scholarly journals Isolation, Characterization and Crystal Morphology Study of Bacillus thuringiensis Isolates from Soils of Punjab

2018 ◽  
Vol 12 (1) ◽  
pp. 189-193 ◽  
Author(s):  
Babita Mukhija ◽  
Veena Khanna
Keyword(s):  
Bacillus Thuringiensis ◽  
Crystal Morphology ◽  
Morphology Study

Structural feature evolution – from fluids to the solid phase – and crystal morphology study of LASSBio 1601: a cyclohexyl-N-acylhydrazone derivative

RSC Advances ◽  
2015 ◽  
Vol 5 (50) ◽  
pp. 39889-39898 ◽  
Author(s):  
Fanny Nascimento Costa ◽  
Tiago F. da Silva ◽  
Eduardo Miguez B. Silva ◽  
Regina C. R. Barroso ◽  
Delson Braz ◽  
...  
Keyword(s):  
Structural Feature ◽  
Structural Characterization ◽  
Crystal Morphology ◽  
Solid Phase ◽  
Morphology Study ◽  
Feature Evolution

Synthesis and structural characterization of LASSBIO 1601: a cyclohexyl-N-acylhydrazone derivative.

Insecticidal Activity of a Cry1Ca toxin of Bacillus thuringiensis Berliner (Firmicutes: Bacillaceae) and Its Synergism with the Cyt1Aa Toxin Against Aedes aegypti (Diptera: Culicidae)

2020 ◽  
Vol 57 (6) ◽  
pp. 1852-1856
Author(s):  
Sebastian E González-Villarreal ◽  
Mónica García-Montelongo ◽  
Jorge E Ibarra
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Synergistic Effect ◽  
Joint Action ◽  
Recombinant Strain ◽  
Crystal Morphology ◽  
Graphical Method ◽  
Toxic Activity ◽  
Lepidopteran Species ◽  
Gene Coding

Abstract The Cry1C protein family of Bacillus thuringiensis form bipyramidal crystals, which are commonly associated with toxic activity against lepidopteran species; however, some members of this family may also be toxic to dipterans. In the present work, the Cry1Ca16 protein, synthesized by the B. thuringiensis LBIT-1217 strain, was analyzed. The gene coding for this protein was amplified, sequenced, and cloned into the pSTAB vector, which was electro-transferred into the acrystalliferous B. thuringiensis 4Q7 strain. The recombinant strain showed the expected bipyramidal crystal morphology, identical to the original LBIT-1217 strain and exhibited toxicity against larvae of Aedes aegypti (Diptera). Pure crystals from the recombinant strain were used in bioassays against Ae. aegypti larvae, estimating an LC50 of 4.61 μg/ml. Further studies on Cry1Ca16 mosquitocidal potential included joint-action tests with the Cyt1Aa protein crystals from B. thuringiensis israelensis. An LC50 using pure Cyt1Aa crystals was estimated at 0.73 μg/ml, whereas an LC50 of 0.61 μg/ml was estimated when both toxins were tested together. Data from these bioassays was analyzed using joint-action tests such as the Tammes-Bakuniak graphical method and the formula proposed by Tabashnik (1992). Both tests clearly showed a synergistic effect between these two toxins.

scholarly journals Diversity of Bacillus thuringiensis Strains From Qatar as Shown by Crystal Morphology, δ-Endotoxins and Cry Gene Content

2018 ◽  
Vol 9 ◽  
Author(s):  
Kavita Nair ◽  
Roda Al-Thani ◽  
Dhabia Al-Thani ◽  
Fatima Al-Yafei ◽  
Talaat Ahmed ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Crystal Morphology ◽  
Gene Content ◽  
Cry Gene

scholarly journals Detection of New cry Genes of Bacillus thuringiensis by Use of a Novel PCR Primer System

2010 ◽  
Vol 76 (18) ◽  
pp. 6150-6155 ◽  
Author(s):  
Pedro A. Noguera ◽  
Jorge E. Ibarra
Keyword(s):  
Bacillus Thuringiensis ◽  
Crystal Morphology ◽  
Protein Sequences ◽  
Open Reading Frames ◽  
Cry Genes ◽  
Coding Region ◽  
Gene Sequences ◽  
Cloning And Sequencing ◽  
Cry Protein ◽  
Reading Frames

ABSTRACT On the basis of the known cry gene sequences of Bacillus thuringiensis, three sets of primers were designed from four conserved blocks found in the delta-endotoxin-coding region. The primer pairs designed amplify the regions between blocks 1 and 5, 2 and 5, and 1 and 4. In silico analyses indicated that 100% of the known three-domain cry gene sequences can be amplified by these sets of primers. To test their ability to amplify known and unknown cry gene sequences, 27 strains from the CINVESTAV (LBIT series) collection showing atypical crystal morphology were selected. Their DNA was used as the template with the new primer system, and after a systematic amplification and sequencing of the amplicons, each strain showed one or more cry-related sequences, totaling 54 different sequences harbored by the 27 strains. Seven sequences were selected on the basis of their low level of identity to the known cry sequences, and once cloning and sequencing of the complete open reading frames were done, three new cry-type genes (primary ranks) were identified and the toxins that they encode were designated Cry57Aa1, Cry58Aa1, and Cry59Aa1 by the B. thuringiensis Toxin Nomenclature Committee. The rest of the seven sequences were classified Cry8Ka2, Cry8-like, Cry20Ba1, and Cry1Ma1 by the committee. The crystal morphology of the selected strains and analysis of the new Cry protein sequences showed interesting peculiarities.

“Preparation of Single Crystalline Gold Films for ED Studies”

1972 ◽  
Vol 30 ◽  
pp. 634-635
Author(s):  
Joseph D. C. Peng
Keyword(s):  
Crystal Morphology ◽  
Diffraction Theory ◽  
Scattering Matrix ◽  
Vacuum Evaporation ◽  
Gold Films ◽  
Matrix Formulation ◽  
Silver Films ◽  
Single Crystalline ◽  
Grating Pattern ◽  
Stacking Disorder

The relative intensities of the ED spots in a cross-grating pattern can be calculated using N-beam electron diffraction theory. The scattering matrix formulation of N-beam ED theory has been previously applied to imperfect microcrystals of gold containing stacking disorder (coherent twinning) in the (111) crystal plane. In the present experiment an effort has been made to grow single-crystalline, defect-free (111) gold films of a uniform and accurately know thickness using vacuum evaporation techniques. These represent stringent conditions to be met experimentally; however, if a meaningful comparison is to be made between theory and experiment, these factors must be carefully controlled. It is well-known that crystal morphology, perfection, and orientation each have pronounced effects on relative intensities in single crystals.The double evaporation method first suggested by Pashley was employed with some modifications. Oriented silver films of a thickness of about 1500Å were first grown by vacuum evaporation on freshly cleaved mica, with the substrate temperature at 285° C during evaporation with the deposition rate at 500-800Å/sec.

SEARCH FOR NATURAL ISOLATES OF Bacillus thuringiensis FOR DEVELOPMENT OF ECOLOGICALLY FRIENDLY BIOLOGICALS

2018 ◽  
Vol 53 (5) ◽  
pp. 1062-1069
Author(s):  
S.D. Grishechkina ◽  
◽  
V.P. Ermolova ◽  
T.A. Romanova ◽  
A.A. Nizhnikov ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Natural Isolates
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