scholarly journals Transcriptome analysis of Vibrio parahaemolyticus in type III secretion system 1 inducing conditions

2014 ◽  
Vol 4 ◽  
Author(s):  
Seth D. Nydam ◽  
Devendra H. Shah ◽  
Douglas R. Call
Keyword(s):  
Transcriptome Analysis ◽  
Type Iii Secretion ◽  
Vibrio Parahaemolyticus ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Type Iii ◽  
System 1

scholarly journals Regulatory actions of ToxR and CalR on their own genes and type III secretion system 1 in Vibrio parahaemolyticus

Oncotarget ◽  
2017 ◽  
Vol 8 (39) ◽  
pp. 65809-65822 ◽  
Author(s):  
George Osei-Adjei ◽  
He Gao ◽  
Ying Zhang ◽  
Lingyu Zhang ◽  
Wenhui Yang ◽  
...  
Keyword(s):  
Type Iii Secretion ◽  
Vibrio Parahaemolyticus ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Type Iii ◽  
System 1 ◽  
Regulatory Actions

Type III secretion system 1 of Vibrio parahaemolyticus induces oncosis in both epithelial and monocytic cell lines

Microbiology ◽  
2009 ◽  
Vol 155 (3) ◽  
pp. 837-851 ◽  
Author(s):  
Xiaohui Zhou ◽  
Michael E. Konkel ◽  
Douglas R. Call
Keyword(s):  
Cell Death ◽  
Cell Lines ◽  
Type Iii Secretion ◽  
Vibrio Parahaemolyticus ◽  
Active Form ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Monocytic Cell ◽  
Type Iii ◽  
System 1

The Vibrio parahaemolyticus type III secretion system 1 (T3SS1) induces cytotoxicity in mammalian epithelial cells. We characterized the cell death phenotype in both epithelial (HeLa) and monocytic (U937) cell lines following infection with V. parahaemolyticus. Using a combination of the wild-type strain and gene knockouts, we confirmed that V. parahaemolyticus strain NY-4 was able to induce cell death in both cell lines via a T3SS1-dependent mechanism. Bacterial contact, but not internalization, was required for T3SS1-induced cytotoxicity. The mechanism of cell death involves formation of a pore structure on the surface of infected HeLa and U937 cells, as demonstrated by cellular swelling, uptake of cell membrane-impermeable dye and protection of cytotoxicity by osmoprotectant (PEG3350). Western blot analysis showed that poly ADP ribose polymerase (PARP) was not cleaved and remained in its full-length active form. This result was evident for seven different V. parahaemolyticus strains. V. parahaemolyticus-induced cytotoxicity was not inhibited by addition of the pan-caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK) or the caspase-1 inhibitor N-acetyl-tyrosyl-valyl-alanyl-aspartyl-aldehyde (Ac-YVAD-CHO); thus, caspases were not involved in T3SS1-induced cytotoxicity. DNA fragmentation was not evident following infection and autophagic vacuoles were not observed after monodansylcadaverine staining. We conclude that T3SS1 of V. parahaemolyticus strain NY-4 induces a host cell death primarily via oncosis rather than apoptosis, pyroptosis or autophagy.

scholarly journals The small RNA Spot 42 regulates the expression of the type III secretion system 1 (T3SS1) chaperone protein VP1682 inVibrio parahaemolyticus

2015 ◽  
Vol 362 (21) ◽  
pp. fnv173 ◽  
Author(s):  
Tomotaka Tanabe ◽  
Katsushiro Miyamoto ◽  
Hiroshi Tsujibo ◽  
Shigeo Yamamoto ◽  
Tatsuya Funahashi
Keyword(s):  
Small Rna ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Type Iii ◽  
Chaperone Protein ◽  
System 1

scholarly journals In Macrophages, Caspase-1 Activation by SopE and the Type III Secretion System-1 of S. Typhimurium Can Proceed in the Absence of Flagellin

PLoS ONE ◽  
2010 ◽  
Vol 5 (8) ◽  
pp. e12477 ◽  
Author(s):  
Claudia Hoffmann ◽  
Marlies Galle ◽  
Sabrina Dilling ◽  
Rina Käppeli ◽  
Andreas J. Müller ◽  
...  
Keyword(s):  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Type Iii ◽  
Caspase 1 ◽  
System 1
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