scholarly journals The Promise of Bacteriophage Therapy for Burkholderia cepacia Complex Respiratory Infections

2012 ◽  
Vol 1 ◽  
Author(s):  
Diana D. Semler ◽  
Karlene H. Lynch ◽  
Jonathan J. Dennis
Keyword(s):  
Burkholderia Cepacia ◽  
Respiratory Infections ◽  
Burkholderia Cepacia Complex ◽  
Bacteriophage Therapy

scholarly journals Experimental Bacteriophage Therapy Increases Survival of Galleria mellonella Larvae Infected with Clinically Relevant Strains of the Burkholderia cepacia Complex

2009 ◽  
Vol 53 (5) ◽  
pp. 2205-2208 ◽  
Author(s):  
Kimberley D. Seed ◽  
Jonathan J. Dennis
Keyword(s):  
Alternative Treatment ◽  
Burkholderia Cepacia ◽  
Phage Therapy ◽  
Galleria Mellonella ◽  
Respiratory Infections ◽  
Burkholderia Cepacia Complex ◽  
Infection Model ◽  
Bacteriophage Therapy ◽  
Antibiotic Resistant

ABSTRACT The Burkholderia cepacia complex (BCC) is a group of bacterial pathogens that are highly antibiotic resistant and associated with debilitating respiratory infections. Although bacteriophages of the BCC have been isolated and characterized, no studies have yet examined phage therapy against the BCC in vivo. In a caterpillar infection model, we show that BCC phage therapy is an alternative treatment possibility and is highly effective under specific conditions.

scholarly journals Aerosol Phage Therapy Efficacy in Burkholderia cepacia Complex Respiratory Infections

2014 ◽  
Vol 58 (7) ◽  
pp. 4005-4013 ◽  
Author(s):  
Diana D. Semler ◽  
Amanda D. Goudie ◽  
Warren H. Finlay ◽  
Jonathan J. Dennis
Keyword(s):  
Intraperitoneal Injection ◽  
Burkholderia Cepacia ◽  
Phage Therapy ◽  
Respiratory Infections ◽  
Burkholderia Cepacia Complex ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Content Type ◽  
Therapy Efficacy

ABSTRACTPhage therapy has been suggested as a potential treatment for highly antibiotic-resistant bacteria, such as the species of theBurkholderia cepaciacomplex (BCC). To address this hypothesis, experimentalB. cenocepaciarespiratory infections were established in mice using a nebulizer and a nose-only inhalation device. Following infection, the mice were treated with one of fiveB. cenocepacia-specific phages delivered as either an aerosol or intraperitoneal injection. The bacterial and phage titers within the lungs were assayed 2 days after treatment, and mice that received the aerosolized phage therapy demonstrated significant decreases in bacterial loads. Differences in phage activity were observedin vivo. Mice that received phage treatment by intraperitoneal injection did not demonstrate significantly reduced bacterial loads, although phage particles were isolated from their lung tissue. Based on these data, aerosol phage therapy appears to be an effective method for treating highly antibiotic-resistant bacterial respiratory infections, including those caused by BCC bacteria.

scholarly journals Thinking Outside the Bug: Targeting Outer Membrane Proteins for Burkholderia Vaccines

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 495
Author(s):  
Megan E. Grund ◽  
Jeon Soo ◽  
Christopher K. Cote ◽  
Rita Berisio ◽  
Slawomir Lukomski
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Burkholderia Cepacia ◽  
Respiratory Infections ◽  
Outer Membrane Proteins ◽  
Burkholderia Cepacia Complex ◽  
Resistant Bacteria ◽  
Respiratory Pathogens ◽  
Significant Treatment ◽  
Gram Negative

Increasing antimicrobial resistance due to misuse and overuse of antimicrobials, as well as a lack of new and innovative antibiotics in development has become an alarming global threat. Preventative therapeutics, like vaccines, are combative measures that aim to stop infections at the source, thereby decreasing the overall use of antibiotics. Infections due to Gram-negative pathogens pose a significant treatment challenge because of substantial multidrug resistance that is acquired and spread throughout the bacterial population. Burkholderia spp. are Gram-negative intrinsically resistant bacteria that are responsible for environmental and nosocomial infections. The Burkholderia cepacia complex are respiratory pathogens that primarily infect immunocompromised and cystic fibrosis patients, and are acquired through contaminated products and equipment, or via patient-to-patient transmission. The Burkholderia pseudomallei complex causes percutaneous wound, cardiovascular, and respiratory infections. Transmission occurs through direct exposure to contaminated water, water-vapors, or soil, leading to the human disease melioidosis, or the equine disease glanders. Currently there is no licensed vaccine against any Burkholderia pathogen. This review will discuss Burkholderia vaccine candidates derived from outer membrane proteins, OmpA, OmpW, Omp85, and Bucl8, encompassing their structures, conservation, and vaccine formulation.

scholarly journals Current Advances in Burkholderia Vaccines Development

Cells ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 2671
Author(s):  
Guanbo Wang ◽  
Paulina Zarodkiewicz ◽  
Miguel A. Valvano
Keyword(s):  
Burkholderia Cepacia ◽  
Burkholderia Pseudomallei ◽  
Vaccine Development ◽  
Respiratory Infections ◽  
Bacterial Species ◽  
Antimicrobial Treatment ◽  
Burkholderia Cepacia Complex ◽  
Attractive Alternative ◽  
Wide Range ◽  
Substantial Progress

The genus Burkholderia includes a wide range of Gram-negative bacterial species some of which are pathogenic to humans and other vertebrates. The most pathogenic species are Burkholderia mallei, Burkholderia pseudomallei, and the members of the Burkholderia cepacia complex (Bcc). B. mallei and B. pseudomallei, the cause of glanders and melioidosis, respectively, are considered potential bioweapons. The Bcc comprises a subset of Burkholderia species associated with respiratory infections in people with chronic granulomatous disease and cystic fibrosis. Antimicrobial treatment of Burkholderia infections is difficult due to the intrinsic multidrug antibiotic resistance of these bacteria; prophylactic vaccines provide an attractive alternative to counteract these infections. Although commercial vaccines against Burkholderia infections are still unavailable, substantial progress has been made over recent years in the development of vaccines against B. pseudomallei and B. mallei. This review critically discusses the current advances in vaccine development against B. mallei, B. pseudomallei, and the Bcc.

scholarly journals Comparison of Microbiological Characteristics and Genetic Diversity between Burkholderia cepacia Complex Isolates from Vascular Access and Other Clinical Infections

2020 ◽  
Vol 9 (1) ◽  
pp. 51
Author(s):  
Min Yi Wong ◽  
Yuan-Hsi Tseng ◽  
Tsung-Yu Huang ◽  
Bor-Shyh Lin ◽  
Chun-Wu Tung ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Vascular Access ◽  
Burkholderia Cepacia ◽  
Bacterial Species ◽  
Diversity Indices ◽  
Burkholderia Cepacia Complex ◽  
Reca Gene ◽  
Clinical Effects ◽  
Environmental Distribution ◽  
Microbiological Characteristics

Burkholderia cepacia complex (BCC) is a group of closely related bacteria with widespread environmental distribution. BCC bacteria are opportunistic pathogens that cause nosocomial infections in patients, especially cystic fibrosis (CF). Multilocus sequence typing (MLST) is used nowadays to differentiate species within the BCC complex. This study collected 41 BCC isolates from vascular access infections (VAIs) and other clinical infections between 2014 and 2020. We preliminarily identified bacterial isolates using standard biochemical procedures and further conducted recA gene sequencing and MLST for species identification. We determined genetic diversity indices using bioinformatics software. We studied 14 isolates retrieved from patients with VAIs and observed that Burkholderia cepacia was the predominant bacterial species, and B. contaminans followed by B. cenocepacia were mainly retrieved from patients with other infections. According to MLST data, we identified that all B. contaminans isolates belonged to ST102, while a wide variety of sequence types (STs) were found in B. cenocepacia isolates. In summary, the high diversity and easy transmission of BCC increase BCC infections, which provides insights into their potential clinical effects in non-CF infections.

Multi-institutional outbreak of Burkholderia cepacia complex associated with contaminated mannitol solution prepared in compounding pharmacy

2013 ◽  
Vol 41 (11) ◽  
pp. 1038-1042 ◽  
Author(s):  
Maria Beatriz Souza Dias ◽  
Larissa G.T. Cavassin ◽  
Valeska Stempliuk ◽  
Luciene S. Xavier ◽  
Renata D. Lobo ◽  
...  
Keyword(s):  
Burkholderia Cepacia ◽  
Burkholderia Cepacia Complex ◽  
Mannitol Solution
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