scholarly journals Aire Downregulation Is Associated with Changes in the Posttranscriptional Control of Peripheral Tissue Antigens in Medullary Thymic Epithelial Cells

2016 ◽  
Vol 7 ◽  
Author(s):  
Ernna H. Oliveira ◽  
Claudia Macedo ◽  
Cristhianna V. Collares ◽  
Ana Carolina Freitas ◽  
Paula Barbim Donate ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Peripheral Tissue ◽  
Thymic Epithelial Cells ◽  
Posttranscriptional Control ◽  
Tissue Antigens ◽  
Medullary Thymic Epithelial Cells

scholarly journals miR-155 exerts posttranscriptional control of autoimmune regulator (Aire) and tissue-restricted antigen genes in medullary thymic epithelial cells

2021 ◽  
Author(s):  
Pedro P Tanaka ◽  
Ernna H Oliveira ◽  
Mayara C Machado ◽  
Max J Duarte ◽  
Amanda F Assis ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Large Scale ◽  
Interaction Analysis ◽  
Luciferase Reporter ◽  
Thymic Epithelial Cells ◽  
Posttranscriptional Control ◽  
Autoimmune Regulator ◽  
Aire Gene ◽  
Medullary Thymic Epithelial Cells

Background: The autoimmune regulator (Aire) gene is critical for the appropriate establishment of central immune tolerance. As one of the main controllers of promiscuous gene expression in the thymus, Aire promotes the expression of thousands of downstream tissue-restricted antigen (TRA) genes, cell adhesion genes and transcription factor genes in medullary thymic epithelial cells (mTECs). Despite the increasing knowledge about the role of Aire as an upstream transcriptional controller, little is known about the mechanisms by which this gene could be regulated. Results: Here, we assessed the posttranscriptional control of Aire by miRNAs. The in silico miRNA-mRNA interaction analysis predicted thermodynamically stable hybridization between the 3UTR of Aire mRNA and miR-155, which was confirmed to occur within the cellular milieu through a luciferase reporter assay. This finding enabled us to hypothesize that miR-155 might play a role as an intracellular posttranscriptional regulator of Aire mRNA. To test this hypothesis, we transfected a murine mTEC cell line with a miR-155 mimic in vitro, which reduced the mRNA and protein levels of Aire. Moreover, large-scale transcriptome analysis showed the modulation of 311 downstream mRNAs, which included 58 TRA mRNAs. Moreover, miR-155 mimic-transfected cells exhibited a decrease in their chemotaxis property compared with control thymocytes. Conclusion: Overall, the results indicate that miR-155 may posttranscriptionally control Aire mRNA as well as a crucial process by which mTECs allow migration of thymocytes through chemotaxis.

scholarly journals Lymphotoxin β Receptor Regulates the Development of CCL21-Expressing Subset of Postnatal Medullary Thymic Epithelial Cells

2013 ◽  
Vol 190 (10) ◽  
pp. 5110-5117 ◽  
Author(s):  
Enkhsaikhan Lkhagvasuren ◽  
Mie Sakata ◽  
Izumi Ohigashi ◽  
Yousuke Takahama
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Medullary Thymic Epithelial Cells ◽  
Β Receptor

scholarly journals Foxn1 Regulates Lineage Progression in Cortical and Medullary Thymic Epithelial Cells But Is Dispensable for Medullary Sublineage Divergence

PLoS Genetics ◽  
2011 ◽  
Vol 7 (11) ◽  
pp. e1002348 ◽  
Author(s):  
Craig S. Nowell ◽  
Nicholas Bredenkamp ◽  
Stéphanie Tetélin ◽  
Xin Jin ◽  
Christin Tischner ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Medullary Thymic Epithelial Cells

scholarly journals Diversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Beth Lucas ◽  
Andrea J. White ◽  
Emilie J. Cosway ◽  
Sonia M. Parnell ◽  
Kieran D. James ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Inkt Cells ◽  
Medullary Thymic Epithelial Cells

Ultrastructure of medullary thymic epithelial cells of autoimmune regulator (Aire)‐deficient mice

2009 ◽  
Vol 88 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Živana Milićević ◽  
Novica M Milićević ◽  
Martti Laan ◽  
Pärt Peterson ◽  
Kai Kisand ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Autoimmune Regulator ◽  
Medullary Thymic Epithelial Cells ◽  
Deficient Mice

scholarly journals NF-κB2 Is Required for the Control of Autoimmunity by Regulating the Development of Medullary Thymic Epithelial Cells

2006 ◽  
Vol 281 (50) ◽  
pp. 38617-38624 ◽  
Author(s):  
Baochun Zhang ◽  
Zhe Wang ◽  
Jane Ding ◽  
Pärt Peterson ◽  
William T. Gunning ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Medullary Thymic Epithelial Cells
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