scholarly journals Focused Ultrasound Stimulates ER Localized Mechanosensitive PANNEXIN-1 to Mediate Intracellular Calcium Release in Invasive Cancer Cells

2020 ◽  
Vol 8 ◽  
Author(s):  
Nan Sook Lee ◽  
Chi Woo Yoon ◽  
Qing Wang ◽  
Sunho Moon ◽  
Kweon Mo Koo ◽  
...  
Keyword(s):  
Intracellular Calcium ◽  
Cancer Cells ◽  
Focused Ultrasound ◽  
Calcium Release ◽  
Invasive Cancer ◽  
Pannexin 1 ◽  
Intracellular Calcium Release

scholarly journals Focused ultrasound stimulates ER localized mechanosensitive PANNEXIN-1 to mediate intracellular calcium release in invasive cancer cells

2020 ◽  
Author(s):  
Nan Sook Lee ◽  
Chi Woo Yoon ◽  
Qing Wang ◽  
Sunho Moon ◽  
Kweon Mo Koo ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Calcium Signaling ◽  
Cancer Cells ◽  
Focused Ultrasound ◽  
Calcium Release ◽  
Atp Release ◽  
Invasive Cancer ◽  
Great Promise ◽  
Pannexin 1 ◽  
Cellular Processes

ABSTRACTFocused ultrasound (FUS) is a rapidly developing stimulus technology with the potential to uncover novel mechanosensory dependent cellular processes. Since it is noninvasive, it holds great promise for future therapeutic applications in patients used either alone or as a complement to boost existing treatments. For example, FUS stimulation causes invasive but not noninvasive cancer cell lines to exhibit marked activation of calcium signaling pathways. Here, we identify the membrane channel PANNEXIN1 (PANX1) as a mediator for activation of calcium signaling in invasive cancer cells. Knockdown of PANX1 decreases calcium signaling in invasive cells, while PANX1 overexpression enhances calcium elevations in non-invasive cancer cells. We demonstrate that FUS may directly stimulate mechanosensory PANX1 localized in endoplasmic reticulum to evoke calcium release from internal stores. This process does not depend on mechanosensory stimulus transduction through an intact cytoskeleton and does not depend on plasma membrane localized PANX1. Plasma membrane localized PANX1 however plays a different role in mediating the spread of intercellular calcium waves via ATP release. Additionally, we show that FUS stimulation evokes cytokine/chemokine release from invasive cancer cells, suggesting that FUS could be an important new adjuvant treatment to improve cancer immunotherapy.

scholarly journals Orientia tsutsugamushi Inhibits Apoptosis of Macrophages by Retarding Intracellular Calcium Release

2002 ◽  
Vol 70 (8) ◽  
pp. 4692-4696 ◽  
Author(s):  
Mee-Kyung Kim ◽  
Seung-Yong Seong ◽  
Ju-Young Seoh ◽  
Tae-Hee Han ◽  
Hyeon-Je Song ◽  
...  
Keyword(s):  
Intracellular Calcium ◽  
Calcium Concentration ◽  
Calcium Release ◽  
Cytosolic Calcium ◽  
Orientia Tsutsugamushi ◽  
Cytosolic Calcium Concentration ◽  
Infected Cells ◽  
Intracellular Calcium Release ◽  
Calcium Redistribution ◽  
In Cells

ABSTRACT Orientia tsutsugamushi shows both pro- and antiapoptotic activities in infected vertebrate cells. Apoptosis of THP-1 cells induced by beauvericin was inhibited by O. tsutsugamushi infection. Beauvericin-induced calcium redistribution was significantly reduced and retarded in cells infected with O. tsutsugamushi. Antiapoptotic activities of O. tsutsugamushi in infected cells are most probably due to inhibition of the increase in the cytosolic calcium concentration.

scholarly journals A Membrane Estrogen Receptor Mediates Intracellular Calcium Release in Astrocytes

Endocrinology ◽  
2004 ◽  
Vol 145 (8) ◽  
pp. 3788-3795 ◽  
Author(s):  
Victor V. Chaban ◽  
Alexander J. Lakhter ◽  
Paul Micevych
Keyword(s):  
Estrogen Receptor ◽  
Intracellular Calcium ◽  
Calcium Release ◽  
Intracellular Calcium Release

scholarly journals N-terminus oligomerization is conserved in intracellular calcium release channels

2014 ◽  
Vol 459 (2) ◽  
pp. 265-273 ◽  
Author(s):  
Spyros Zissimopoulos ◽  
Jason Marsh ◽  
Laurence Stannard ◽  
Monika Seidel ◽  
F. Anthony Lai
Keyword(s):  
Structure Function ◽  
Intracellular Calcium ◽  
Calcium Release ◽  
Function Parameter ◽  
Calcium Release Channels ◽  
Cellular Processes ◽  
N Terminus ◽  
Intracellular Calcium Release ◽  
Ca2 Channels

Intracellular Ca2+ channels are of paramount importance for numerous cellular processes. In the present paper we report on a novel N-terminus intersubunit interaction, an essential structure–function parameter, which is conserved in both families of intracellular Ca2+ channels.

scholarly journals Molecular mechanism of defective intracellular calcium release in heart failure and lethal arrhythmia

2012 ◽  
Vol 140 (6) ◽  
pp. 250-254
Author(s):  
Masafumi Yano ◽  
Takeshi Yamamoto ◽  
Shigeki Kobayashi ◽  
Masunori Matsuzaki
Keyword(s):  
Heart Failure ◽  
Intracellular Calcium ◽  
Molecular Mechanism ◽  
Calcium Release ◽  
Lethal Arrhythmia ◽  
Intracellular Calcium Release
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