Retroelement Insertion in a CRISPR/Cas9 Editing Site in the Early Embryo Intensifies Genetic Mosaicism

Cell division in eucaryotes depends on coordinated changes in nuclear and cytoskeletal components. In Drosophila melanogaster embryos, the first 13 nuclear divisions occur without cytokinesis. During the final four divisions, nuclei divide in a uniform monolayer at the surface of the embryo. These surface divisions are accompanied by dramatic changes in cortical actin and microtubule structure (Karr and Alberts, 1986), and inhibitor studies indicate that these changes are essential to orderly mitosis (Zalokar and Erk, 1976). Because the early embryo is syncytial, fluorescent probes introduced by microinjection are incorporated in structures associated with all of the nuclei in the blastoderm. In addition, the nuclei divide synchronously every 10 to 20 min. These characteristics make the syncytial blastoderm embryo an excellent system for the analysis of mitotic reorganization of both nuclear and cytoskeletal elements. However, the Drosophila embryo is a large cell, and resolution of cytoskeletal filaments and nuclear structure is hampered by out-of focus signal.

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Significantly higher pregnancy rate after transfer of EEVA (Early Embryo Viability Assessment) positive embryos in fresh IVF / ICSI-cycles

10.1055/s-0040-1717676 ◽

2020 ◽

Author(s):

L. Sela ◽

D. Hladik ◽

N. Rogenhofer ◽

S. Mahner ◽

C.J. Thaler ◽

...

Keyword(s):

Pregnancy Rate ◽

Early Embryo ◽

Embryo Viability ◽

Viability Assessment

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Diabetes alters the epigenetic landscape of the oocyte and early embryo

Reproduction Abstracts ◽

10.1530/repabs.1.p019 ◽

2014 ◽

Author(s):

Hannah M Brown ◽

Melissa A White ◽

Laura A Frank ◽

Jeremy G Thompson

Keyword(s):

Early Embryo ◽

Epigenetic Landscape

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Autism spectrum disorder in a patient with genetic mosaicism: case report

Revista Debates em Psiquiatria ◽

10.25118/2236-918x-7-5-5 ◽

2017 ◽

Vol Ano 7 ◽

pp. 38-41

Author(s):

Ana Sofia Pontes Trillo ◽

Mariana Gianola Arruda ◽

Camila Fernandes Bonifácio Jubara ◽

Isabela Mosconi Caldas ◽

Sonia Maria Motta Palma

Keyword(s):

Autism Spectrum Disorder ◽

Case Report ◽

Autism Spectrum ◽

Spectrum Disorder ◽

Genetic Mosaicism

O presente relato descreve o caso de um paciente com transtorno do espectro autista (TEA) associado ao mosaicismo genético 46XY, uma condição rara e pouco relatada. Os autores descrevem a evolução do paciente e discutem a literatura sobre anomalias cromossônicas associadas ao TEA. Conclui-se enfatizando que a avaliação clínica de cada caso de TEA deveria contemplar sempre aspectos neurológicos, psiquiátricos e genéticos.

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