scholarly journals Adipose-derived stem cells increase angiogenesis through matrix metalloproteinase-dependent collagen remodeling

2016 ◽  
Vol 8 (2) ◽  
pp. 205-215 ◽  
Author(s):  
Young Hye Song ◽  
Seung Hee Shon ◽  
Mengrou Shan ◽  
Abraham D Stroock ◽  
Claudia Fischbach
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Matrix Metalloproteinase ◽  
Tissue Regeneration ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Adipose Derived Stem Cells ◽  
Collagen Remodeling

Adipose-derived stem cells (ASCs) are key regulators of extracellular matrix remodeling that modulates neovascularization during tissue regeneration.

scholarly journals Matrix metalloproteinase system is involved in cardiac extracellular matrix remodeling during pregnancy and postpartum (1152.1)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Jose Sonanez‐Organis ◽  
Sarai Limon‐Miranda ◽  
Diana Salazar‐Enriquez ◽  
Jesus Muniz‐Murguia ◽  
Enrique Sanchez‐Pastor ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Metalloproteinase ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Pregnancy And Postpartum

scholarly journals Dm1-MMP, a Matrix Metalloproteinase fromDrosophilawith a Potential Role in Extracellular Matrix Remodeling during Neural Development

2000 ◽  
Vol 275 (46) ◽  
pp. 35978-35985 ◽  
Author(s):  
Elena Llano ◽  
Alberto M. Pendás ◽  
Pedro Aza-Blanc ◽  
Thomas B. Kornberg ◽  
Carlos López-Otı́n
Keyword(s):  
Extracellular Matrix ◽  
Matrix Metalloproteinase ◽  
Neural Development ◽  
Potential Role ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling

scholarly journals An In Vitro Study on Extracellular Vesicles From Adipose-Derived Mesenchymal Stem Cells in Protecting Stress Urinary Incontinence Through MicroRNA-93/F3 Axis

2021 ◽  
Vol 12 ◽  
Author(s):  
Lu Wang ◽  
Yali Wang ◽  
Yuancui Xiang ◽  
Jinping Ma ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Extracellular Vesicles ◽  
Satellite Cells ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Collagen Iii ◽  
Primary Fibroblasts

Since the potential roles of extracellular vesicles secreted by adipose-derived mesenchymal stem cells (ADSCs) are not well understood in collagen metabolism, the purpose of this research was to evaluate the effects of ADSCs-extracellular vesicles in stress urinary incontinence and the regulatory mechanism of delivered microRNA-93 (miR-93). ADSCs were isolated and cultured, and ADSCs-extracellular vesicles were extracted and identified. Stress urinary incontinence primary fibroblasts or satellite cells were treated with ADSCs-extracellular vesicles to detect the expression of Elastin, Collagen I, and Collagen III in fibroblasts and Pax7 and MyoD in satellite cells. After transfecting ADSCs with miR-93 mimics or inhibitors, extracellular vesicles were isolated and treated with stress urinary incontinence primary fibroblasts or satellite cells to observe cell function changes. The online prediction and luciferase activity assay confirmed the targeting relationship between miR-93 and coagulation factor III (F3). The rescue experiment verified the role of ADSCs-extracellular vesicles carrying miR-93 in stress urinary incontinence primary fibroblasts and satellite cells by targeting F3. ADSCs-extracellular vesicles treatment upregulated expression of Elastin, Collagen I, and Collagen III in stress urinary incontinence primary fibroblasts and expression of Pax7 and MyoD in stress urinary incontinence primary satellite cells. miR-93 expression was increased in stress urinary incontinence primary fibroblasts or satellite cells treated with ADSCs-extracellular vesicles. Extracellular vesicles secreted by ADSCs could deliver miR-93 to fibroblasts and then negatively regulate F3 expression; ADSCs-extracellular vesicles could reverse the effect of F3 on extracellular matrix remodeling in stress urinary incontinence fibroblasts. miR-93 expression was also increased in stress urinary incontinence primary satellite cells treated by ADSCs-extracellular vesicles. Extracellular vesicles secreted by ADSCs were delivered to satellite cells through miR-93, which directly targets F3 expression and upregulates Pax7 and MyoD expression in satellite cells. Our study indicates that miR-93 delivered by ADSCs-extracellular vesicles could regulate extracellular matrix remodeling of stress urinary incontinence fibroblasts and promote activation of stress urinary incontinence satellite cells through targeting F3.

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