed using the concept of desirability function. The developed model predicted that the maximum inhibition zone diameter (26.23 mm) against tested phytopathogen is achieved when the initial content of glycerol, sodium nitrite and phosphate were 50.00 g/L, 2.85 g/L and 11.00 g/L, respectively. To minimize the consumption of medium components and costs of effluents processing, additional optimization set was made. The techno-economic analysis of the obtained results has to be done to select optimal medium composition for industrial production of antimicrobial compounds.

ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT

2009 ◽  
Vol 1 (2) ◽  
Author(s):  
Risa Nofiani ◽  
Siti Nurbetty ◽  
Ajuk Sapar
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Agar Medium ◽  
Antimicrobial Activities ◽  
Antimicrobial Compounds ◽  
Associated Bacteria ◽  
Minimum Inhibitory Concentrations ◽  
Sponge Associated Bacteria

The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 μg/well for S. aureus, 950 μg/well for Salmonella sp.and 800 μg/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 μg/well for S. aureus, 1,050 μg/well for Salmonella sp., 750 μg/well for Bacillus subtilis, 350 μg/well for P. aeruginosa, 750 μg/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity. Keywords:Antimicrobial,Sponges associated bacteria,MICs

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