scholarly journals Establishment and Evaluation of a Stable Bovine Thyroid Cell Line for Investigating Foot-and-Mouth Disease Virus

2018 ◽  
Vol 9 ◽  
Author(s):  
Ruoqing Mao ◽  
Dehui Sun ◽  
Fan Yang ◽  
Hong Tian ◽  
Zixiang Zhu ◽  
...  
Keyword(s):  
Cell Line ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Thyroid Cell ◽  
Thyroid Cell Line ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Bovine Thyroid

scholarly journals An adventitious agent-free clonal cell line that is highly susceptible to foot -and-mouth disease virus

Biologicals ◽  
2021 ◽  
Author(s):  
M. LaRocco ◽  
Z. Ahmed ◽  
M. Rodriguez-Calzada ◽  
P.A. Azzinaro ◽  
R. Barrette ◽  
...  
Keyword(s):  
Cell Line ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Clonal Cell Line ◽  
Clonal Cell ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Targeted Modification of the Foot-And-Mouth Disease Virus Genome for Quick Cell Culture Adaptation

2020 ◽  
Author(s):  
Veronika Dill ◽  
Aline Zimmer ◽  
Martin Beer ◽  
Michael Eschbaumer
Keyword(s):  
Cell Line ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Productive Infection ◽  
Single Amino Acid ◽  
Adaptive Mutations ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Foot-and-mouth disease virus (FMDV) causes the highly contagious foot-and-mouth disease, which is characterized by the appearance of vesicles in and around the mouth and feet of cloven-hoofed animals. BHK21 cells are the cell line of choice for the propagation of FMDV for vaccine production world-wide but vary in their susceptibility for different FMDV strains. Previous studies showed that the FMDV resistance of a certain BHK cell line can be overcome by using a closely related but permissive cell line for the pre-adaptation of the virus, but the adapted strains were found to harbor several capsid mutations. In this study, these adaptive mutations were introduced into the original Asia-1 Shamir isolate individually or in combination to create a panel of 17 Asia-1 mutants by reverse genetics and examine the effects of the mutations on receptor usage, viral growth, immunogenicity and stability. A single amino acid exchange from glutamic acid to lysine at position 202 in VP1 turned out to be of major importance for productive infection of the suspension cell line BHK-2P. In consequence, two traditionally passage-derived strains and two recombinant viruses with a minimum set of mutations were tested in vivo. While the passaged-derived viruses showed a reduced particle stability, the genetically modified viruses were more stable but did not confer a protective immune response against the original virus isolate.

scholarly journals Targeted Modification of the Foot-And-Mouth Disease Virus Genome for Quick Cell Culture Adaptation

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 583
Author(s):  
Veronika Dill ◽  
Aline Zimmer ◽  
Martin Beer ◽  
Michael Eschbaumer
Keyword(s):  
Cell Line ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Productive Infection ◽  
Single Amino Acid ◽  
Adaptive Mutations ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Foot-and-mouth disease virus (FMDV) causes the highly contagious foot-and-mouth disease, which is characterized by the appearance of vesicles in and around the mouth and feet of cloven-hoofed animals. BHK-21 cells are the cell line of choice for the propagation of FMDV for vaccine production worldwide but vary in their susceptibility for different FMDV strains. Previous studies showed that the FMDV resistance of a certain BHK cell line can be overcome by using a closely related but permissive cell line for the pre-adaptation of the virus, but the adapted strains were found to harbor several capsid mutations. In this study, these adaptive mutations were introduced into the original Asia-1 Shamir isolate individually or in combination to create a panel of 17 Asia-1 mutants by reverse genetics and examine the effects of the mutations on receptor usage, viral growth, immunogenicity and stability. A single amino acid exchange from glutamic acid to lysine at position 202 in VP1 turned out to be of major importance for productive infection of the suspension cell line BHK-2P. In consequence, two traditionally passage-derived strains and two recombinant viruses with a minimum set of mutations were tested in vivo. While the passaged-derived viruses showed a reduced particle stability, the genetically modified viruses were more stable but did not confer a protective immune response against the original virus isolate.

Selection of a stable clone of the MVPK-1 fetal porcine kidney cell for assays of foot-and-mouth disease virus

1977 ◽  
Vol 23 (3) ◽  
pp. 295-299 ◽  
Author(s):  
Stephen K. Dinka ◽  
Lois M. Swaney ◽  
John W. McVicar
Keyword(s):  
Cell Line ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Kidney Cells ◽  
Porcine Kidney ◽  
Bovine Kidney ◽  
Fmd Virus ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Bovine Thyroid

The MVPK-1 cell line, derived from fetal porcine kidney cells, supports the replication of foot-and-mouth disease (FMD) virus. The cell line was adapted to grow in medium containing 5% bovine serum. The susceptibility of the adapted cells decreased as they aged at 37 °C. Various clones were isolated from the adapted cells and their growth characteristics and sustained susceptibility to FMD virus were compared. Clone 7 maintained uniform susceptibility to FMD virus over a 3-day period at 37 °C and proved superior to other clones in the characteristics studied. The clone has maintained satisfactory susceptibility to FMD virus through 40 subcultures. Clone 7 can replace primary bovine kidney cells for routine viral assays, but cannot detect as much FMD virus in animal specimens as primary bovine kidney, bovine thyroid, or swine kidney cells.

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