scholarly journals Antimicrobial Activity of NCR Plant Peptides Strongly Depends on the Test Assays

2018 ◽  
Vol 9 ◽  
Author(s):  
Attila Farkas ◽  
Bernadett Pap ◽  
Éva Kondorosi ◽  
Gergely Maróti
Keyword(s):  
Antimicrobial Activity ◽  
Plant Peptides

scholarly journals Comparative Analysis of the Antimicrobial Activities of Plant Defensin-Like and Ultrashort Peptides against Food-Spoiling Bacteria

2016 ◽  
Vol 82 (14) ◽  
pp. 4288-4298 ◽  
Author(s):  
Joanna Kraszewska ◽  
Michael C. Beckett ◽  
Tharappel C. James ◽  
Ursula Bond
Keyword(s):  
Antimicrobial Activity ◽  
Food Poisoning ◽  
Structural Similarity ◽  
Antimicrobial Activities ◽  
Human Society ◽  
Plant Defensin ◽  
Food Spoilage ◽  
Economic Implications ◽  
Wide Range ◽  
Plant Peptides

ABSTRACTAntimicrobial peptides offer potential as novel therapeutics to combat food spoilage and poisoning caused by pathogenic and nonpathogenic bacteria. Our previous studies identified the peptide human beta-defensin 3 (HBD3) as a potent antimicrobial agent against a wide range of beer-spoiling bacteria. Thus, HBD3 is an excellent candidate for development as an additive to prevent food and beverage spoilage. To expand the repertoire of peptides with antimicrobial activity against bacteria associated with food spoilage and/or food poisoning, we carried out anin silicodiscovery pipeline to identify peptides with structure and activity similar to those of HBD3, focusing on peptides of plant origin. Using a standardized assay, we compared the antimicrobial activities of nine defensin-like plant peptides to the activity of HBD3. Only two of the peptides, fabatin-2 and Cp-thionin-2, displayed antimicrobial activity; however, the peptides differed from HBD3 in being sensitive to salt and were thermostable. We also compared the activities of several ultrashort peptides to that of HBD3. One of the peptides, the synthetic tetrapeptide O3TR, displayed biphasic antimicrobial activity but had a narrower host range than HBD3. Finally, to determine if the peptides might act in concert to improve antimicrobial activity, we compared the activities of the peptides in pairwise combinations. The plant defensin-like peptides fabatin-2 and Cp-thionin-2 displayed a synergistic effect with HBD3, while O3TR was antagonistic. Thus, some plant defensin-like peptides are effective antimicrobials and may act in concert with HBD3 to control bacteria associated with food spoilage and food poisoning.IMPORTANCEFood spoilage and food poisoning caused by bacteria can have major health and economic implications for human society. With the rise in resistance to conventional antibiotics, there is a need to identify new antimicrobials to combat these outbreaks in our food supply. Here we screened plant peptide databases to identify peptides that share structural similarity with the human defensin peptide HBD3, which has known antimicrobial activity against food-spoiling bacteria. We show that two of the plant peptides display antimicrobial activity against bacteria associated with food spoilage. When combined with HBD3, the peptides are highly effective. We also analyzed the activity of an easily made ultrashort synthetic peptide, O3TR. We show that this small peptide also displays antimicrobial activity against food-spoiling bacteria but is not as effective as HBD3 or the plant peptides. The plant peptides identified are good candidates for development as natural additives to prevent food spoilage.

Antimicrobial activity of withanolides from the leaves of Withania aristata

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
L Araujo ◽  
N Padilla ◽  
GG Llanos ◽  
IL Bazzocchi ◽  
L Moujir
Keyword(s):  
Antimicrobial Activity

Antimicrobial activity of Eurycoma longifolia and Momordica charantia

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
R Łos ◽  
K Skalicka-Wozniak ◽  
J Widelski ◽  
A Malm ◽  
K Głowniak
Keyword(s):  
Antimicrobial Activity ◽  
Momordica Charantia ◽  
Eurycoma Longifolia

Antioxidant and antimicrobial activity of willow herb (Epilobium angustifolium L.)

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
I Kosalec ◽  
M Zovko ◽  
K Sankovic ◽  
D Kremer ◽  
S Pepeljnjak
Keyword(s):  
Antimicrobial Activity ◽  
Epilobium Angustifolium ◽  
Antioxidant And Antimicrobial Activity

Cytotoxicity, antimicrobial activity and composition of essential oil from Tanacetum balsamita L.subsp. balsamita

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
SN Ebrahimi ◽  
M Yousefzadi ◽  
A Sonboli ◽  
F Miraghasi ◽  
S Ghiasi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil

Chemical composition and antimicrobial activity of the essential oils of flowers, leaves and stems of Cotinus coggygria

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
T Milošević ◽  
N Nićiforović ◽  
V Mihailović ◽  
S Solujić ◽  
N Vuković
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Cotinus Coggygria

Selective antimicrobial activity of biochanin A

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
J Flesar ◽  
O Sklenickova ◽  
E Vlkova ◽  
J Malik ◽  
L Kokoska
Keyword(s):  
Antimicrobial Activity ◽  
Biochanin A ◽  
Selective Antimicrobial Activity
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