scholarly journals Use of Tissue Culture Cell Lines to Evaluate HIV Antiviral Resistance

2008 ◽  
Vol 24 (7) ◽  
pp. 957-967 ◽  
Author(s):  
Halina Krowicka ◽  
James E. Robinson ◽  
Rebecca Clark ◽  
Shannon Hager ◽  
Stephanie Broyles ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Cell Lines ◽  
Culture Cell ◽  
Antiviral Resistance ◽  
Tissue Culture Cell

Growth of primate and nonprimate tissue culture cell lines in X-irradiated and cortisone-treated rats

Cancer ◽  
1958 ◽  
Vol 11 (6) ◽  
pp. 1236-1241 ◽  
Author(s):  
Lewis L. Coriell ◽  
Robert M. McAllister ◽  
Bernard M. Wagner ◽  
Sheldon R. Wilson ◽  
Selena A. Dwight
Keyword(s):  
Tissue Culture ◽  
Cell Lines ◽  
Culture Cell ◽  
Tissue Culture Cell

New tissue culture cell lines derived from human squamous cell carcinoma of the cervix and vagina

1978 ◽  
Vol 130 (4) ◽  
pp. 487-496 ◽  
Author(s):  
J.C. Porter ◽  
Richard H. Nalick ◽  
Frank Vellios ◽  
William B. Neaves ◽  
P.C. MacDonald
Keyword(s):  
Tissue Culture ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Lines ◽  
Squamous Cell ◽  
Culture Cell ◽  
Tissue Culture Cell ◽  
Human Squamous Cell Carcinoma

scholarly journals Utilization of the TangoTM β-Arrestin Recruitment Technology for Cell-Based EDG Receptor Assay Development and Interrogation

2009 ◽  
Vol 14 (9) ◽  
pp. 1134-1141 ◽  
Author(s):  
Justin A. Wetter ◽  
Chetana Revankar ◽  
Bonnie J. Hanson
Keyword(s):  
Tissue Culture ◽  
Cell Lines ◽  
Culture Cell ◽  
Assay Development ◽  
Receptor Expression ◽  
Tissue Culture Cell ◽  
Functional Assay ◽  
Entire Family ◽  
Cellular Assay ◽  
Receptor Signal

Cellular assay development for the endothelial differentiation gene (EDG) family of G-protein-coupled receptors (GPCRs) and related lysophospholipid (LP) receptors is complicated by endogenous receptor expression and divergent receptor signaling. Endogenously expressed LP receptors exist in most tissue culture cell lines. these LP receptors, along with other endogenously expressed GPCRs, contribute to off-target signaling that can complicate interpretation of second-messenger-based cellular assay results. these receptors also activate a diverse and divergent set of cellular signaling pathways, necessitating the use of a variety of assay formats with mismatched procedures and functional readouts. this complicates examination and comparison of these receptors across the entire family. the tango™ technology uses the conserved β-arrestin-dependent receptor deactivation process to allow interrogation of the EDG and related receptors with a single functional assay. this method also isolates the target receptor signal, allowing the use of tissue culture cell lines regardless of their endogenous receptor expression. the authors describe the use of this technique to build cell-based receptor-specific assays for all 8 members of the EDG receptor family as well as the related LPA receptors GPR23, GPR92, and GPR87. In addition, they demonstrate the value of this technology for identification and investigation of functionally selective receptor compounds as demonstrated by the immunosuppressive compound FtY720-P and its action at the EDG1 and EDG3 receptors. ( Journal of Biomolecular Screening 2009:1134-1141)

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