scholarly journals The LacI–Family Transcription Factor, RbsR, Is a Pleiotropic Regulator of Motility, Virulence, Siderophore and Antibiotic Production, Gas Vesicle Morphogenesis and Flotation in Serratia

2017 ◽  
Vol 8 ◽  
Author(s):  
Chin M. Lee ◽  
Rita E. Monson ◽  
Rachel M. Adams ◽  
George P. C. Salmond
Keyword(s):  
Transcription Factor ◽  
Antibiotic Production ◽  
Pleiotropic Regulator ◽  
Laci Family

scholarly journals AtMYB61, an R2R3-MYB transcription factor, functions as a pleiotropic regulator via a small gene network

2012 ◽  
Vol 195 (4) ◽  
pp. 774-786 ◽  
Author(s):  
Julia M. Romano ◽  
Christian Dubos ◽  
Michael B. Prouse ◽  
Olivia Wilkins ◽  
Henry Hong ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gene Network ◽  
Myb Transcription Factor ◽  
R2r3 Myb ◽  
Pleiotropic Regulator

scholarly journals Secondary nucleotide messenger c-di-GMP exerts a global control on natural product biosynthesis in streptomycetes

2020 ◽  
Vol 48 (3) ◽  
pp. 1583-1598 ◽  
Author(s):  
Roman Makitrynskyy ◽  
Olga Tsypik ◽  
Desirèe Nuzzo ◽  
Thomas Paululat ◽  
David L Zechel ◽  
...  
Keyword(s):  
Antibiotic Production ◽  
Regulatory Gene ◽  
Morphological Differentiation ◽  
Gene Clusters ◽  
Guanosine Monophosphate ◽  
Cellular Processes ◽  
Expression Of Genes ◽  
Moenomycin A ◽  
Biochemical Analyses ◽  
Pleiotropic Regulator

Abstract Cyclic dimeric 3′-5′ guanosine monophosphate, c-di-GMP, is a ubiquitous second messenger controlling diverse cellular processes in bacteria. In streptomycetes, c-di-GMP plays a crucial role in a complex morphological differentiation by modulating an activity of the pleiotropic regulator BldD. Here we report that c-di-GMP plays a key role in regulating secondary metabolite production in streptomycetes by altering the expression levels of bldD. Deletion of cdgB encoding a diguanylate cyclase in Streptomycesghanaensis reduced c-di-GMP levels and the production of the peptidoglycan glycosyltransferase inhibitor moenomycin A. In contrast to the cdgB mutant, inactivation of rmdB, encoding a phosphodiesterase for the c-di-GMP hydrolysis, positively correlated with the c-di-GMP and moenomycin A accumulation. Deletion of bldD adversely affected the synthesis of secondary metabolites in S. ghanaensis, including the production of moenomycin A. The bldD-deficient phenotype is partly mediated by an increase in expression of the pleiotropic regulatory gene wblA. Genetic and biochemical analyses demonstrate that a complex of c-di-GMP and BldD effectively represses transcription of wblA, thus preventing sporogenesis and sustaining antibiotic synthesis. These results show that manipulation of the expression of genes controlling c-di-GMP pool has the potential to improve antibiotic production as well as activate the expression of silent gene clusters.

scholarly journals Mutation in afsR Leads to A-Factor Deficiency in Streptomyces griseus B2682

2018 ◽  
Vol 28 (5) ◽  
pp. 216-224
Author(s):  
Melinda Szilágyi ◽  
Éva Márton ◽  
Dávid Lukács ◽  
Zsuzsanna Birkó ◽  
Zoltán Kele ◽  
...  
Keyword(s):  
Antibiotic Production ◽  
Shuttle Vector ◽  
Streptomyces Griseus ◽  
Extracellular Protease ◽  
Phenotypic Characterization ◽  
Gene Encoding ◽  
Factor Production ◽  
Factor Deficiency ◽  
Key Enzyme ◽  
Pleiotropic Regulator

<b><i>Background/Aims:</i></b> A-factor, a γ-butyrolactone autoregulator, in <i>Streptomyces griseus</i> is involved in the regulation of differentiation and antibiotic production. Here we studied the <i>S. griseus</i> B2682-AFN (A-factor negative) bald mutant that harbors a nonsense mutation in the <i>afsR</i> gene encoding a pleiotropic regulator. Our aim was to prove that this mutation is the cause of the A-factor deficiency in AFN. We also studied whether AfsR regulates A-factor production by AfsA, which is supposed to be the only specific key enzyme in A-factor biosynthesis. <b><i>Methods:</i></b> Wild <i>afsR</i> was cloned to the pHJL401 shuttle vector and was transformed to the <i>S. griseus</i> AFN and B2682 strains. During phenotypic characterization, sporulation, antibiotic, protease, A-factor, and AfsA protein production were studied. <b><i>Results:</i></b> Transformation of AFN by a wild <i>afsR</i> restored its phenotype including sporulation, antibiotic, extracellular protease, and A-factor production. Introduction of <i>afsR</i> to the B2682 wild-type strain resulted in antibiotic and extracellular protease overproduction that was accompanied with an elevated A-factor level. AfsA was detected both in AFN and B2682. <b><i>Conclusions:</i></b> AfsR has an effect on the regulation of A-factor production in <i>S. griseus</i>. The presence of AfsA is not sufficient for normal A-factor production. AfsR regulates A-factor biosynthesis independently of AfsA.

scholarly journals Interspecies DNA Microarray Analysis Identifies WblA as a Pleiotropic Down-Regulator of Antibiotic Biosynthesis in Streptomyces

2007 ◽  
Vol 189 (11) ◽  
pp. 4315-4319 ◽  
Author(s):  
Seung-Hoon Kang ◽  
Jianqiang Huang ◽  
Han-Na Lee ◽  
Yoon-Ah Hur ◽  
Stanley N. Cohen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Microarray Analysis ◽  
Dna Microarray ◽  
Antibiotic Production ◽  
Streptomyces Coelicolor ◽  
Morphological Differentiation ◽  
Antibiotic Biosynthesis ◽  
Gene Encoding ◽  
Putative Transcription Factor

ABSTRACT Using Streptomyces coelicolor microarrays to discover regulators of gene expression in other Streptomyces species, we identified wblA, a whiB-like gene encoding a putative transcription factor, as a down-regulator of doxorubicin biosynthesis in Streptomyces peucetius. Further analysis revealed that wblA functions pleiotropically to control antibiotic production and morphological differentiation in streptomycetes. Our results reveal a novel biological role for wblA and show the utility of interspecies microarray analysis for the investigation of streptomycete gene expression.

Transcription factor/DNA interactions visualized by electron spectroscopic imaging

1992 ◽  
Vol 50 (1) ◽  
pp. 450-451
Author(s):  
David P. Bazett-Jones ◽  
Mark L. Brown
Keyword(s):  
Transcription Factor ◽  
Dna Sequences ◽  
Transcription Initiation ◽  
Molecular Mechanisms ◽  
Phosphorus Content ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
Dna Backbone ◽  
Two Parameters ◽  
High Level

A multisubunit RNA polymerase enzyme is ultimately responsible for transcription initiation and elongation of RNA, but recognition of the proper start site by the enzyme is regulated by general, temporal and gene-specific trans-factors interacting at promoter and enhancer DNA sequences. To understand the molecular mechanisms which precisely regulate the transcription initiation event, it is crucial to elucidate the structure of the transcription factor/DNA complexes involved. Electron spectroscopic imaging (ESI) provides the opportunity to visualize individual DNA molecules. Enhancement of DNA contrast with ESI is accomplished by imaging with electrons that have interacted with inner shell electrons of phosphorus in the DNA backbone. Phosphorus detection at this intermediately high level of resolution (≈lnm) permits selective imaging of the DNA, to determine whether the protein factors compact, bend or wrap the DNA. Simultaneously, mass analysis and phosphorus content can be measured quantitatively, using adjacent DNA or tobacco mosaic virus (TMV) as mass and phosphorus standards. These two parameters provide stoichiometric information relating the ratios of protein:DNA content.

The role of transcription factor Egr‐1 in IL‐1 up‐regulation of tubular CD44 expression

Nephrology ◽  
2000 ◽  
Vol 5 (3) ◽  
pp. A92-A92
Author(s):  
Takazoe K ◽  
Foti R ◽  
Hurst La ◽  
Atkins Rc ◽  
Nikolic‐Paterson DJ.
Keyword(s):  
Transcription Factor ◽  
Cd44 Expression
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