scholarly journals Study of GH and Ghrelin genes expression during the larvae developmental period in Danio rerio

2019 ◽  
Vol 6 (2) ◽  
pp. 148-154
Author(s):  
Hamed Paknejad ◽  
Tayebeh Enayat Gholampour ◽  
Roghayeh Safari ◽  
Seyed Hossein Hossenifar ◽  
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...  
Genome ◽  
2006 ◽  
Vol 49 (7) ◽  
pp. 808-814
Author(s):  
T H.L Smith ◽  
K Stedronsky ◽  
B Morgan ◽  
R A McGowan

In this report, we describe the cloning of a cDNA from the zebrafish Danio rerio encoding a protein containing a BTB–POZ domain closely resembling the BTBD1 and BTBD2 proteins previously identified in mammals. However, unlike other BTB–POZ-containing genes, expression of this gene in adults is most abundant in oocytes, where the RNA can be detected at all stages of oogenesis examined. The presence of the RNA persists through early cleavage, but is decreased significantly by gastrulation. Although the function of this gene has yet to be determined, its resemblance to the BTB–POZ family of genes coupled with its expression pattern suggests that it may have an important function in oogenesis and (or) early zebrafish development.Key words: BTBD1, BTBD2, BTB–POZ domain, kelch superfamily, PHR domain.


2017 ◽  
Author(s):  
Lianqiang Cheng ◽  
Xiaowen Wang ◽  
Shijun Li ◽  
Yongjun Liu ◽  
Zhengxin Guo

AbstractThe estrogen receptor (ER) genes, which encode a group of important ligand-activated transcriptional factors, can modulate estrogen-target gene activities. Zebrafish (Danio rerio) have three ER receptor genes, esr1, esr2a, and esr2b. In this study, we examined the mRNA expression levels of these ER receptors after treatment with mestranol (EE3ME). Zebrafish larvae were exposed to 0.01, 0.1, 1, and 10 mg/L from 6 hours post-fertilization (hpf) and the mRNA expression levels of the ER genes were determined at 24, 48, 72 and 96 hpf. Treatment with mestranol led to a significant stimulation of esr1 mRNA expression at lower concentration and reached maximum at 72 hpf, however, the esr1 mRNA levels were reduced at higher mestranol concentration during exposure. The gene expression of esr2b was markedly decreased and the esr2a remained unaffected at all concentration in the duration. Altogether, these results suggested mestranol might cause the disruption of endocrine activities in fish by mediating ER genes expression.


Author(s):  
Carmen García-Alba

This study is part of a larger research study (doctoral dissertation), in which a comparative study with adolescent samples is done: 50 anorexic restricting patients (ANP), 50 patients diagnosed with depression (DP) and 50 non patients (NP). The proposed objective is two-fold: 1) To try to clarify the existing relationship between Anorexia (AN) and Depression (D), investigated from diverse disciplines but without conclusive results. 2) To detect in the ANP personality different traits from those of other groups, which should, if possible, allow to detect them at an early stage for an adequate prognosis. The current article presents the Rorschach findings in relation to the cognitive functioning of the ANP. In them, the following has been detected: (1) An information processing similar to that of the other groups, even with a more complete (L ≤ .99), more complex (DQ+↑) and better discriminated (Zd↑) grasp of the stimulus; (2) Mediating processes very similar to those of the other groups, sharing with them the perceptive maladjustments (X–%↑) and an excessive individualism (Xu%↑); (3) A clearly differentiating ideation disorder. Definitely, the ANP use predominantly ideation (M↑), but their thought, usually well-adjusted (MQo↑), presents eventual operations of delusional type (MQnone↑). Above that, their thinking is marked by a great passivity (Mp↑), which makes them more vulnerable to accept ideas without criticizing them and it results in a very inefficient thinking, which spins around these concepts without finding solutions, entering into a sort of ruminating which is completely unproductive. The differences toward the obsessive pathology are established. The discriminant analysis conducted with all the Rorschach variables that resulted as significant throughout the research, provides quite a consistent function which discriminates the ANP: MQnone↑, Mp↑, FD↓, Ma↑, MQo↑, AdjD↑, Sum H↑, (H)↑. Based on this we can understand that these adolescents, being in a developmental period of big changes and disorientations in relation with their own image, confronted with life events, and possibly starting off with some biologic vulnerability: (1) Due to the alterations of their ideation, accept without criticism (Mp) irrational ideas dominating in our culture, in which slimness appears as the only model, synthesis of intelligence, beauty and success; remaining captured in this type of mental activity (MQnone), which they cannot escape nor criticize (Mp), despite they reason adequately on other topics (MQo); (2) Their alterations of self-perception [(H)] make them hide themselves in a fantasized image, which is the axis of their interests and the only thing that really matters to them; (3) The resources they have to decide on behaviors and to finish these deliberately (AdjD), and their scarce tendency to the introspection (FD) lead to their decision of not eating, based on distorted and passively accepted thinking, which has great power and thus, so difficult to modify. Finally, based on the Rorschach data obtained, the hypothesis of a personality disorder as underlying pathology is pointed out.


2019 ◽  
Vol 133 (2) ◽  
pp. 143-155 ◽  
Author(s):  
Vicenç Quera ◽  
Elisabet Gimeno ◽  
Francesc S. Beltran ◽  
Ruth Dolado

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