Functional cloning of Vibrio parahaemolyticus type III secretion system 1 in Escherichia coli K-12 strain as a molecular syringe

2012 ◽  
Vol 427 (2) ◽  
pp. 242-247 ◽  
Author(s):  
Yukihiro Akeda ◽  
Tomomi Kimura ◽  
Aiko Yamasaki ◽  
Toshio Kodama ◽  
Tetsuya Iida ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Type Iii Secretion ◽  
Vibrio Parahaemolyticus ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Type Iii ◽  
K 12 ◽  
System 1

scholarly journals The ETT2 Gene Cluster, Encoding a Second Type III Secretion System from Escherichia coli, Is Present in the Majority of Strains but Has Undergone Widespread Mutational Attrition

2004 ◽  
Vol 186 (11) ◽  
pp. 3547-3560 ◽  
Author(s):  
Chuan-Peng Ren ◽  
Roy R. Chaudhuri ◽  
Amanda Fivian ◽  
Christopher M. Bailey ◽  
Martin Antonio ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Cluster ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Genome Sequences ◽  
Phylogenetic Structure ◽  
E Coli ◽  
Type Iii ◽  
K 12

ABSTRACT ETT2 is a second cryptic type III secretion system in Escherichia coli which was first discovered through the analysis of genome sequences of enterohemorrhagic E. coli O157:H7. Comparative analyses of Escherichia and Shigella genome sequences revealed that the ETT2 gene cluster is larger than was previously thought, encompassing homologues of genes from the Spi-1, Spi-2, and Spi-3 Salmonella pathogenicity islands. ETT2-associated genes, including regulators and chaperones, were found at the same chromosomal location in the majority of genome-sequenced strains, including the laboratory strain K-12. Using a PCR-based approach, we constructed a complete tiling path through the ETT2 gene cluster for 79 strains, including the well-characterized E. coli reference collection supplemented with additional pathotypes. The ETT2 gene cluster was found to be present in whole or in part in the majority of E. coli strains, whether pathogenic or commensal, with patterns of distribution and deletion mirroring the known phylogenetic structure of the species. In almost all strains, including enterohemorrhagic E. coli O157:H7, ETT2 has been subjected to varying degrees of mutational attrition that render it unable to encode a functioning secretion system. A second type III secretion system-associated locus that likely encodes the ETT2 translocation apparatus was found in some E. coli strains. Intact versions of both ETT2-related clusters are apparently present in enteroaggregative E. coli strain O42.

scholarly journals Regulatory actions of ToxR and CalR on their own genes and type III secretion system 1 in Vibrio parahaemolyticus

Oncotarget ◽  
2017 ◽  
Vol 8 (39) ◽  
pp. 65809-65822 ◽  
Author(s):  
George Osei-Adjei ◽  
He Gao ◽  
Ying Zhang ◽  
Lingyu Zhang ◽  
Wenhui Yang ◽  
...  
Keyword(s):  
Type Iii Secretion ◽  
Vibrio Parahaemolyticus ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Type Iii ◽  
System 1 ◽  
Regulatory Actions

Type III secretion system 1 of Vibrio parahaemolyticus induces oncosis in both epithelial and monocytic cell lines

Microbiology ◽  
2009 ◽  
Vol 155 (3) ◽  
pp. 837-851 ◽  
Author(s):  
Xiaohui Zhou ◽  
Michael E. Konkel ◽  
Douglas R. Call
Keyword(s):  
Cell Death ◽  
Cell Lines ◽  
Type Iii Secretion ◽  
Vibrio Parahaemolyticus ◽  
Active Form ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Monocytic Cell ◽  
Type Iii ◽  
System 1

The Vibrio parahaemolyticus type III secretion system 1 (T3SS1) induces cytotoxicity in mammalian epithelial cells. We characterized the cell death phenotype in both epithelial (HeLa) and monocytic (U937) cell lines following infection with V. parahaemolyticus. Using a combination of the wild-type strain and gene knockouts, we confirmed that V. parahaemolyticus strain NY-4 was able to induce cell death in both cell lines via a T3SS1-dependent mechanism. Bacterial contact, but not internalization, was required for T3SS1-induced cytotoxicity. The mechanism of cell death involves formation of a pore structure on the surface of infected HeLa and U937 cells, as demonstrated by cellular swelling, uptake of cell membrane-impermeable dye and protection of cytotoxicity by osmoprotectant (PEG3350). Western blot analysis showed that poly ADP ribose polymerase (PARP) was not cleaved and remained in its full-length active form. This result was evident for seven different V. parahaemolyticus strains. V. parahaemolyticus-induced cytotoxicity was not inhibited by addition of the pan-caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK) or the caspase-1 inhibitor N-acetyl-tyrosyl-valyl-alanyl-aspartyl-aldehyde (Ac-YVAD-CHO); thus, caspases were not involved in T3SS1-induced cytotoxicity. DNA fragmentation was not evident following infection and autophagic vacuoles were not observed after monodansylcadaverine staining. We conclude that T3SS1 of V. parahaemolyticus strain NY-4 induces a host cell death primarily via oncosis rather than apoptosis, pyroptosis or autophagy.

scholarly journals Escherichia coli type III secretion system 2 (ETT2) is widely distributed in avian pathogenic Escherichia coli isolates from Eastern China

2016 ◽  
Vol 144 (13) ◽  
pp. 2824-2830 ◽  
Author(s):  
S. WANG ◽  
X. LIU ◽  
X. XU ◽  
Y. ZHAO ◽  
D. YANG ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Type Iii Secretion ◽  
Bacterial Infections ◽  
Eastern China ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Secretion Systems ◽  
E Coli ◽  
Type Iii ◽  
System 2

SUMMARYPathogens utilize type III secretion systems to deliver effector proteins, which facilitate bacterial infections. The Escherichia coli type III secretion system 2 (ETT2) which plays a crucial role in bacterial virulence, is present in the majority of E. coli strains, although ETT2 has undergone widespread mutational attrition. We investigated the distribution and characteristics of ETT2 in avian pathogenic E. coli (APEC) isolates and identified five different ETT2 isoforms, including intact ETT2, in 57·6% (141/245) of the isolates. The ETT2 locus was present in the predominant APEC serotypes O78, O2 and O1. All of the ETT2 loci in the serotype O78 isolates were degenerate, whereas an intact ETT2 locus was mostly present in O1 and O2 serotype strains, which belong to phylogenetic groups B2 and D, respectively. Interestingly, a putative second type III secretion-associated locus (eip locus) was present only in the isolates with an intact ETT2. Moreover, ETT2 was more widely distributed in APEC isolates and exhibited more isoforms compared to ETT2 in human extraintestinal pathogenic E. coli, suggesting that APEC might be a potential risk to human health. However, there was no distinct correlation between ETT2 and other virulence factors in APEC.

scholarly journals A pH-dependent conformational change in EspA, a component of the Escherichia coli O157:H7 type III secretion system

FEBS Journal ◽  
2005 ◽  
Vol 272 (11) ◽  
pp. 2773-2783 ◽  
Author(s):  
Tomoaki Kato ◽  
Daizo Hamada ◽  
Takashi Fukui ◽  
Makoto Hayashi ◽  
Takeshi Honda ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Conformational Change ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Escherichia Coli O157 ◽  
Type Iii ◽  
Ph Dependent
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