scholarly journals PprM, a Cold Shock Domain-Containing Protein from Deinococcus radiodurans, Confers Oxidative Stress Tolerance to Escherichia coli

2017 ◽  
Vol 7 ◽  
Author(s):  
Sun-Ha Park ◽  
Harinder Singh ◽  
Deepti Appukuttan ◽  
Sunwook Jeong ◽  
Yong Jun Choi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Escherichia Coli ◽  
Stress Tolerance ◽  
Cold Shock ◽  
Deinococcus Radiodurans ◽  
Oxidative Stress Tolerance ◽  
Cold Shock Domain

Synechocystis Fe superoxide dismutase gene confers oxidative stress tolerance to Escherichia coli

2004 ◽  
Vol 316 (2) ◽  
pp. 540-544 ◽  
Author(s):  
Jyotirmoy Bhattacharya ◽  
Krishnarup GhoshDastidar ◽  
Anirban Chatterjee ◽  
Manoj Majee ◽  
Arun Lahiri Majumder
Keyword(s):  
Oxidative Stress ◽  
Escherichia Coli ◽  
Superoxide Dismutase ◽  
Stress Tolerance ◽  
Oxidative Stress Tolerance ◽  
Superoxide Dismutase Gene

scholarly journals The Novel ncRNA OsiR Positively Regulates Expression of katE2 and is Required for Oxidative Stress Tolerance in Deinococcus radiodurans

2020 ◽  
Vol 21 (9) ◽  
pp. 3200 ◽  
Author(s):  
Lihua Gao ◽  
Xiaonan Chen ◽  
Ye Tian ◽  
Yongliang Yan ◽  
Yuhua Zhan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Stress Tolerance ◽  
Catalase Activity ◽  
Target Genes ◽  
Deinococcus Radiodurans ◽  
Oxidative Stress Response ◽  
Sequencing Analysis ◽  
Translation Efficiency ◽  
Oxidative Stress Tolerance

Deinococcus radiodurans is a polyextremophilic bacterium well known for its extreme resistance to irradiation, oxidative stress, and other damaging conditions. Many small noncoding RNAs (ncRNAs) in D. radiodurans have been identified by deep sequencing analysis and computational predictions. However, the precise roles of ncRNAs and their target genes in the oxidative stress response have not been investigated. Here, we report the identification and characterization of a novel ncRNA named OsiR (for oxidative stress-induced ncRNA). Oxidative stress tolerance analysis showed that deleting osiR significantly decreased viability, total antioxidant capacity, and catalase activity in D. radiodurans under oxidative stress conditions. Comparative phenotypic and qRT-PCR analyses of an osiR mutant identify a role of OsiR in regulating the expression of the catalase gene katE2. Microscale thermophoresis and genetic complementation showed that a 21-nt sequence in the stem–loop structure of OsiR (204–244 nt) directly base pairs with its counterpart in the coding region of katE2 mRNA (843–866 nt) via a 19 nt region. In addition, deletion of katE2 caused a significant reduction of catalase activity and oxidative stress tolerance similar to that observed in an osiR mutant. Our results show that OsiR positively regulates oxidative stress tolerance in D. radiodurans by increasing the mRNA stability and translation efficiency of katE2. This work provides a new regulatory pathway mediated by ncRNA for the oxidative stress response that most likely contributes to the extreme tolerances of D. radiodurans.

scholarly journals Elimination of GlnKAmtB affects serine biosynthesis and improves growth and stress tolerance of Escherichia coli under nutrient-rich conditions

2020 ◽  
Vol 367 (23) ◽  
Author(s):  
Romina Frare ◽  
Margarita Stritzler ◽  
Cecilia Pascuan ◽  
Karen Liebrenz ◽  
Luisa Galindo-Sotomonte ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Escherichia Coli ◽  
Growth Rate ◽  
Stress Tolerance ◽  
Citrate Synthase ◽  
Ammonium Uptake ◽  
Uptake System ◽  
Oxidative Stress Tolerance ◽  
The Impact ◽  
Serine Biosynthesis

ABSTRACT Nitrogen is a most important nutrient resource for Escherichia coli and other bacteria that harbor the glnKamtB operon, a high-affinity ammonium uptake system highly interconnected with cellular metabolism. Although this system confers an advantage to bacteria when growing under nitrogen-limiting conditions, little is known about the impact of these genes on microbial fitness under nutrient-rich conditions. Here, the genetically tractable E. coli BW25113 strain and its glnKamtB-null mutant (JW0441) were used to analyze the impact of GlnK-AmtB on growth rates and oxidative stress tolerance. Strain JW0441 showed a shorter initial lag phase, higher growth rate, higher citrate synthase activity, higher oxidative stress tolerance and lower expression of serA than strain BW25113 under nutrient-rich conditions, suggesting a fitness cost to increase metabolic plasticity associated with serine metabolism. The overexpression of serA in strain JW0441 resulted in a decreased growth rate and stress tolerance in nutrient-rich conditions similar to that of strain BW25113, suggesting that the negative influence on bacterial fitness imposed by GlnK-AmtB can be traced to the control of serine biosynthesis. Finally, we discuss the potential applications of glnKamtB mutants in bioproduction processes.

scholarly journals Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: insights from ROS detoxification and scavenging

2015 ◽  
Vol 6 ◽  
Author(s):  
Mohammad A. Hossain ◽  
Soumen Bhattacharjee ◽  
Saed-Moucheshi Armin ◽  
Pingping Qian ◽  
Wang Xin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Stress Tolerance ◽  
Oxidative Stress Tolerance
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