scholarly journals Bacterial Abundance and Community Composition in Pond Water From Shrimp Aquaculture Systems With Different Stocking Densities

2018 ◽  
Vol 9 ◽  
Author(s):  
Yustian Rovi Alfiansah ◽  
Christiane Hassenrück ◽  
Andreas Kunzmann ◽  
Arief Taslihan ◽  
Jens Harder ◽  
...  
Keyword(s):  
Community Composition ◽  
Bacterial Abundance ◽  
Pond Water ◽  
Shrimp Aquaculture

scholarly journals Bacterial abundance and community composition in green, brown and red water from intensive catfish (Clarias sp.) culture ponds in Yogyakarta, Indonesia

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
Karunia Adetera Nungki Wijayanti ◽  
Indah Istiqomah ◽  
Murwantoko Murwantoko
Keyword(s):  
Next Generation Sequencing ◽  
Bacterial Community ◽  
16S Rrna ◽  
Community Composition ◽  
Water Samples ◽  
Bacterial Abundance ◽  
Rrna Gene ◽  
Next Generation ◽  
Aquaculture System ◽  
Generation Sequencing

Abstract. Wijayanti KAN, Istiqomah I, Murwantoko. 2021. Bacterial abundance and community composition in green, brown and red water from intensive Catfish (Clarias sp.) culture ponds in Yogyakarta, Indonesia. Biodiversitas 22: 3677-3684. Catfish (Clarias sp.) is an important aquaculture commodity in Indonesia and cultured in an intensive system. Microorganisms play an important role in maintaining water quality of aquaculture system. The objective of this study was to determine the bacterial abundance and community composition of green, brown and red water collected from intensive catfish culture ponds in Yogyakarta using next-generation sequencing method. The water samples were collected from intensive catfish culture ponds with different colors, namely green, brown and red ponds located in Yogyakarta. The DNA from water samples was extracted using DNA extraction kit and used as template for 16S rRNA amplification. The V3-V4 hypervariable regions of the 16S rRNA gene were amplified apply for next-generation sequencing technology. This study could explore effectively the bacterial community in water samples. The bacterial communities in this catfish culture water showed higher bacterial richness compared to the other aquaculture system. The diversity of the green, brown and red catfish culture water ponds was similar with the number OTUs of the green, brown and red water samples, which were 1269; 1387 and 1323 OTUs respectively. The 694 OTUs (34.42%) were common core microbiomes in all catfish culture ponds, the 212 OTUs (10.51%) are present on green and brown water ponds, the 182 OTUs (9.02%) were on green and red water ponds, and the 183 OTUs (9.07%) were present on green and brown water ponds. However, the composition of the bacterial community was different. The most dominant phylum in green and brown water ponds was Proteobacteria with relative abundance in green water and brown water 71.6% and 47.0% respectively, whereas, the most dominant phylum in red water was Firmicutes (29.5%). The dominance of Firmicutes phylum in red water ponds may be caused by application of probiotic bacteria, the high organic content, and low oxygen concentration.

scholarly journals CHLORINE DEMAND AND BACTERIAL ABUNDANCE OF SHRIMP POND WATER UNDER DIFFERENT SUSPENDED SOLID CONCENTRATIONS

2017 ◽  
Vol 7 (1) ◽  
pp. 16
Author(s):  
Husnah Husnah
Keyword(s):  
Bacterial Abundance ◽  
Pond Water ◽  
Suspended Solid ◽  
Indigenous Bacteria ◽  
Shrimp Pond ◽  
Chlorine Demand ◽  
Brackish Pond

An experiment has been conducted to evaluate the chlorine demand and efficacy of 6 chlorine concentrations in brackish pond water, to control indigenous bacteria in shrimps pond.

scholarly journals Bacterial abundance and diversity in pond water supplied with different feeds

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Ya Qin ◽  
Jie Hou ◽  
Ming Deng ◽  
Quansheng Liu ◽  
Chongwei Wu ◽  
...  
Keyword(s):  
Bacterial Abundance ◽  
Pond Water ◽  
Abundance And Diversity

Top-down and bottom-up induced shifts in bacterial abundance, production and community composition in an experimentally divided humic lake

2008 ◽  
Vol 10 (3) ◽  
pp. 635-652 ◽  
Author(s):  
Hans-Peter Grossart ◽  
Jan Jezbera ◽  
Karel Horňák ◽  
Kristine Michelle L. Hutalle ◽  
Ulrike Buck ◽  
...  
Keyword(s):  
Community Composition ◽  
Bacterial Abundance ◽  
Humic Lake ◽  
Top Down ◽  
Bottom Up

scholarly journals Bacterial Loads in Shrimp and Fish Hatchery Environments of Bangladesh

2017 ◽  
Vol 43 (1) ◽  
pp. 37-47
Author(s):  
Md Abdur Razzak Hasan ◽  
Md Inja Mamun Haque ◽  
Anwar Hossain ◽  
Mahmud Hasan ◽  
Mohammad Shamsur Rahman
Keyword(s):  
Water Sample ◽  
Water Samples ◽  
Bacterial Abundance ◽  
Bacterial Load ◽  
Age Groups ◽  
Bacterial Count ◽  
Pond Water ◽  
Bacterial Density ◽  
Live Food ◽  
Fish Hatchery

Artemia hatching tank of Cox’s Bazar hatchery had similar total bacterial build up (2.59 ± 0.10×107cfu/g) in the water sampled and in the shrimp post larvae (PL) sampled at stage 10 and 12 (2.37 ± 0.11×107cfu/g and 2.42 ± 0.10×107cfu/g, respectively). In MA plate, no significant differences was observed in the bacterial count of these samples. Similar result was observed for the total presumptive vibrio count in TCBS plates ranging from 3.8 ± 0.60×103cfu/g to1.62 ± 0.50×103cfu/g.Total bacterial load (7.5 ± 0.11×107) measured in the water sampled from 25 day old fry rearing pond of tilapia from Mymensingh Hatchery, was similar to that of 33 day old fry (8.6 ± .66×107). The bacterial density found in the 25 (1.6 ± 0.50×107), 28 (3.12 ± 0.14×107) and 40 day old fry (6.46 ± 1.52×106) samples was similar but significantly different from the sample of 33 day old fry and the water sample of the pond of 25 day old fry. In TCBS plate, bacterial abundance detected in the samples across all four age groups was similar (25 day old fry: 4.21 ± 3.79×103; 28 day old fry: 4.90 ± 3.50×103; 33 day old fry: 1.08 ± 0.12×103; 40 day old fry: 7.04 ± 2.08×103). In finfish hatchery of Bogra, the overall bacterial build up (2.03 ± 0.31×108) found in the samples of zeol fish fry in NA plate was significantly higher than that of the corresponding rearing pond water (2.11 ± 0.459×107) and the water of the live food rearing tank ( 8.43 ± 0.57×106). Similar to that, TCBS plates had 2.3-, and 5.09-folds higher bacterial load (1.08± 0.25×103) in the samples of fish fry than in the samples of the corresponding water samples and water samples of the live food rearing tank, respectively (4.70 ±w 1.67×102 and 2.12 ± 0.28×102). Asiat. Soc. Bangladesh, Sci. 43(1): 37-47, June 2017

scholarly journals Vegetation-Dependent Response to Drought in Salt Marsh Ammonia-Oxidizer Communities

2019 ◽  
Vol 8 (1) ◽  
pp. 9
Author(s):  
Jack K. Beltz ◽  
Hayley McMahon ◽  
Isis Torres Nunez ◽  
Anne E. Bernhard
Keyword(s):  
Salt Marsh ◽  
Community Composition ◽  
Bacterial Abundance ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Ammonia Oxidizer ◽  
N Availability ◽  
Ammonia Oxidizing Archaea ◽  
Vegetation Zones ◽  
Total Bacterial Community

We investigated the impacts of drought on ammonia-oxidizing archaea (AOA) and bacteria (AOB) in a salt marsh and compared the response to the total bacterial community. We analyzed abundance and community composition of amoA genes by QPCR and TRFLP, respectively, in three vegetation zones in 2014 (pre-drought), 2016 (drought), and 2017 (post-drought), and analyzed bacterial 16S rRNA genes by QPCR, TRFLP, and MiSeq analyses. AOA and AOB abundance in the Spartina patens zone increased significantly in 2016, while abundance decreased in the tall S. alterniflora zone, and showed little change in the short S. alterniflora zone. Total bacterial abundance declined annually in all vegetation zones. Significant shifts in community composition were detected in 2016 in two of the three vegetation zones for AOA and AOB, and in all three vegetation zones for total bacteria. Abundance and community composition of AOA and AOB returned to pre-drought conditions by 2017, while bacterial abundance continued to decline, suggesting that nitrifiers may be more resilient to drought than other bacterial communities. Finding vegetation-specific drought responses among N-cycling microbes may have broad implications for changes in N availability and marsh productivity, particularly if vegetation patterns continue to shift as predicted due to sea level rise.

scholarly journals Nitrogen Fertilizer Driven Bacterial Community Structure in a Semi-Arid Region of Northeast China

2021 ◽  
Vol 13 (21) ◽  
pp. 11967
Author(s):  
Meng Wang ◽  
Ling Wang ◽  
Qian Li ◽  
Hang Liu ◽  
Yuan Lin ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Community Composition ◽  
Bacterial Community Structure ◽  
Bacterial Abundance ◽  
Structure Stability ◽  
N Application ◽  
N Cycle ◽  
Application Rates ◽  
Semi Arid

The soil nitrogen (N) cycle is an essential role of the biogeochemical cycle. Bacteria play an irreplaceable part in the soil N cycle, but the impact of different N gradients on bacterial communities remains unclear. The purpose of this research was to explore the bacterial abundance, community composition, and diversity under different N application rates in a water-limited area. We investigated the bacterial abundance, diversity, community composition, and structure under five different N gradients (0, 90, 150, 210, and 270 kg ha−1) using real-time quantitative PCR and high-throughput sequencing, and then explored bacterial functional groups with FAPROTAX. N application significantly affected bacterial abundance and community composition. Bacterial diversity was enhanced at low N application rates and reduced at higher N application rates. Principal coordinate analysis showed that bacterial community structure was separated into two groups between low N application rates and high N application rates; these differences in bacterial community structure may be driven by available nitrogen (AN). The results of FAPROTAX revealed that N application promoted the functions of Aerobic_nitrite_oxidation, Nitrate_reduction, and Aerobic_ammonia_oxidation, but inhibited the Nitrogen_fixation function of the bacterial community. The high N network caused the reduction of network structure stability. Our results revealed that N fertilizer driven bacterial community structure and soil nutrients were the main influential factors in the variation of bacterial community structure. We suggest that the optimal N application rate in this study may be approximately 150 kg ha−1, based on the variations of soil properties and bacterial community structure in semi-arid areas.

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