scholarly journals AvaR1, a Butenolide-Type Autoregulator Receptor in Streptomyces avermitilis, Directly Represses Avenolide and Avermectin Biosynthesis and Multiple Physiological Responses

2017 ◽  
Vol 8 ◽  
Author(s):  
Jianya Zhu ◽  
Zhi Chen ◽  
Jilun Li ◽  
Ying Wen
Keyword(s):  
Physiological Responses ◽  
Streptomyces Avermitilis ◽  
Avermectin Biosynthesis ◽  
Autoregulator Receptor

RAPD Analysis on Avermectin High-Producing Strain of Streptomyces Avermilis Irradiated by 12C6+ Ions

2011 ◽  
Vol 339 ◽  
pp. 701-704 ◽  
Author(s):  
Yong Heng Bo ◽  
Shu Yang Wang ◽  
Ji Hong Chen ◽  
Jian Ping Liang
Keyword(s):  
Random Amplified Polymorphic Dna ◽  
Genetic Material ◽  
Similarity Index ◽  
Heavy Ion ◽  
Streptomyces Avermitilis ◽  
Original Strain ◽  
Ion Accelerator ◽  
Modern Physics ◽  
Avermectin Biosynthesis ◽  
Academy Of Sciences

Italic textAbstract. Industrial Streptomyces avermitilis were irradiated by 80 MeV/u 12C6+ ions whith were provided by the Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou Heavy Ion Accelerator. The high titers of mutants were obtained with different doses. The original strains and the high titers of mutants were detected and analyzed by Random Amplified Polymorphic DNA technology (RAPD). The results show that bands could be got from amplify the original strain by 4 primers of 28 primers and the high titers of mutants were amplified by the 4 primers. The amplified products were analysed by Jaccard formula. After 10 Gy, 30 Gy, 40 Gy, 45 Gy, 50 Gy, 70 Gy isodose irradiation, the similarity index were 0.63, 0.75, 0.71, 0.8, 0.61, 0.73. It was confirmed that at the DNA level, not only the high titers strains and the original mutant strain’s DNA were different, but also the high titers of the different mutants’ DNA were different. Therefore, ion implantation method changed the different Streptomyces avermitilis genetic material which promote the importance trace of avermectin biosynthesis changed, and different high titers of Streptomyces avermitilis were obtained.

Characterization of AvaR1, a butenolide-autoregulator receptor for biosynthesis of a Streptomyces hormone in Streptomyces avermitilis

2016 ◽  
Vol 100 (22) ◽  
pp. 9581-9591 ◽  
Author(s):  
Suandi Pratama Sultan ◽  
Shigeru Kitani ◽  
Kiyoko T Miyamoto ◽  
Hiroyuki Iguchi ◽  
Tokitaka Atago ◽  
...  
Keyword(s):  
Streptomyces Avermitilis ◽  
Autoregulator Receptor

scholarly journals The autoregulator receptor homologue AvaR3 plays a regulatory role in antibiotic production, mycelial aggregation and colony development of Streptomyces avermitilis

Microbiology ◽  
2011 ◽  
Vol 157 (8) ◽  
pp. 2266-2275 ◽  
Author(s):  
Kiyoko T. Miyamoto ◽  
Shigeru Kitani ◽  
Mamoru Komatsu ◽  
Haruo Ikeda ◽  
Takuya Nihira
Keyword(s):  
Antibiotic Production ◽  
Genomic Analysis ◽  
Morphological Differentiation ◽  
Gene Clusters ◽  
Streptomyces Avermitilis ◽  
Colony Development ◽  
Amino Acid Residues ◽  
Biosynthetic Genes ◽  
Morphological Defects ◽  
Autoregulator Receptor

The γ-butyrolactone autoregulator receptor has been shown to control secondary metabolism and/or morphological differentiation across many Streptomyces species. Streptomyces avermitilis produces an important anthelmintic agent (avermectin) and two further polyketide antibiotics, filipin and oligomycin. Genomic analysis of S. avermitilis revealed that this micro-organism has the clustered putative autoregulator receptor genes distant from the antibiotic biosynthetic gene clusters. Here, we describe the characterization of avaR3, one of the clustered receptor genes, which encodes a protein containing an extra stretch of amino acid residues that has not been found in the family of autoregulator receptors. Disruption of avaR3 resulted in markedly decreased production of avermectins, with delayed expression of avermectin biosynthetic genes, suggesting that AvaR3 positively controls the avermectin biosynthetic genes. Moreover, the disruption caused increased production of filipin without any changes in the transcriptional profile of the filipin biosynthetic genes, suggesting that filipin production is indirectly controlled by AvaR3. The avaR3 disruptant displayed fragmented growth in liquid culture and conditional morphological defects on solid medium. These findings demonstrated that AvaR3 acts as a global regulator that controls antibiotic production and cell morphology.

Activation of cryptic phthoxazolin A production in Streptomyces avermitilis by the disruption of autoregulator-receptor homologue AvaR3

2017 ◽  
Vol 124 (6) ◽  
pp. 611-617 ◽  
Author(s):  
Dian Anggraini Suroto ◽  
Shigeru Kitani ◽  
Kiyoko T. Miyamoto ◽  
Yasuko Sakihama ◽  
Masayoshi Arai ◽  
...  
Keyword(s):  
Streptomyces Avermitilis ◽  
Autoregulator Receptor

The PhoP transcription factor negatively regulates avermectin biosynthesis in Streptomyces avermitilis

2015 ◽  
Vol 99 (24) ◽  
pp. 10547-10557 ◽  
Author(s):  
Renjun Yang ◽  
Xingchao Liu ◽  
Ying Wen ◽  
Yuan Song ◽  
Zhi Chen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Streptomyces Avermitilis ◽  
Avermectin Biosynthesis
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