scholarly journals A Salmonella Regulator Modulates Intestinal Colonization and Use of Phosphonoacetic Acid

2017 ◽  
Vol 7 ◽  
Author(s):  
Johanna R. Elfenbein ◽  
Leigh A. Knodler ◽  
Allison R. Schaeffer ◽  
Franziska Faber ◽  
Andreas J. Bäumler ◽  
...  
Keyword(s):  
Intestinal Colonization ◽  
Phosphonoacetic Acid

scholarly journals MetR-Regulated Vibrio cholerae Metabolism Is Required for Virulence

mBio ◽  
2012 ◽  
Vol 3 (5) ◽  
Author(s):  
Ryan W. Bogard ◽  
Bryan W. Davies ◽  
John J. Mekalanos
Keyword(s):  
Amino Acid ◽  
Vibrio Cholerae ◽  
Virulence Factor ◽  
Current Knowledge ◽  
Intestinal Colonization ◽  
Wild Type ◽  
Pathogenic Potential ◽  
Content Type ◽  
Mouse Intestine

ABSTRACTLysR-type transcriptional regulators (LTTRs) are the largest, most diverse family of prokaryotic transcription factors, with regulatory roles spanning metabolism, cell growth and division, and pathogenesis. Using a sequence-defined transposon mutant library, we screened a panel ofV. choleraeEl Tor mutants to identify LTTRs required for host intestinal colonization. Surprisingly, out of 38 LTTRs, only one severely affected intestinal colonization in the suckling mouse model of cholera: the methionine metabolism regulator, MetR. Genetic analysis of genes influenced by MetR revealed thatglyA1andmetJwere also required for intestinal colonization. Chromatin immunoprecipitation of MetR and quantitative reverse transcription-PCR (qRT-PCR) confirmed interaction with and regulation ofglyA1, indicating that misregulation ofglyA1is likely responsible for the colonization defect observed in themetRmutant. TheglyA1mutant was auxotrophic for glycine but exhibited wild-type trimethoprim sensitivity, making folate deficiency an unlikely cause of its colonization defect. MetJ regulatory mutants are not auxotrophic but are likely altered in the regulation of amino acid-biosynthetic pathways, including those for methionine, glycine, and serine, and this misregulation likely explains its colonization defect. However, mutants defective in methionine, serine, and cysteine biosynthesis exhibited wild-type virulence, suggesting that these amino acids can be scavenged in vivo. Taken together, our results suggest that glycine biosynthesis may be required to alleviate an in vivo nutritional restriction in the mouse intestine; however, additional roles for glycine may exist. Irrespective of the precise nature of this requirement, this study illustrates the importance of pathogen metabolism, and the regulation thereof, as a virulence factor.IMPORTANCEVibrio choleraecontinues to be a severe cause of morbidity and mortality in developing countries. Identification ofV. choleraefactors critical to disease progression offers the potential to develop or improve upon therapeutics and prevention strategies. To increase the efficiency of virulence factor discovery, we employed a regulator-centric approach to multiplex our in vivo screening capabilities and allow whole regulons inV. choleraeto be interrogated for pathogenic potential. We identified MetR as a new virulence regulator and serine hydroxymethyltransferase GlyA1 as a new MetR-regulated virulence factor, both required byV. choleraeto colonize the infant mouse intestine. Bacterial metabolism is a prerequisite to virulence, and current knowledge of in vivo metabolism of pathogens is limited. Here, we expand the known role of amino acid metabolism and regulation in virulence and offer new insights into the in vivo metabolic requirements ofV. choleraewithin the mouse intestine.

scholarly journals Methyl-donor supplementation prevents intestinal colonization by Adherent-Invasive E. coli in a mouse model of Crohn’s disease

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Elodie Gimier ◽  
Mélissa Chervy ◽  
Allison Agus ◽  
Adeline Sivignon ◽  
Elisabeth Billard ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Mouse Model ◽  
Intestinal Colonization ◽  
Methyl Donor ◽  
E Coli

Intestinal colonization, microbiota, and probiotics

2006 ◽  
Vol 149 (5) ◽  
pp. S115-S120 ◽  
Author(s):  
Seppo Salminen ◽  
Erika Isolauri
Keyword(s):  
Intestinal Colonization

Structural studies of oriented zirconium bis(phosphonoacetic acid) using solid-state phosphorus-31 and carbon-13 NMR

1992 ◽  
Vol 114 (11) ◽  
pp. 4144-4150 ◽  
Author(s):  
David A. Burwell ◽  
Kathleen G. Valentine ◽  
Jozef H. Timmermans ◽  
Mark E. Thompson
Keyword(s):  
Solid State ◽  
Structural Studies ◽  
Phosphonoacetic Acid

Escherichia coliNissle 1917 for probiotic use in piglets: evidence for intestinal colonization

2009 ◽  
Vol 107 (5) ◽  
pp. 1697-1710 ◽  
Author(s):  
S. Barth ◽  
S. Duncker ◽  
J. Hempe ◽  
G. Breves ◽  
G. Baljer ◽  
...  
Keyword(s):  
Intestinal Colonization
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