scholarly journals Diversity of Bacillus thuringiensis Strains From Qatar as Shown by Crystal Morphology, δ-Endotoxins and Cry Gene Content

2018 ◽  
Vol 9 ◽  
Author(s):  
Kavita Nair ◽  
Roda Al-Thani ◽  
Dhabia Al-Thani ◽  
Fatima Al-Yafei ◽  
Talaat Ahmed ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Crystal Morphology ◽  
Gene Content ◽  
Cry Gene

Insecticidal Activity of a Cry1Ca toxin of Bacillus thuringiensis Berliner (Firmicutes: Bacillaceae) and Its Synergism with the Cyt1Aa Toxin Against Aedes aegypti (Diptera: Culicidae)

2020 ◽  
Vol 57 (6) ◽  
pp. 1852-1856
Author(s):  
Sebastian E González-Villarreal ◽  
Mónica García-Montelongo ◽  
Jorge E Ibarra
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Synergistic Effect ◽  
Joint Action ◽  
Recombinant Strain ◽  
Crystal Morphology ◽  
Graphical Method ◽  
Toxic Activity ◽  
Lepidopteran Species ◽  
Gene Coding

Abstract The Cry1C protein family of Bacillus thuringiensis form bipyramidal crystals, which are commonly associated with toxic activity against lepidopteran species; however, some members of this family may also be toxic to dipterans. In the present work, the Cry1Ca16 protein, synthesized by the B. thuringiensis LBIT-1217 strain, was analyzed. The gene coding for this protein was amplified, sequenced, and cloned into the pSTAB vector, which was electro-transferred into the acrystalliferous B. thuringiensis 4Q7 strain. The recombinant strain showed the expected bipyramidal crystal morphology, identical to the original LBIT-1217 strain and exhibited toxicity against larvae of Aedes aegypti (Diptera). Pure crystals from the recombinant strain were used in bioassays against Ae. aegypti larvae, estimating an LC50 of 4.61 μg/ml. Further studies on Cry1Ca16 mosquitocidal potential included joint-action tests with the Cyt1Aa protein crystals from B. thuringiensis israelensis. An LC50 using pure Cyt1Aa crystals was estimated at 0.73 μg/ml, whereas an LC50 of 0.61 μg/ml was estimated when both toxins were tested together. Data from these bioassays was analyzed using joint-action tests such as the Tammes-Bakuniak graphical method and the formula proposed by Tabashnik (1992). Both tests clearly showed a synergistic effect between these two toxins.

Analysis of cry Gene Profiles in Bacillus Thuringiensis Strains Isolated During Epizootics in Cydia pomonella L.

2007 ◽  
Vol 55 (3) ◽  
pp. 217-222 ◽  
Author(s):  
Edyta Konecka ◽  
Adam Kaznowski ◽  
Jadwiga Ziemnicka ◽  
Kazimierz Ziemnicki ◽  
Halina Paetz
Keyword(s):  
Bacillus Thuringiensis ◽  
Cydia Pomonella ◽  
Cry Gene ◽  
Gene Profiles

Characterization of new Bacillus thuringiensis strains from Iran, based on cytocidal and insecticidal activity, proteomic analysis and gene content

BioControl ◽  
2018 ◽  
Vol 63 (6) ◽  
pp. 807-818 ◽  
Author(s):  
Ayda Khorramnejad ◽  
Reza Talaei-Hassanloui ◽  
Vahid Hosseininaveh ◽  
Yolanda Bel ◽  
Baltasar Escriche
Keyword(s):  
Bacillus Thuringiensis ◽  
Insecticidal Activity ◽  
Proteomic Analysis ◽  
Gene Content

scholarly journals Regulation of cry Gene Expression in Bacillus thuringiensis

Toxins ◽  
2014 ◽  
Vol 6 (7) ◽  
pp. 2194-2209 ◽  
Author(s):  
Chao Deng ◽  
Qi Peng ◽  
Fuping Song ◽  
Didier Lereclus
Keyword(s):  
Gene Expression ◽  
Bacillus Thuringiensis ◽  
Cry Gene
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