scholarly journals Stable isotope labelling: an approach for MALDI-TOF MS-based rapid detection of fluconazole resistance in Candida tropicalis

2019 ◽  
Vol 74 (5) ◽  
pp. 1269-1276 ◽  
Author(s):  
Saikat Paul ◽  
Shreya Singh ◽  
Arunaloke Chakrabarti ◽  
Shivaprakash M Rudramurthy ◽  
Anup K Ghosh
Keyword(s):  
Stable Isotope ◽  
Candida Tropicalis ◽  
Rapid Detection ◽  
Stable Isotope Labelling ◽  
Fluconazole Resistance ◽  
Isotope Labelling ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Rapid detection of fluconazole resistance in Candida tropicalis by MALDI-TOF MS

2017 ◽  
Vol 56 (2) ◽  
pp. 234-241 ◽  
Author(s):  
Saikat Paul ◽  
Pankaj Singh ◽  
Shamanth A S ◽  
Shivaprakash M Rudramurthy ◽  
Arunaloke Chakrabarti ◽  
...  
Keyword(s):  
Candida Tropicalis ◽  
Rapid Detection ◽  
Fluconazole Resistance ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals RAPID DETECTION OF FLUCONAZOLE RESISTANCE IN CANDIDA TROPICALIS BY MALDI-TOF-MS

2019 ◽  
Vol 3 (3) ◽  
Author(s):  
Shivi Saxena ◽  
Rupal Sengar
Keyword(s):  
Candida Tropicalis ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Fluconazole Resistance ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Matrix Assisted Laser

The study of disease transmission and development of antifungal opposition among Candida species, rapid antifungal susceptibility testing (AFST) is essential for streamlining of antifungal treatment. This investigation was directed to institutionalize a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI - TOF MS) based AFST strategy (ms- AFST) for helplessness of Candida tropicalis detaches. Clinical secludes of C. tropicalis were affirmed for fluconazole obstruction by the CLSI (M27-A3) strategy. The incubation period and medication concentration were advanced to decide the insignificant profile change concentration (MPCC) by MALDI-TOF MS. The information were broke down first by direct visual  perception of the spectra pursued by composite connection list (CCI) matrix investigation,  virtual gel investigation, and group examination for affirmation. At long last, the connection between's minimum inhibitory concentrations (MICs) and MPCCs was assessed. A sum of 14 fluconazole resistance (MICs extending from 15 to 127μg/ml) and 18 fluconazole subseptible C.  tropicalis segregates (MIC ≤1μg/ml) were incorporated into this examination. All C. tropicalis separates had noteworthy otherworldly changes after 4h concentration with fluconazole. Of 33 confines, MPCCs what's more, MICs were equal for 15 disengages, and the MPCC was one weakening lower than the particular MIC in the rest of the 17 secludes. This finding was additionally upheld by visual investigation, CCI framework examination, virtual gel and chief part examination dendrogram investigation. The relationship among's MPCC and MIC was huge (P <.05). In this way, a MALDI-TOF MS based AFST examine might be utilized as a fast screening procedure for fluconazole against in C. tropicalis. Key words: matrix assisted laser desorption ionization-time of flight mass spectrometry, composite correlation index, principal component analysis, Candida tropicalis.

Rapid detection and identification of strains carrying carbapenemases directly from positive blood cultures using MALDI-TOF MS

2014 ◽  
Vol 105 ◽  
pp. 98-101 ◽  
Author(s):  
Y. Hoyos-Mallecot ◽  
C. Riazzo ◽  
C. Miranda-Casas ◽  
M.D. Rojo-Martín ◽  
J. Gutiérrez-Fernández ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Blood Cultures ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Detection And Identification ◽  
Tof Ms

Rapid detection of Lactococcus lactis isolates producing the lantibiotics nisin, lacticin 481 and lacticin 3147 using MALDI-TOF MS

2017 ◽  
Vol 139 ◽  
pp. 138-142 ◽  
Author(s):  
Tomás García-Cayuela ◽  
Teresa Requena ◽  
M. Carmen Martínez-Cuesta ◽  
Carmen Peláez
Keyword(s):  
Lactococcus Lactis ◽  
Rapid Detection ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Lacticin 3147 ◽  
Lacticin 481 ◽  
Tof Ms

scholarly journals Rapid detection of GM1 ganglioside in cerebrospinal fluid in dogs with GM1 gangliosidosis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry

2011 ◽  
Vol 23 (6) ◽  
pp. 1202-1207 ◽  
Author(s):  
Hiroyuki Satoh ◽  
Toyofumi Yamauchi ◽  
Masahiro Yamasaki ◽  
Yoshimitsu Maede ◽  
Akira Yabuki ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Rapid Detection ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Gm1 Gangliosidosis ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

The concentration of GM1 (monosialotetrahexosyl ganglioside) in cerebrospinal fluid (CSF) is markedly increased in dogs with GM1 gangliosidosis due to GM1 accumulation in the central nervous system and leakage to the CSF. The present study established a rapid and simple method for detection of accumulated GM1 in the CSF in dogs with GM1 gangliosidosis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) and discusses the usefulness of this method for the rapid diagnosis and/or high-risk screening of this disease in domestic animals. Cerebrospinal fluid was collected from normal dogs and 4- to 11-month-old Shiba dogs with GM1 gangliosidosis. The MALDI TOF MS analysis was carried out in combination with a special sample plate and a simple desalting step on the plate. Specific signs of GM1 could be detected in the standard GM1 solutions at concentrations of 50 nmol/l or more. The signs were also clearly detected in CSF (131–618 nmol/l) in affected dogs, but not in normal canine CSF (12 ± 5 nmol/l, mean ± standard deviation). The results demonstrated that MALDI TOF MS can detect GM1 accumulated in canine CSF even in the early stage of the disease. In conclusion, the rapid detection of increased CSF GM1 using MALDI TOF MS is a useful method for diagnosis and/or screening for canine GM1 gangliosidosis.

MALDI-TOF-MS integrated workflow for food authenticity investigations: An untargeted protein-based approach for rapid detection of PDO feta cheese adulteration

2021 ◽  
pp. 131057
Author(s):  
Anastasia S. Kritikou ◽  
Reza Aalizadeh ◽  
Dimitrios E. Damalas ◽  
Ioanna V. Barla ◽  
Carsten Baessmann ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Food Authenticity ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Feta Cheese ◽  
Tof Ms
Sign in / Sign up

Export Citation Format

Share Document