scholarly journals The Presence or Absence of Intestinal Microbiota Affects Lipid Deposition and Related Genes Expression in Zebrafish (Danio rerio)

2018 ◽  
Vol 9 ◽  
Author(s):  
Yi Sheng ◽  
Hui Ren ◽  
Samwel M. Limbu ◽  
Yuhong Sun ◽  
Fang Qiao ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Danio Rerio ◽  
Lipid Deposition ◽  
Genes Expression

Identification and isolation of a BTB–POZ-containing gene expressed in oocytes and early embryos of the zebrafish Danio rerio

Genome ◽  
2006 ◽  
Vol 49 (7) ◽  
pp. 808-814
Author(s):  
T H.L Smith ◽  
K Stedronsky ◽  
B Morgan ◽  
R A McGowan
Keyword(s):  
Expression Pattern ◽  
Danio Rerio ◽  
Early Cleavage ◽  
Genes Expression ◽  
Early Embryos

In this report, we describe the cloning of a cDNA from the zebrafish Danio rerio encoding a protein containing a BTB–POZ domain closely resembling the BTBD1 and BTBD2 proteins previously identified in mammals. However, unlike other BTB–POZ-containing genes, expression of this gene in adults is most abundant in oocytes, where the RNA can be detected at all stages of oogenesis examined. The presence of the RNA persists through early cleavage, but is decreased significantly by gastrulation. Although the function of this gene has yet to be determined, its resemblance to the BTB–POZ family of genes coupled with its expression pattern suggests that it may have an important function in oogenesis and (or) early zebrafish development.Key words: BTBD1, BTBD2, BTB–POZ domain, kelch superfamily, PHR domain.

Concentration-dependent effects of 17β-estradiol and bisphenol A on lipid deposition, inflammation and antioxidant response in male zebrafish (Danio rerio)

Chemosphere ◽  
2019 ◽  
Vol 237 ◽  
pp. 124422 ◽  
Author(s):  
Sheng-Xiang Sun ◽  
Yun-Ni Zhang ◽  
Dong-Liang Lu ◽  
Wei-Li Wang ◽  
Samwel Mchele Limbu ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Danio Rerio ◽  
Antioxidant Response ◽  
Lipid Deposition ◽  
17Β Estradiol

scholarly journals Study of GH and Ghrelin genes expression during the larvae developmental period in Danio rerio

2019 ◽  
Vol 6 (2) ◽  
pp. 148-154
Author(s):  
Hamed Paknejad ◽  
Tayebeh Enayat Gholampour ◽  
Roghayeh Safari ◽  
Seyed Hossein Hossenifar ◽  
◽  
...  
Keyword(s):  
Danio Rerio ◽  
Developmental Period ◽  
Genes Expression

scholarly journals The Association between Gut Microbiota and Intestinal Toll-like Receptors Genes Expression in Adenomatous and Colorectal Cancerous patients

2020 ◽  
Author(s):  
Sama Rezasoltani ◽  
Maryam Sharafkhah ◽  
Hamid Asadzadeh Aghdaei ◽  
Meysam Olfatifar ◽  
Ehsan Nazemalhosseini Mojarad ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Gut Microbiota ◽  
Intestinal Microbiota ◽  
Intestinal Inflammation ◽  
Inflammatory Responses ◽  
Bacteroides Fragilis ◽  
Normal Group ◽  
Adenomatous Polyposis ◽  
Toll Like Receptors ◽  
Genes Expression

Abstract Background: Toll-like receptor (TLR) signaling has been implicated in colorectal cancer (CRC) development. Intestinal microbiota can affect the expression of TLRs, which may induce inflammatory responses and impair the gut homeostasis. Here, we aimed to evaluate certain intestinal microbiota related to TLRs expression in colonic tissues of adenomatous polyposis and CRC patients. Results: Fecal and colonic tissue samples were collected from normal controls (NC), adenomatous (AP) cases and (CRC) patients via colonoscopy for CRC screening during 2016 to 2018. Fecal samples were collected to analyze intestinal bacteria including Streptococcus bovis , Enterococcus faecalis , Bacteroides fragilis , enterotoxigenic Bacteroides fragilis (ETBF) , Fusobacterium nucleatum , Porphyromonas gingivalis, Porphyromonas spp . and Roseburia spp . by real-time PCR. Gene expression of TLR2, TLR4 and TLR5 was examined in colonic tissues by qRT-PCR. Different abundant of gut bacteria were achieved in NC, AP and CRC groups. The genes expression of TLR2, TLR4 and TLR5 were significantly different in AP and CRC cases vs. normal group (P value <0.05). There was a significant relationship between TLR2, TLR4, TLR5 genes expression and Roseburia spp., P. gingivalis and ETBF quantity in normal group. Also significant association between TLR2, TLR4 genes expression levels and the quantity of S.bovis , ETBF, Roseburia spp. and E. faecalis in AP and CRC cases were achieved. Conclusion : Intestinal expression of TLR2, TLR4 and TLR5 is dynamic and depends on gut microbiota. Hence, altered immune activation in response to dysbiotic microbiota may promote intestinal inflammation in a group of patients with AP and CRC. Keyword: Adenomatous polyposis; colorectal cancer; gut microbiota; Toll-like receptors; intestinal inflammation

scholarly journals GSK-3β at the Crossroads in Regulating Protein Synthesis and Lipid Deposition in Zebrafish

Cells ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 205 ◽  
Author(s):  
Yaqi Gu ◽  
Lili Gao ◽  
Qiang Han ◽  
Ao Li ◽  
Hairui Yu ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Protein Expression ◽  
Danio Rerio ◽  
Fatty Acid Synthase ◽  
Binding Protein ◽  
Lipid Deposition ◽  
Nonesterified Fatty Acids ◽  
Mrna And Protein Expression ◽  
Ribosomal S6 Kinase ◽  
Gsk 3Β

In this study, the mechanism by which GSK-3β regulates protein synthesis and lipid deposition was investigated in zebrafish (Danio rerio). The vector of pEGFP-N1-GSK-3β was constructed and injected into the muscle of zebrafish. It was found that the mRNA and protein expression of tuberous sclerosis complex 2 (TSC2) was significantly increased. However, the mRNA and protein expression of mammalian target of rapamycin (mTOR), p70 ribosomal S6 kinase 1 (S6K1), and 4E-binding protein 1 (4EBP1) was significantly decreased by the pEGFP-N1-GSK-3β vector in the muscle of zebrafish. In addition, the mRNA and protein expression of β-catenin, CCAAT/enhancer binding protein α (C/EBPα), and peroxisome proliferators-activated receptor γ (PPARγ) was significantly decreased, but the mRNA expression of fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), ATP-citrate lyase (ACL), and HMG-CoA reductase (HMGCR) was significantly increased by the pEGFP-N1-GSK-3β vector. The activity of FAS, ACC, ACL, and HMGCR as well as the content of triglyceride (TG), total cholesterol (TC), and nonesterified fatty acids (NEFA) were significantly increased by the pEGFP-N1-GSK-3β vector in the muscle of zebrafish. The content of free amino acids Arg, Lys, His, Phe, Leu, Ile, Val, and Thr was significantly decreased by the pEGFP-N1-GSK-3β vector. The results indicate that GSK-3β may participate in regulating protein synthesis via TSC2/mTOR signaling and regulating lipid deposition via β-catenin in the muscle of zebrafish (Danio rerio).

scholarly journals FoxO1 is a critical regulator of hepatocyte lipid deposition in chronic stress mice

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7668
Author(s):  
Yun-zi Liu ◽  
Wei Peng ◽  
Ji-kuai Chen ◽  
Wen-jun Su ◽  
Wen-jie Yan ◽  
...  
Keyword(s):  
Chronic Stress ◽  
Liver Lipid ◽  
Lipid Synthesis ◽  
Cellular Level ◽  
Lipid Deposition ◽  
Lipid Levels ◽  
Nonalcoholic Fatty Liver ◽  
Forkhead Box ◽  
Genes Expression ◽  
Biochemical Detection

Forkhead box O1 (FoxO1) is involved in lipid metabolisms. However, its role in chronic stress-related nonalcoholic fatty liver disease (NAFLD) is unclear. The scientific premise of our study was based on the finding that FoxO1 expression is increased in the liver of mice after chronic stress. It is important to understand the mechanisms involved in the activation of FoxO1 and how its function affects the liver lipid deposition. We employed a murine chronic stress model, in which mice were treated by plantar electrical stimulation and restraint for 6 weeks, and a cellular model, in which Hepa1–6 cells were treated with corticosterone. We also used a pharmacologic approach as1842856, a highly specific FoxO1 inhibitor. Lipid metabolism related genes levels were measured by qRT-PCR and the lipid levels by biochemical detection. We show that the level of FoxO1 is significantly elevated in the liver of chronic stress mice. Transcription factor FoxO1 regulates a lipid synthesis phenotype of hepatocyte that is involved in the development and progression of NAFLD. We have shown that inhibition of FoxO1 induced phenotypic conversion of hepatocytes and down-regulates lipid synthesis genes expression by hepatocytes, which contribute to lipid deposition in NAFLD. At the cellular level, the inhibitor of FoxO1 as1842856 can also attenuate the lipid deposition of Hepa1–6 cells induced by corticosterone. Targeting FoxO1 is a novel therapeutic target for chronic stress-related NAFLD.

Sign in / Sign up

Export Citation Format

Share Document