scholarly journals NsrR1, a Nitrogen Stress-Repressed sRNA, Contributes to the Regulation of nblA in Nostoc sp. PCC 7120

2018 ◽  
Vol 9 ◽  
Author(s):  
Isidro Álvarez-Escribano ◽  
Agustín Vioque ◽  
Alicia M. Muro-Pastor
Keyword(s):  
Nitrogen Stress ◽  
Pcc 7120

scholarly journals NsiR3, a nitrogen stress‐inducible small RNA, regulates proline oxidase expression in the cyanobacterium Nostoc sp. PCC 7120

FEBS Journal ◽  
2020 ◽  
Author(s):  
Isidro Álvarez‐Escribano ◽  
Manuel Brenes‐Álvarez ◽  
Elvira Olmedo‐Verd ◽  
Jens Georg ◽  
Wolfgang R. Hess ◽  
...  
Keyword(s):  
Small Rna ◽  
Nitrogen Stress ◽  
Proline Oxidase ◽  
Pcc 7120

scholarly journals Transcriptional and translational analysis of ferredoxin and flavodoxin under iron and nitrogen stress in Anabaena sp. strain PCC 7120.

1994 ◽  
Vol 176 (23) ◽  
pp. 7409-7411 ◽  
Author(s):  
P Razquin ◽  
S Schmitz ◽  
M F Fillat ◽  
M L Peleato ◽  
H Böhme
Keyword(s):  
Nitrogen Stress ◽  
Anabaena Sp ◽  
Pcc 7120

scholarly journals The Nitrogen Stress-Repressed sRNA NsrR1 Regulates Expression of all1871, a Gene Required for Diazotrophic Growth in Nostoc sp. PCC 7120

Life ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 54 ◽  
Author(s):  
Isidro Álvarez-Escribano ◽  
Manuel Brenes-Álvarez ◽  
Elvira Olmedo-Verd ◽  
Agustín Vioque ◽  
Alicia M. Muro-Pastor
Keyword(s):  
Nitrogen Availability ◽  
Specific Interaction ◽  
Nitrogen Stress ◽  
Transcriptional Level ◽  
Gene Encoding ◽  
Bacterial Gene ◽  
Mrna Targets ◽  
Bacterial Gene Expression ◽  
Small Regulatory Rnas ◽  
Pcc 7120

Small regulatory RNAs (sRNAs) are post-transcriptional regulators of bacterial gene expression. In cyanobacteria, the responses to nitrogen availability, that are mostly controlled at the transcriptional level by NtcA, involve also at least two small RNAs, namely NsiR4 (nitrogen stress-induced RNA 4) and NsrR1 (nitrogen stress-repressed RNA 1). Prediction of possible mRNA targets regulated by NsrR1 in Nostoc sp. PCC 7120 allowed, in addition to previously described nblA, the identification of all1871, a nitrogen-regulated gene encoding a protein of unknown function that we describe here as required for growth at the expense of atmospheric nitrogen (N2). We show that transcription of all1871 is induced upon nitrogen step-down independently of NtcA. All1871 accumulation is repressed by NsrR1 and its expression is stronger in heterocysts, specialized cells devoted to N2 fixation. We demonstrate specific interaction between NsrR1 and the 5′ untranslated region (UTR) of the all1871 mRNA, that leads to decreased expression of all1871. Because transcription of NsrR1 is partially repressed by NtcA, post-transcriptional regulation by NsrR1 would constitute an indirect way of NtcA-mediated regulation of all1871.

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

Target Gene Inactivation in Cyanobacterium Anabaena sp. PCC 7120

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (15) ◽  
Author(s):  
Kangming Chen ◽  
Huilan Zhu ◽  
Liping Gu ◽  
Shengni Tian ◽  
Ruanbao Zhou
Keyword(s):  
Target Gene ◽  
Gene Inactivation ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

scholarly journals Gluconacetobacter diazotrophicus Pal5 Enhances Plant Robustness Status under the Combination of Moderate Drought and Low Nitrogen Stress in Zea mays L.

2021 ◽  
Vol 9 (4) ◽  
pp. 870
Author(s):  
Muhammad Aammar Tufail ◽  
María Touceda-González ◽  
Ilaria Pertot ◽  
Ralf-Udo Ehlers
Keyword(s):  
Zea Mays ◽  
Plant Growth ◽  
Zea Mays L ◽  
Growth Promotion ◽  
Nitrogen Stress ◽  
Rrna Gene ◽  
N Fixation ◽  
Gluconacetobacter Diazotrophicus ◽  
Maize Plants ◽  
Low Nitrogen

Plant growth promoting endophytic bacteria, which can fix nitrogen, plays a vital role in plant growth promotion. Previous authors have evaluated the effect of Gluconacetobacter diazotrophicus Pal5 inoculation on plants subjected to different sources of abiotic stress on an individual basis. The present study aimed to appraise the effect of G. diazotrophicus inoculation on the amelioration of the individual and combined effects of drought and nitrogen stress in maize plants (Zea mays L.). A pot experiment was conducted whereby treatments consisted of maize plants cultivated under drought stress, in soil with a low nitrogen concentration and these two stress sources combined, with and without G. diazotrophicus seed inoculation. The inoculated plants showed increased plant biomass, chlorophyll content, plant nitrogen uptake, and water use efficiency. A general increase in copy numbers of G. diazotrophicus, based on 16S rRNA gene quantification, was detected under combined moderate stress, in addition to an increase in the abundance of genes involved in N fixation (nifH). Endophytic colonization of bacteria was negatively affected by severe stress treatments. Overall, G. diazotrophicus Pal5 can be considered as an effective tool to increase maize crop production under drought conditions with low application of nitrogen fertilizer.

scholarly journals Combining Ability and Heterosis of Algerian Saharan Maize Populations (Zea mays L.) for Tolerance to No-Nitrogen Fertilization and Drought

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 492
Author(s):  
Meriem Riache ◽  
Pedro Revilla ◽  
Oula Maafi ◽  
Rosa Ana Malvar ◽  
Abderahmane Djemel
Keyword(s):  
Zea Mays ◽  
Stress Tolerance ◽  
Nitrogen Fertilization ◽  
Recurrent Selection ◽  
Zea Mays L ◽  
Base Material ◽  
N Fertilization ◽  
Nitrogen Stress ◽  
Reciprocal Recurrent Selection ◽  
Maize Populations

Drought and low nitrogen are major stresses for maize (Zea mays L.), and maize populations from the Sahara Desert are potential sources of stress tolerance. The objectives were to assess the tolerance and varietal and heterosis effects of Algerian populations under no-nitrogen fertilization and water stress. A diallel among six Algerian maize population was evaluated under drought (300 mm irrigation) vs. control (600 mm) and no-nitrogen fertilization vs. 120 kh ha−1 N fertilization. Genotypes showed significant differences and genetic effects for water- and nitrogen-stress tolerance. We propose a reciprocal recurrent selection to take advantage of additive and non-additive effects, using AOR and IGS, since they showed good performance in optimum and stress conditions, for improving yield heterosis for AOR × IGS. Negative effects are not expected on plant height, anthesis–silking interval or early vigor. These populations and BAH could be sources of inbred lines tolerant to drought and no-nitrogen fertilization. There was no relationship between origin and genetic group and stress tolerance per se or as parents of tolerant crosses. These populations and crosses could be used as base material among Algerian populations, for breeding programs focusing on tolerance to water or nitrogen stress.

scholarly journals The cyanobacteriumAnabaenasp. PCC 7120 has two distinct β-carotene ketolases: CrtO for echinenone and CrtW for ketomyxol synthesis

FEBS Letters ◽  
2005 ◽  
Vol 579 (27) ◽  
pp. 6111-6114 ◽  
Author(s):  
Mari Mochimaru ◽  
Hajime Masukawa ◽  
Shinichi Takaichi
Keyword(s):  
Pcc 7120 ◽  
Β Carotene
Sign in / Sign up

Export Citation Format

Share Document