Growth of Foot-and-Mouth Disease Virus in a Fibroblastic Cell Line Derived from Hamster Kidneys

Nature ◽  
1962 ◽  
Vol 194 (4825) ◽  
pp. 253-255 ◽  
Author(s):  
G. N. MOWAT ◽  
W. G. CHAPMAN
Keyword(s):  
Cell Line ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Fibroblastic Cell ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals An adventitious agent-free clonal cell line that is highly susceptible to foot -and-mouth disease virus

Biologicals ◽  
2021 ◽  
Author(s):  
M. LaRocco ◽  
Z. Ahmed ◽  
M. Rodriguez-Calzada ◽  
P.A. Azzinaro ◽  
R. Barrette ◽  
...  
Keyword(s):  
Cell Line ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Clonal Cell Line ◽  
Clonal Cell ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Targeted Modification of the Foot-And-Mouth Disease Virus Genome for Quick Cell Culture Adaptation

2020 ◽  
Author(s):  
Veronika Dill ◽  
Aline Zimmer ◽  
Martin Beer ◽  
Michael Eschbaumer
Keyword(s):  
Cell Line ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Productive Infection ◽  
Single Amino Acid ◽  
Adaptive Mutations ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Foot-and-mouth disease virus (FMDV) causes the highly contagious foot-and-mouth disease, which is characterized by the appearance of vesicles in and around the mouth and feet of cloven-hoofed animals. BHK21 cells are the cell line of choice for the propagation of FMDV for vaccine production world-wide but vary in their susceptibility for different FMDV strains. Previous studies showed that the FMDV resistance of a certain BHK cell line can be overcome by using a closely related but permissive cell line for the pre-adaptation of the virus, but the adapted strains were found to harbor several capsid mutations. In this study, these adaptive mutations were introduced into the original Asia-1 Shamir isolate individually or in combination to create a panel of 17 Asia-1 mutants by reverse genetics and examine the effects of the mutations on receptor usage, viral growth, immunogenicity and stability. A single amino acid exchange from glutamic acid to lysine at position 202 in VP1 turned out to be of major importance for productive infection of the suspension cell line BHK-2P. In consequence, two traditionally passage-derived strains and two recombinant viruses with a minimum set of mutations were tested in vivo. While the passaged-derived viruses showed a reduced particle stability, the genetically modified viruses were more stable but did not confer a protective immune response against the original virus isolate.

scholarly journals Targeted Modification of the Foot-And-Mouth Disease Virus Genome for Quick Cell Culture Adaptation

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 583
Author(s):  
Veronika Dill ◽  
Aline Zimmer ◽  
Martin Beer ◽  
Michael Eschbaumer
Keyword(s):  
Cell Line ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Productive Infection ◽  
Single Amino Acid ◽  
Adaptive Mutations ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Foot-and-mouth disease virus (FMDV) causes the highly contagious foot-and-mouth disease, which is characterized by the appearance of vesicles in and around the mouth and feet of cloven-hoofed animals. BHK-21 cells are the cell line of choice for the propagation of FMDV for vaccine production worldwide but vary in their susceptibility for different FMDV strains. Previous studies showed that the FMDV resistance of a certain BHK cell line can be overcome by using a closely related but permissive cell line for the pre-adaptation of the virus, but the adapted strains were found to harbor several capsid mutations. In this study, these adaptive mutations were introduced into the original Asia-1 Shamir isolate individually or in combination to create a panel of 17 Asia-1 mutants by reverse genetics and examine the effects of the mutations on receptor usage, viral growth, immunogenicity and stability. A single amino acid exchange from glutamic acid to lysine at position 202 in VP1 turned out to be of major importance for productive infection of the suspension cell line BHK-2P. In consequence, two traditionally passage-derived strains and two recombinant viruses with a minimum set of mutations were tested in vivo. While the passaged-derived viruses showed a reduced particle stability, the genetically modified viruses were more stable but did not confer a protective immune response against the original virus isolate.

scholarly journals Adaptation of Three Different BLRI Strain (O, A, Asia 1) of Foot and Mouth Disease Virus Serotypes in Baby Hamster Kidney-21 Cell Line

2017 ◽  
Vol 5 (2) ◽  
pp. 11-15
Author(s):  
Mohammad Showkat Mahmud ◽  
Eusha Islam ◽  
Mohammed Abdus Samad ◽  
Md. Rezaul Karim ◽  
Amal Kumar Saha ◽  
...  
Keyword(s):  
Cell Line ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Baby Hamster Kidney ◽  
Mouth Disease Virus ◽  
Foot And Mouth
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