scholarly journals Indole-Induced Activities of β-Lactamase and Efflux Pump Confer Ampicillin Resistance in Pseudomonas putida KT2440

2017 ◽  
Vol 8 ◽  
Author(s):  
Jisun Kim ◽  
Bora Shin ◽  
Chulwoo Park ◽  
Woojun Park
Keyword(s):  
Pseudomonas Putida ◽  
Efflux Pump ◽  
Pseudomonas Putida Kt2440 ◽  
Ampicillin Resistance

scholarly journals Involvement of MexS and MexEF-OprN in Resistance to Toxic Ion Chelators in Pseudomonas putida KT2440

2020 ◽  
Vol 8 (11) ◽  
pp. 1782
Author(s):  
Tania Henriquez ◽  
Tom Baldow ◽  
Yat Kei Lo ◽  
Dina Weydert ◽  
Andreas Brachmann ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Efflux Pump ◽  
Toxic Metal ◽  
Growth Defect ◽  
Metal Chelators ◽  
Pseudomonas Putida Kt2440 ◽  
Pseudomonas Species ◽  
Resistance Nodulation Division ◽  
Rnd Efflux Pump

Bacteria must be able to cope with harsh environments to survive. In Gram-negative bacteria like Pseudomonas species, resistance-nodulation-division (RND) transporters contribute to this task by pumping toxic compounds out of cells. Previously, we found that the RND system TtgABC of Pseudomonas putida KT2440 confers resistance to toxic metal chelators of the bipyridyl group. Here, we report that the incubation of a ttgB mutant in medium containing 2,2’-bipyridyl generated revertant strains able to grow in the presence of this compound. This trait was related to alterations in the pp_2827 locus (homolog of mexS in Pseudomonas aeruginosa). The deletion and complementation of pp_2827 confirmed the importance of the locus for the revertant phenotype. Furthermore, alteration in the pp_2827 locus stimulated expression of the mexEF-oprN operon encoding an RND efflux pump. Deletion and complementation of mexF confirmed that the latter system can compensate the growth defect of the ttgB mutant in the presence of 2,2’-bipyridyl. To our knowledge, this is the first report on a role of pp_2827 (mexS) in the regulation of mexEF-oprN in P. putida KT2440. The results expand the information about the significance of MexEF-OprN in the stress response of P. putida KT2440 and the mechanisms for coping with bipyridyl toxicity.

scholarly journals Mechanisms of Resistance to Chloramphenicol in Pseudomonas putida KT2440

2011 ◽  
Vol 56 (2) ◽  
pp. 1001-1009 ◽  
Author(s):  
Matilde Fernández ◽  
Susana Conde ◽  
Jesús de la Torre ◽  
Carlos Molina-Santiago ◽  
Juan-Luis Ramos ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Efflux Pump ◽  
Protein Biosynthesis ◽  
Cellular Stress Response ◽  
Knockout Mutant ◽  
Pseudomonas Putida Kt2440 ◽  
Altered Expression ◽  
Content Type ◽  
Reading Frame ◽  
A Genome

ABSTRACTPseudomonas putidaKT2440 is a chloramphenicol-resistant bacterium that is able to grow in the presence of this antibiotic at a concentration of up to 25 μg/ml. Transcriptomic analyses revealed that the expression profile of 102 genes changed in response to this concentration of chloramphenicol in the culture medium. The genes that showed altered expression include those involved in general metabolism, cellular stress response, gene regulation, efflux pump transporters, and protein biosynthesis. Analysis of a genome-wide collection of mutants showed that survival of a knockout mutant in the TtgABC resistance-nodulation-division (RND) efflux pump and mutants in the biosynthesis of pyrroloquinoline (PQQ) were compromised in the presence of chloramphenicol. The analysis also revealed that an ABC extrusion system (PP2669/PP2668/PP2667) and the AgmR regulator (PP2665) were needed for full resistance toward chloramphenicol. Transcriptional arrays revealed that AgmR controls the expression of thepqqgenes and the operon encoding the ABC extrusion pump from the promoter upstream of open reading frame (ORF) PP2669.

scholarly journals Knockout of Extracytoplasmic Function Sigma Factor ECF-10 Affects Stress Resistance and Biofilm Formation in Pseudomonas putida KT2440

2014 ◽  
Vol 80 (16) ◽  
pp. 4911-4919 ◽  
Author(s):  
Beatrix Tettmann ◽  
Andreas Dötsch ◽  
Olivier Armant ◽  
Christopher D. Fjell ◽  
Joerg Overhage
Keyword(s):  
Antibiotic Resistance ◽  
Pseudomonas Putida ◽  
Mutant Strain ◽  
Stress Resistance ◽  
Sigma Factor ◽  
Biofilm Formation ◽  
Efflux Pump ◽  
Pseudomonas Putida Kt2440 ◽  
Content Type ◽  
Extracytoplasmic Function Sigma Factor

ABSTRACTPseudomonas putidais a Gram-negative soil bacterium which is well-known for its versatile lifestyle, controlled by a large repertoire of transcriptional regulators. Besides one- and two-component regulatory systems, the genome ofP. putidareveals 19 extracytoplasmic function (ECF) sigma factors involved in the adaptation to changing environmental conditions. In this study, we demonstrate that knockout of extracytoplasmic function sigma factor ECF-10, encoded by open reading frame PP4553, resulted in 2- to 4-fold increased antibiotic resistance to quinolone, β-lactam, sulfonamide, and chloramphenicol antibiotics. In addition, the ECF-10 mutant exhibited enhanced formation of biofilms after 24 h of incubation. Transcriptome analysis using Illumina sequencing technology resulted in the detection of 12 genes differentially expressed (>2-fold) in the ECF-10 knockout mutant strain compared to their levels of expression in wild-type cells. Among the upregulated genes werettgA,ttgB, andttgC, which code for the major multidrug efflux pump TtgABC inP. putidaKT2440. Investigation of an ECF-10 andttgAdouble-knockout strain and attgABC-overexpressing strain demonstrated the involvement of efflux pump TtgABC in the stress resistance and biofilm formation phenotypes of the ECF-10 mutant strain, indicating a new role for this efflux pump beyond simple antibiotic resistance inP. putidaKT2440.

scholarly journals Genetic Analysis of Functions Involved in Adhesion of Pseudomonas putida to Seeds

2000 ◽  
Vol 182 (9) ◽  
pp. 2363-2369 ◽  
Author(s):  
Manuel Espinosa-Urgel ◽  
Amparo Salido ◽  
Juan-Luis Ramos
Keyword(s):  
Pseudomonas Putida ◽  
Calcium Binding ◽  
Efflux Pump ◽  
Protein A ◽  
Surface Proteins ◽  
Peptide Transporter ◽  
Multidrug Efflux Pump ◽  
Bacterial Populations ◽  
Abiotic Surfaces ◽  
Initial Seed

ABSTRACT Many agricultural uses of bacteria require the establishment of efficient bacterial populations in the rhizosphere, for which colonization of plant seeds often constitutes a critical first step.Pseudomonas putida KT2440 is a strain that colonizes the rhizosphere of a number of agronomically important plants at high population densities. To identify the functions involved in initial seed colonization by P. putida KT2440, we subjected this strain to transposon mutagenesis and screened for mutants defective in attachment to corn seeds. Eight different mutants were isolated and characterized. While all of them showed reduced attachment to seeds, only two had strong defects in their adhesion to abiotic surfaces (glass and different plastics). Sequences of the loci affected in all eight mutants were obtained. None of the isolated genes had previously been described in P. putida, although four of them showed clear similarities with genes of known functions in other organisms. They corresponded to putative surface and membrane proteins, including a calcium-binding protein, a hemolysin, a peptide transporter, and a potential multidrug efflux pump. One other showed limited similarities with surface proteins, while the remaining three presented no obvious similarities with known genes, indicating that this study has disclosed novel functions.

scholarly journals The exopolysaccharide gene cluster pea is transcriptionally controlled by RpoS and repressed by AmrZ in Pseudomonas putida KT2440

2019 ◽  
Vol 218 ◽  
pp. 1-11 ◽  
Author(s):  
Huizhong Liu ◽  
Huaduo Yan ◽  
Yujie Xiao ◽  
Hailing Nie ◽  
Qiaoyun Huang ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Gene Cluster ◽  
Pseudomonas Putida Kt2440

scholarly journals Conversion and assimilation of furfural and 5-(hydroxymethyl)furfural by Pseudomonas putida KT2440

2017 ◽  
Vol 4 ◽  
pp. 22-28 ◽  
Author(s):  
Michael T. Guarnieri ◽  
Mary Ann Franden ◽  
Christopher W. Johnson ◽  
Gregg T. Beckham
Keyword(s):  
Pseudomonas Putida ◽  
Pseudomonas Putida Kt2440 ◽  
Hydroxymethyl Furfural
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