scholarly journals A Broad Spectrum Racemase in Pseudomonas putida KT2440 Plays a Key Role in Amino Acid Catabolism

2018 ◽  
Vol 9 ◽  
Author(s):  
Atanas D. Radkov ◽  
Luke A. Moe
Keyword(s):  
Amino Acid ◽  
Pseudomonas Putida ◽  
Broad Spectrum ◽  
Amino Acid Catabolism ◽  
Pseudomonas Putida Kt2440

scholarly journals Nitrogen metabolism in Pseudomonas putida: functional analysis using random barcode transposon sequencing

2021 ◽  
Author(s):  
Matthias Schmidt ◽  
Allison N. Pearson ◽  
Matthew R. Incha ◽  
Mitchell G. Thompson ◽  
Edward E. K. Baidoo ◽  
...  
Keyword(s):  
Amino Acid ◽  
Pseudomonas Putida ◽  
Transcriptional Regulators ◽  
Drop Out ◽  
Visualization Tool ◽  
Acid Biosynthesis ◽  
Pseudomonas Putida Kt2440 ◽  
Related Proteins ◽  
Nitrogen Containing Compounds

Pseudomonas putida KT2440 has long been studied for its diverse and robust metabolisms, yet many genes and proteins imparting these growth capacities remain uncharacterized. Using pooled mutant fitness assays, we identified genes and proteins involved in the assimilation of 52 different nitrogen containing compounds. To assay amino acid biosynthesis, 19 amino acid drop-out conditions were also tested. From these 71 conditions, significant fitness phenotypes were elicited in 672 different genes including 100 transcriptional regulators and 112 transport-related proteins. We divide these conditions into 6 classes, and propose assimilatory pathways for the compounds based on this wealth of genetic data. To complement these data, we characterize the substrate range of three promiscuous aminotransferases relevant to metabolic engineering efforts in vitro. Furthermore, we examine the specificity of five transcriptional regulators, explaining some fitness data results and exploring their potential to be developed into useful synthetic biology tools. In addition, we use manifold learning to create an interactive visualization tool for interpreting our BarSeq data, which will improve the accessibility and utility of this work to other researchers.

scholarly journals Common Enzymes of Branched-Chain Amino Acid Catabolism in Pseudomonas putida

1973 ◽  
Vol 115 (1) ◽  
pp. 198-204 ◽  
Author(s):  
Ronald R. Martin ◽  
Vincent D. Marshall ◽  
John R. Sokatch ◽  
Leon Unger
Keyword(s):  
Amino Acid ◽  
Pseudomonas Putida ◽  
Amino Acid Catabolism ◽  
Branched Chain Amino Acid ◽  
Branched Chain

scholarly journals AMINO ACID CATABOLISM

1938 ◽  
Vol 122 (2) ◽  
pp. 491-496 ◽  
Author(s):  
Fred H. Snyder ◽  
Ralph C. Corley
Keyword(s):  
Amino Acid ◽  
Amino Acid Catabolism

Nitrogen regulation of amino acid catabolism in Neurospora crassa

1978 ◽  
Vol 16 (3-4) ◽  
pp. 343-354 ◽  
Author(s):  
Thomas J. Facklam ◽  
George A. Marzluf
Keyword(s):  
Amino Acid ◽  
Neurospora Crassa ◽  
Nitrogen Regulation ◽  
Amino Acid Catabolism
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