scholarly journals Exosomes From Adipose-Derived Stem Cells: The Emerging Roles and Applications in Tissue Regeneration of Plastic and Cosmetic Surgery

2020 ◽  
Vol 8 ◽  
Author(s):  
Mingchen Xiong ◽  
Qi Zhang ◽  
Weijie Hu ◽  
Chongru Zhao ◽  
Wenchang Lv ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tissue Regeneration ◽  
Cosmetic Surgery ◽  
Adipose Derived Stem Cells

Development of a cell delivery system using alginate microbeads for tissue regeneration

2016 ◽  
Vol 4 (20) ◽  
pp. 3515-3525 ◽  
Author(s):  
Shirae K. Leslie ◽  
Anthony M. Nicolini ◽  
Gobalakrishnan Sundaresan ◽  
Jamal Zweit ◽  
Barbara D. Boyan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Delivery System ◽  
Tissue Regeneration ◽  
Cell Delivery ◽  
Adipose Derived Stem Cells ◽  
Viable Cells ◽  
A Cell

Alginate microbeads incorporating adipose-derived stem cells (ASCs) have potential for delivering viable cells capable of facilitating tissue regeneration.

Periodontal Tissue Regeneration with Adipose-Derived Stem Cells

2007 ◽  
pp. 110306233438005 ◽  
Author(s):  
Morikuni Tobita ◽  
A. Cagri Uysal ◽  
Rei Ogawa ◽  
Hiko Hyakusoku ◽  
Hiroshi Mizuno
Keyword(s):  
Stem Cells ◽  
Tissue Regeneration ◽  
Adipose Derived Stem Cells ◽  
Periodontal Tissue ◽  
Periodontal Tissue Regeneration

scholarly journals Adipose-derived stem cells increase angiogenesis through matrix metalloproteinase-dependent collagen remodeling

2016 ◽  
Vol 8 (2) ◽  
pp. 205-215 ◽  
Author(s):  
Young Hye Song ◽  
Seung Hee Shon ◽  
Mengrou Shan ◽  
Abraham D Stroock ◽  
Claudia Fischbach
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Matrix Metalloproteinase ◽  
Tissue Regeneration ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Adipose Derived Stem Cells ◽  
Collagen Remodeling

Adipose-derived stem cells (ASCs) are key regulators of extracellular matrix remodeling that modulates neovascularization during tissue regeneration.

282 PORCINE ADIPOSE-DERIVED STEM CELLS ARE INDUCED TOWARD NEUROGENIC LINEAGES BY CELL-TO-CELL INTERACTIONS BUT NOT BY SOLUBLE FACTORS RELEASED BY NEURONS ISOLATED FROM ADULT AND FETAL BRAIN

2013 ◽  
Vol 25 (1) ◽  
pp. 289
Author(s):  
K. C. S. Roballo ◽  
A. C. M. Ercolin ◽  
M. Bionaz ◽  
C. E. Ambrosio ◽  
M. B. Wheeler
Keyword(s):  
Stem Cells ◽  
Conditioned Medium ◽  
Tissue Regeneration ◽  
Cell Interactions ◽  
Adult Brain ◽  
Physical Contact ◽  
Adipose Derived Stem Cells ◽  
Neuronal Markers ◽  
The Brain

Stroke, Parkinson’s, Alzheimer’s, and other neurological diseases that are relatively frequent in human involve loss of neurons. The advent of tissue regeneration using stem cells holds great promise in finding cures. In particular, mesenchymal stem cells (MSC) appear to be a very potent source for tissue regeneration. Among MSC subtypes, adipose-derived stem cells (ASC) have several distinct advantages. The ASC are abundant, are easy to isolate and expand in vitro, can be used for heterologous as well autologous transplants, and have multilineage differentiation capacity. In addition to osteocytes, chondrocytes, and adipocytes, the ASC have been successfully differentiated into neuronal-like cells by addition of specific neurogenic factors. However, in vivo differentiation of ASC into neurons remains to be demonstrated. In the present study, we used an in vitro system in order to evaluate whether ASC can be induced towards neurogenic lineages by physical contact with freshly isolated neurons or by factors released by neurons without addition of specific neurogenic factors. Experimentally, ASC and neurons (NEU) were extracted from the back fat or the brain, respectively, of a boar transgenic for green fluorescent protein (GFP) or from wild type pigs. The non-GFP neurons were isolated from the brain of 32-day fetuses or adult pigs. Cells were cultivated in 24-well plates with the following combinations: only ASC or NEU in DMEM (controls), ASC with conditioned medium from NEU, or ASC+NEU. Cells were harvested at 24 h and at 3, 7, 14, and 21 days and fixed with 4% paraformaldehyde in PBS for 15 min for immunohistochemistry analysis. After fixation, neuronal differentiation was evaluated by histological staining with specific neuronal markers. The proportion of ASC that differentiated into neuronal-like cells was determined using fluorescence microscopy. We observed little proliferation of ASC in conditioned medium compared with control ASC; however, a few cells exhibited neuronal-like morphology but with no expression of neuronal markers. When ASC were co-cultured with fetal NEU, starting at 3 days, we observed, using microscope analyses, that 4 to 12% of the ASC had neuronal-like morphology and expressed neuron-associated cell markers. When ASC were co-cultured with neurons from adult brain, we observed a lower fraction (between 1 and 2%) of neuronal differentiated cells starting at 7 days. Our data are preliminary but provide evidence that when ASC are in physical contact with neurons (i.e. by cell-to-cell interactions), they can be induced to differentiate into neuronal-like cells. Further, the differentiation is more rapid and extensive when the ASC are in direct contact with fetal neurons. However, further study is necessary to determine whether these neuronal-like cells are functional neurons. In this regard, we are performing electrophysiological analysis and measurement of expression of neuronal genes. In addition, flow cytometry will be used to quantify the proportion of differentiated ASC.

scholarly journals Adipose-Derived Stem Cells in Tissue Regeneration: A Review

2013 ◽  
Vol 2013 ◽  
pp. 1-35 ◽  
Author(s):  
Patricia Zuk
Keyword(s):  
Stem Cells ◽  
Los Angeles ◽  
Tissue Regeneration ◽  
Adult Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Induced Pluripotent Cells ◽  
Initial Description ◽  
Induced Pluripotent

In 2001, researchers at the University of California, Los Angeles, described the isolation of a new population of adult stem cells from liposuctioned adipose tissue. These stem cells, now known as adipose-derived stem cells or ADSCs, have gone on to become one of the most popular adult stem cells populations in the fields of stem cell research and regenerative medicine. As of today, thousands of research and clinical articles have been published using ASCs, describing their possible pluripotency in vitro, their uses in regenerative animal models, and their application to the clinic. This paper outlines the progress made in the ASC field since their initial description in 2001, describing their mesodermal, ectodermal, and endodermal potentials both in vitro and in vivo, their use in mediating inflammation and vascularization during tissue regeneration, and their potential for reprogramming into induced pluripotent cells.

scholarly journals The synthetic artificial stem cell (SASC): Shifting the paradigm of cell therapy in regenerative engineering

2022 ◽  
Vol 119 (2) ◽  
pp. e2116865118
Author(s):  
Shiv Shah ◽  
Caldon Jayson Esdaille ◽  
Maumita Bhattacharjee ◽  
Ho-Man Kan ◽  
Cato T. Laurencin
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Therapeutic Strategy ◽  
Cartilage Degeneration ◽  
Biomechanical Properties ◽  
Adipose Derived Stem Cells ◽  
Rodent Models

Stem cells are of great interest in tissue regeneration due to their ability to modulate the local microenvironment by secreting bioactive factors (collectively, secretome). However, secretome delivery through conditioned media still requires time-consuming cell isolation and maintenance and also may contain factors antagonistic to targeted tissue regeneration. We have therefore engineered a synthetic artificial stem cell (SASC) system which mimics the paracrine effect of the stem cell secretome and provides tailorability of the composition for targeted tissue regeneration. We report the first of many applications of the SASC system we have formulated to treat osteoarthritis (OA). Choosing growth factors important to chondrogenesis and encapsulating respective recombinant proteins in poly (lactic-coglycolic acid) 85:15 (PLGA) we fabricated the SASC system. We compared the antiinflammatory and chondroprotective effects of SASC to that of adipose-derived stem cells (ADSCs) using in vitro interleukin 1B-induced and in vivo collagenase-induced osteoarthritis rodent models. We have designed SASC as an injectable therapy with controlled release of the formulated secretome. In vitro, SASC showed significant antiinflammatory and chondroprotective effects as seen by the up-regulation of SOX9 and reduction of nitric oxide, ADAMTS5, and PRG4 genes compared to ADSCs. In vivo, treatment with SASC and ADSCs significantly attenuated cartilage degeneration and improved the biomechanical properties of the articular cartilage in comparison to OA control. This SASC system demonstrates the feasibility of developing a completely synthetic, tailorable stem cell secretome which reinforces the possibility of developing a new therapeutic strategy that provides better control over targeted tissue engineering applications.

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