scholarly journals Activation of the Human Angiotensin-(1-12)-Chymase Pathway in Rats With Human Angiotensinogen Gene Transcripts

2019 ◽  
Vol 6 ◽  
Author(s):  
Carlos M. Ferrario ◽  
Jessica VonCannon ◽  
Sarfaraz Ahmad ◽  
Kendra N. Wright ◽  
Drew J. Roberts ◽  
...  
Keyword(s):  
Angiotensinogen Gene ◽  
Gene Transcripts

scholarly journals Glucocorticoids Induce the Accumulation of Novel Angiotensinogen Gene Transcripts

1989 ◽  
Vol 264 (22) ◽  
pp. 13074-13079
Author(s):  
E T Ben-Ari ◽  
K R Lynch ◽  
J C Garrison
Keyword(s):  
Angiotensinogen Gene ◽  
Gene Transcripts

Detection of Nucleic Acids in Cells or Tissue Sections Using In situ Polymerase Chain Reaction (PCR)

1996 ◽  
Vol 54 ◽  
pp. 16-17
Author(s):  
J. R. Hully ◽  
K. R. Luehrsen ◽  
K. Aoyagi ◽  
C. Shoemaker ◽  
R. Abramson
Keyword(s):  
Nucleic Acids ◽  
Viral Infections ◽  
Anatomical Location ◽  
Rt Pcr ◽  
Reverse Transcription Pcr ◽  
Cellular Source ◽  
Gene Transcripts ◽  
Initial Description ◽  
In Cells

The development of PCR technology has greatly accelerated medical research at the genetic and molecular levels. Until recently, the inherent sensitivity of this technique has been limited to isolated preparations of nucleic acids which lack or at best have limited morphological information. With the obvious exception of cell lines, traditional PCR or reverse transcription-PCR (RT-PCR) cannot identify the cellular source of the amplified product. In contrast, in situ hybridization (ISH) by definition, defines the anatomical location of a gene and/or it’s product. However, this technique lacks the sensitivity of PCR and cannot routinely detect less than 10 to 20 copies per cell. Consequently, the localization of rare transcripts, latent viral infections, foreign or altered genes cannot be identified by this technique. In situ PCR or in situ RT-PCR is a combination of the two techniques, exploiting the sensitivity of PCR and the anatomical definition provided by ISH. Since it’s initial description considerable advances have been made in the application of in situ PCR, improvements in protocols, and the development of hardware dedicated to in situ PCR using conventional microscope slides. Our understanding of the importance of viral latency or viral burden in regards to HIV, HPV, and KSHV infections has benefited from this technique, enabling detection of single viral copies in cells or tissue otherwise thought to be normal. Clearly, this technique will be useful tool in pathobiology especially carcinogenesis, gene therapy and manipulations, the study of rare gene transcripts, and forensics.

Angiotensinogen gene polymorphisms in IDDM patients with diabetic nephropathy

Diabetes ◽  
1996 ◽  
Vol 45 (3) ◽  
pp. 367-369 ◽  
Author(s):  
L. Tarnow ◽  
F. Cambien ◽  
P. Rossing ◽  
F. S. Nielsen ◽  
B. V. Hansen ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Gene Polymorphisms ◽  
Angiotensinogen Gene

Association of Variants in Critical Core Promoter Element of Angiotensinogen Gene With Increased Risk of Essential Hypertension in Japanese

Hypertension ◽  
1997 ◽  
Vol 30 (3) ◽  
pp. 321-325 ◽  
Author(s):  
Noriyuki Sato ◽  
Tomohiro Katsuya ◽  
Hiromi Rakugi ◽  
Seiju Takami ◽  
Yukiko Nakata ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
Core Promoter ◽  
Promoter Element ◽  
Core Promoter Element ◽  
Angiotensinogen Gene ◽  
Increased Risk

scholarly journals Possible Epigenetic Origin of a Recurrent Gynandromorph Pattern in Megachile Wild Bees

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 437
Author(s):  
Daniele Sommaggio ◽  
Giuseppe Fusco ◽  
Marco Uliana ◽  
Alessandro Minelli
Keyword(s):  
Current Knowledge ◽  
Sex Differentiation ◽  
General Pattern ◽  
Body Parts ◽  
Wild Bees ◽  
Male And Female ◽  
Female Phenotype ◽  
In The Wild ◽  
Gene Transcripts ◽  
Individual Body

Gynandromorphs, i.e., individuals with a mix of male and female traits, are common in the wild bees of the genus Megachile (Hymenoptera, Apoidea). We described new transverse gynandromorphs in Megachile pilidens Alfkeen, 1924 and analyze the spatial distribution of body parts with male vs. female phenotype hitherto recorded in the transverse gynandromorphs of the genus Megachile. We identified 10 different arrangements, nine of which are minor variants of a very general pattern, with a combination of male and female traits largely shared by the gynandromorphs recorded in 20 out of 21 Megachile species in our dataset. Based on the recurrence of the same gynandromorph pattern, the current knowledge on sex determination and sex differentiation in the honey bee, and the results of recent gene-knockdown experiments in these insects, we suggest that these composite phenotypes are possibly epigenetic, rather than genetic, mosaics, with individual body parts of either male or female phenotype according to the locally expressed product of the alternative splicing of sex-determining gene transcripts.

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