scholarly journals Francisella philomiragia Infection and Lethality in Mammalian Tissue Culture Cell Models, Galleria mellonella, and BALB/c Mice

2016 ◽  
Vol 7 ◽  
Author(s):  
Crystal N. Propst ◽  
Stephanie L. Pylypko ◽  
Ryan J. Blower ◽  
Saira Ahmad ◽  
Mohammad Mansoor ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Galleria Mellonella ◽  
Culture Cell ◽  
Tissue Culture Cell ◽  
Mammalian Tissue ◽  
Cell Models

A Method for Electron Microscopic Study of Tissue Culture Cell Monolayers Grown in Tubes

1967 ◽  
Vol 25 ◽  
pp. 132-133
Author(s):  
R. Stephens ◽  
G. Schidlovsky ◽  
S. Kuzmic ◽  
P. Gaudreau
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Light Microscopy ◽  
Cell Sheet ◽  
Culture Cell ◽  
Tissue Culture Cell ◽  
Electron Microscopic ◽  
Cell Sheets ◽  
Morphological Alterations ◽  
Culture Cells

The usual method of scraping or trypsinization to detach tissue culture cell sheets from their glass substrate for further pelletization and processing for electron microscopy introduces objectionable morphological alterations. It is also impossible under these conditions to study a particular area or individual cell which have been preselected by light microscopy in the living state.Several schemes which obviate centrifugation and allow the embedding of nondetached tissue culture cells have been proposed. However, they all preserve only a small part of the cell sheet and make use of inverted gelatin capsules which are in this case difficult to handle.We have evolved and used over a period of several years a technique which allows the embedding of a complete cell sheet growing at the inner surface of a tissue culture roller tube. Observation of the same cell by light microscopy in the living and embedded states followed by electron microscopy is performed conveniently.

scholarly journals Use of Tissue Culture Cell Lines to Evaluate HIV Antiviral Resistance

2008 ◽  
Vol 24 (7) ◽  
pp. 957-967 ◽  
Author(s):  
Halina Krowicka ◽  
James E. Robinson ◽  
Rebecca Clark ◽  
Shannon Hager ◽  
Stephanie Broyles ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Cell Lines ◽  
Culture Cell ◽  
Antiviral Resistance ◽  
Tissue Culture Cell

Immunobiology of primary intracranial tumors

1983 ◽  
Vol 59 (2) ◽  
pp. 208-216 ◽  
Author(s):  
M. Stephen Mahaley ◽  
G. Yancey Gillespie ◽  
Ritchie P. Gillespie ◽  
Pamela J. Watkins ◽  
Darell D. Bigner ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Cell Line ◽  
Glioma Cell ◽  
Malignant Gliomas ◽  
Osteogenic Sarcoma ◽  
Culture Cell ◽  
Glioma Cell Line ◽  
Tissue Culture Cell ◽  
Serological Studies ◽  
Line Following

✓ Serial serological studies were carried out on 19 of 20 patients with malignant gliomas who were actively immunized with one of two human glioma tissue culture cell lines (D-54MG or U-251MG). Most patients mounted a significant serum reaction to histocompatibility antigens (HLA's), as well as an antibody response to fetal bovine serum (FBS) which was added to the glioma-cell inoculum. These two sources of antibody accounted for greater than 90% of the antibody induced by these inoculations. Two patients continued to have significant amounts of binding antibody to the original immunizing cell line following exhaustive absorptions of FBS and HLA antibodies. One of these two had all remaining significant antibody removed by further absorption of the serum against the 2-T osteogenic sarcoma tissue culture cell line known to possess antigens cross-reactive with human gliomas. One single patient continued to show significant antibody binding to the original glioma cell line following absorption against FBS, human platelets, and the 2-T cell line, and therefore seems to have produced glioma-distinctive antibodies in response to immunization. The antibody preparation from this patient was also cytotoxic against the original glioma cell line, as well as another recently cultured human glioblastoma cell line. The significance of these serological studies is discussed as it relates to immunological responses patients with gliomas may make to active immunization.

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