Expression profile of peripheral tissue antigen genes in medullary thymic epithelial cells (mTECs) is dependent on mRNA levels of autoimmune regulator (Aire)

Immunobiology ◽  
2013 ◽  
Vol 218 (1) ◽  
pp. 96-104 ◽  
Author(s):  
Ernna H. Oliveira ◽  
Claudia Macedo ◽  
Paula B. Donate ◽  
Renata S. Almeida ◽  
Nicole Pezzi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Expression Profile ◽  
Peripheral Tissue ◽  
Thymic Epithelial Cells ◽  
Mrna Levels ◽  
Tissue Antigen ◽  
Autoimmune Regulator ◽  
Medullary Thymic Epithelial Cells

Ultrastructure of medullary thymic epithelial cells of autoimmune regulator (Aire)‐deficient mice

2009 ◽  
Vol 88 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Živana Milićević ◽  
Novica M Milićević ◽  
Martti Laan ◽  
Pärt Peterson ◽  
Kai Kisand ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Autoimmune Regulator ◽  
Medullary Thymic Epithelial Cells ◽  
Deficient Mice

Autoimmune regulator (Aire) controls the expression of microRNAs in medullary thymic epithelial cells

Immunobiology ◽  
2013 ◽  
Vol 218 (4) ◽  
pp. 554-560 ◽  
Author(s):  
Claudia Macedo ◽  
Adriane F. Evangelista ◽  
Márcia M. Marques ◽  
Shirlei Octacílio-Silva ◽  
Eduardo A. Donadi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Autoimmune Regulator ◽  
Medullary Thymic Epithelial Cells

scholarly journals Autoimmune regulator act in synergism with thymocyte adhesion in the control of lncRNAs in medullary thymic epithelial cells

2021 ◽  
Author(s):  
Max J Duarte ◽  
Romario S Mascarenhas ◽  
Amanda F Assis ◽  
Pedro P Tanaka ◽  
Cesar A Speck-Hernandez ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Model Systems ◽  
Thymic Epithelial Cells ◽  
Large Set ◽  
Rna Pol Ii ◽  
Pol Ii ◽  
Autoimmune Regulator ◽  
Medullary Thymic Epithelial Cells

The autoimmune regulator (Aire) gene in medullary thymic epithelial cells (mTECs) encodes the AIRE protein, which interacts with its partners within the nucleus. This Aire complex induces stalled RNA Pol II on chromatin to proceed with transcription elongation of a large set of messenger RNAs and microRNAs. Considering that RNA Pol II also transcribes long noncoding RNAs (lncRNAs), we hypothesized that Aire might be implicated in the upstream control of this RNA species. To test this, we employed a loss-of-function approach in which Aire knockout mTECs were compared to Aire wild-type mTECs for lncRNA transcriptional profiling both in vitro and in vivo model systems. RNA sequencing enables the differential expression profiling of lncRNAs when these cells adhere in vitro to thymocytes or do not adhere to them as a way to test the effect of cell adhesion. Sets of lncRNAs that are unique and that are shared in vitro and in vivo were identified. Among these, we found the Aire-dependent lncRNAs as for example, Platr28, Ifi30, Morrbid, Malat1, and Xist. This finding represents the first evidence that Aire mediates the transcription of lncRNAs in mTECs. Microarray hybridizations enabled us to observe that temporal thymocyte adhesion modulates the expression levels of such lncRNAs as Morrbid, Xist, and Fbxl12o after 36h of adhesion. This finding shows the existence of a synergistic mechanism involving a link between thymocyte adhesion, Aire, and lncRNAs in mTECs that might be important for immune self-representation.

scholarly journals miR-155 exerts posttranscriptional control of autoimmune regulator (Aire) and tissue-restricted antigen genes in medullary thymic epithelial cells

2021 ◽  
Author(s):  
Pedro P Tanaka ◽  
Ernna H Oliveira ◽  
Mayara C Machado ◽  
Max J Duarte ◽  
Amanda F Assis ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Large Scale ◽  
Interaction Analysis ◽  
Luciferase Reporter ◽  
Thymic Epithelial Cells ◽  
Posttranscriptional Control ◽  
Autoimmune Regulator ◽  
Aire Gene ◽  
Medullary Thymic Epithelial Cells

Background: The autoimmune regulator (Aire) gene is critical for the appropriate establishment of central immune tolerance. As one of the main controllers of promiscuous gene expression in the thymus, Aire promotes the expression of thousands of downstream tissue-restricted antigen (TRA) genes, cell adhesion genes and transcription factor genes in medullary thymic epithelial cells (mTECs). Despite the increasing knowledge about the role of Aire as an upstream transcriptional controller, little is known about the mechanisms by which this gene could be regulated. Results: Here, we assessed the posttranscriptional control of Aire by miRNAs. The in silico miRNA-mRNA interaction analysis predicted thermodynamically stable hybridization between the 3UTR of Aire mRNA and miR-155, which was confirmed to occur within the cellular milieu through a luciferase reporter assay. This finding enabled us to hypothesize that miR-155 might play a role as an intracellular posttranscriptional regulator of Aire mRNA. To test this hypothesis, we transfected a murine mTEC cell line with a miR-155 mimic in vitro, which reduced the mRNA and protein levels of Aire. Moreover, large-scale transcriptome analysis showed the modulation of 311 downstream mRNAs, which included 58 TRA mRNAs. Moreover, miR-155 mimic-transfected cells exhibited a decrease in their chemotaxis property compared with control thymocytes. Conclusion: Overall, the results indicate that miR-155 may posttranscriptionally control Aire mRNA as well as a crucial process by which mTECs allow migration of thymocytes through chemotaxis.

scholarly journals Identification of embryonic precursor cells that differentiate into thymic epithelial cells expressing autoimmune regulator

2016 ◽  
Vol 213 (8) ◽  
pp. 1441-1458 ◽  
Author(s):  
Nobuko Akiyama ◽  
Nobukazu Takizawa ◽  
Maki Miyauchi ◽  
Hiromi Yanai ◽  
Ryosuke Tateishi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tumor Necrosis Factor Receptor ◽  
Nuclear Factor Κb ◽  
Thymic Epithelial Cells ◽  
Nuclear Factor ◽  
Autoimmune Regulator ◽  
Medullary Thymic Epithelial Cells ◽  
Β Receptor ◽  
Rank Signaling

Medullary thymic epithelial cells (mTECs) expressing autoimmune regulator (Aire) are critical for preventing the onset of autoimmunity. However, the differentiation program of Aire-expressing mTECs (Aire+ mTECs) is unclear. Here, we describe novel embryonic precursors of Aire+ mTECs. We found the candidate precursors of Aire+ mTECs (pMECs) by monitoring the expression of receptor activator of nuclear factor-κB (RANK), which is required for Aire+ mTEC differentiation. pMECs unexpectedly expressed cortical TEC molecules in addition to the mTEC markers UEA-1 ligand and RANK and differentiated into mTECs in reaggregation thymic organ culture. Introduction of pMECs in the embryonic thymus permitted long-term maintenance of Aire+ mTECs and efficiently suppressed the onset of autoimmunity induced by Aire+ mTEC deficiency. Mechanistically, pMECs differentiated into Aire+ mTECs by tumor necrosis factor receptor-associated factor 6-dependent RANK signaling. Moreover, nonclassical nuclear factor-κB activation triggered by RANK and lymphotoxin-β receptor signaling promoted pMEC induction from progenitors exhibiting lower RANK expression and higher CD24 expression. Thus, our findings identified two novel stages in the differentiation program of Aire+ mTECs.

scholarly journals The Cytokine RANKL Produced by Positively Selected Thymocytes Fosters Medullary Thymic Epithelial Cells that Express Autoimmune Regulator

Immunity ◽  
2008 ◽  
Vol 29 (3) ◽  
pp. 438-450 ◽  
Author(s):  
Yu Hikosaka ◽  
Takeshi Nitta ◽  
Izumi Ohigashi ◽  
Kouta Yano ◽  
Naozumi Ishimaru ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Autoimmune Regulator ◽  
Medullary Thymic Epithelial Cells
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