scholarly journals Expanding the Neuron's Calcium Signaling Repertoire: Intracellular Calcium Release via Voltage-Induced PLC and IP3R Activation

PLoS Biology ◽  
2007 ◽  
Vol 5 (4) ◽  
pp. e66 ◽  
Author(s):  
Stefanie Ryglewski ◽  
Hans J Pflueger ◽  
Carsten Duch
Keyword(s):  
Calcium Signaling ◽  
Intracellular Calcium ◽  
Calcium Release ◽  
Intracellular Calcium Release

Intraventricular Hemorrhage Induces Rapid Calcium Signaling and Transcriptional Changes in the Developing Choroid Plexus

Neurosurgery ◽  
2019 ◽  
Vol 66 (Supplement_1) ◽  
Author(s):  
Cameron Sadegh ◽  
Huixin Xu ◽  
Frederick Shipley ◽  
Neil Dani ◽  
Maria K Lehtinen
Keyword(s):  
Epithelial Cells ◽  
Calcium Signaling ◽  
Intracellular Calcium ◽  
Choroid Plexus ◽  
Calcium Imaging ◽  
Intraventricular Hemorrhage ◽  
Calcium Release ◽  
Transcriptional Analysis ◽  
Transgenic Mouse Line ◽  
Intracellular Calcium Release

Abstract INTRODUCTION Intraventricular hemorrhage (IVH) leads to acute hydrocephalus in a subset of preterm infants by unclear mechanisms that potentially include increased cerebrospinal fluid (CSF) production by the choroid plexus (ChP), based on recent work in adult rats (Karimy et al., 2017). The earliest physiologic changes in ChP epithelial cells likely involve calcium signaling, which is a known cellular messenger in other secretory epithelial cell populations. The following experiments utilize rapid calcium imaging to evaluate how calcium signaling is regulated in embryonic ChP explants, and additionally determine specific transcriptional alterations in Vivo. METHODS 1) Calcium imaging of embryonic explants: Lateral ventricle ChP is dissected at embryonic day 14.5 (E14.5) from a conditional transgenic mouse line (FoxJ1-Cre; Gcamp6f). The ChP explants are kept viable with artificial CSF, exposed to focal blood injections, and imaged for calcium responses using epifluorescence and two-photon imaging. 2) Transcriptional analysis in a model of preterm IVH: Quantitative RT-PCR is performed on acutely dissected ChP at 24 and 48 hr, following in utero injection of age-matched blood in the lateral ventricles of individual embryos (E14.5 pregnant CD1 mice). RESULTS Upon exposure to age-matched E14.5 plasma, nearly all ChP epithelial cells rapidly release highly concentrated intracellular calcium followed by a mosaic pattern of recurrent calcium release. Using different conditions of calcium blockade, we identify endoplasmic reticulum storage as the primary source of intracellular calcium release. Expression of NKCC1 (the predominant apical ion co-transporter in the ChP) rises by 24 hr, suggesting increased ChP ion permeability, consistent with prior reports of a CSF hypersecretion phenotype. CONCLUSION IVH causes immediate intracellular calcium release by the ChP epithelia, followed by increased NKCC1 transcription within 24 hr. Together, these data suggest a rapid and dynamic embryonic ChP responsiveness to hemorrhage and contributes to our understanding of IVH-induced hydrocephalus pathophysiology.

scholarly journals Orientia tsutsugamushi Inhibits Apoptosis of Macrophages by Retarding Intracellular Calcium Release

2002 ◽  
Vol 70 (8) ◽  
pp. 4692-4696 ◽  
Author(s):  
Mee-Kyung Kim ◽  
Seung-Yong Seong ◽  
Ju-Young Seoh ◽  
Tae-Hee Han ◽  
Hyeon-Je Song ◽  
...  
Keyword(s):  
Intracellular Calcium ◽  
Calcium Concentration ◽  
Calcium Release ◽  
Cytosolic Calcium ◽  
Orientia Tsutsugamushi ◽  
Cytosolic Calcium Concentration ◽  
Infected Cells ◽  
Intracellular Calcium Release ◽  
Calcium Redistribution ◽  
In Cells

ABSTRACT Orientia tsutsugamushi shows both pro- and antiapoptotic activities in infected vertebrate cells. Apoptosis of THP-1 cells induced by beauvericin was inhibited by O. tsutsugamushi infection. Beauvericin-induced calcium redistribution was significantly reduced and retarded in cells infected with O. tsutsugamushi. Antiapoptotic activities of O. tsutsugamushi in infected cells are most probably due to inhibition of the increase in the cytosolic calcium concentration.

scholarly journals A Membrane Estrogen Receptor Mediates Intracellular Calcium Release in Astrocytes

Endocrinology ◽  
2004 ◽  
Vol 145 (8) ◽  
pp. 3788-3795 ◽  
Author(s):  
Victor V. Chaban ◽  
Alexander J. Lakhter ◽  
Paul Micevych
Keyword(s):  
Estrogen Receptor ◽  
Intracellular Calcium ◽  
Calcium Release ◽  
Intracellular Calcium Release

scholarly journals N-terminus oligomerization is conserved in intracellular calcium release channels

2014 ◽  
Vol 459 (2) ◽  
pp. 265-273 ◽  
Author(s):  
Spyros Zissimopoulos ◽  
Jason Marsh ◽  
Laurence Stannard ◽  
Monika Seidel ◽  
F. Anthony Lai
Keyword(s):  
Structure Function ◽  
Intracellular Calcium ◽  
Calcium Release ◽  
Function Parameter ◽  
Calcium Release Channels ◽  
Cellular Processes ◽  
N Terminus ◽  
Intracellular Calcium Release ◽  
Ca2 Channels

Intracellular Ca2+ channels are of paramount importance for numerous cellular processes. In the present paper we report on a novel N-terminus intersubunit interaction, an essential structure–function parameter, which is conserved in both families of intracellular Ca2+ channels.

scholarly journals Molecular mechanism of defective intracellular calcium release in heart failure and lethal arrhythmia

2012 ◽  
Vol 140 (6) ◽  
pp. 250-254
Author(s):  
Masafumi Yano ◽  
Takeshi Yamamoto ◽  
Shigeki Kobayashi ◽  
Masunori Matsuzaki
Keyword(s):  
Heart Failure ◽  
Intracellular Calcium ◽  
Molecular Mechanism ◽  
Calcium Release ◽  
Lethal Arrhythmia ◽  
Intracellular Calcium Release
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