scholarly journals Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo

2014 ◽  
Vol 2 ◽  
Author(s):  
Yoshinori Makino ◽  
Erina Inoue ◽  
Masashi Hada ◽  
Keisuke Aoshima ◽  
Satsuki Kitano ◽  
...  
Keyword(s):  
Mouse Line ◽  
Reporter Mouse ◽  
Dual Color

scholarly journals Live imaging YAP signaling in vivo with fusion reporter mice

2021 ◽  
Author(s):  
Bin Gu ◽  
Brian Bradshaw ◽  
Min Zhu ◽  
Yu Sun ◽  
Sevan Hopyan ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Cell Polarity ◽  
Near Infrared ◽  
Kinase Activity ◽  
Live Imaging ◽  
Mouse Line ◽  
Reporter Mouse ◽  
Reporter Mice ◽  
Mammalian Embryonic Development

YAP protein is a critical regulator of mammalian embryonic development. By generating a near-infrared fusion YAP reporter mouse line, we have achieved high-resolution live imaging of YAP localization during mouse embryonic development. We have validated the reporter by demonstrating its predicted responses to blocking Lats kinase activity or blocking cell polarity. The YAP fusion reporter mice and imaging methods will open new opportunities for understanding dynamic YAP signaling in vivo in many different situations.

scholarly journals The mito::mKate2 mouse: A far-red fluorescent reporter mouse line for tracking mitochondrial dynamics in vivo

genesis ◽  
2017 ◽  
Vol 56 (2) ◽  
pp. e23087 ◽  
Author(s):  
Anthony P. Barrasso ◽  
Xuefei Tong ◽  
Ross A. Poché
Keyword(s):  
Mitochondrial Dynamics ◽  
Mouse Line ◽  
Fluorescent Reporter ◽  
Reporter Mouse

scholarly journals A Dual-Color Bioluminescence Reporter Mouse for Simultaneous in vivo Imaging of T Cell Localization and Function

2019 ◽  
Vol 9 ◽  
Author(s):  
Jan Willem Kleinovink ◽  
Laura Mezzanotte ◽  
Giorgia Zambito ◽  
Marieke F. Fransen ◽  
Luis J. Cruz ◽  
...  
Keyword(s):  
T Cell ◽  
In Vivo Imaging ◽  
Reporter Mouse ◽  
Cell Localization ◽  
Dual Color ◽  
And Function

scholarly journals TMOD-24. GENERATION AND CHARACTERIZATION OF A NOVEL TRANSGENIC IDO REPORTER MOUSE FOR IDO POSTTRANSLATIONAL MODIFICATION ANALYSIS IN SITU

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii233-ii233
Author(s):  
April Bell ◽  
Lijie Zhai ◽  
Erik Ladomersky ◽  
Kristen Lauing ◽  
Lakshmi Bollu ◽  
...  
Keyword(s):  
Mouse Model ◽  
Tumor Cell ◽  
Central Nervous System Tumor ◽  
Post Translational Modifications ◽  
Reporter Mouse ◽  
C Terminus ◽  
Human Gbm

Abstract Glioblastoma (GBM) is the most common and aggressive primary central nervous system tumor in adults with a median survival of 14.6 months. GBM is a potently immunosuppressive cancer due in-part to the prolific expression of immunosuppressive indoleamine 2,3 dioxygenase 1 (IDO). Tumor cell IDO facilitates the intratumoral accumulation of regulatory T cells (Tregs; CD4+CD25+FoxP3+). Although immunosuppressive IDO activity is canonically characterized by the conversion of tryptophan into kynurenine, we have utilized transgenic and syngeneic mouse models and mutant glioma lines to demonstrate that tumor cell IDO increases Treg accumulation independent of tryptophan metabolism. Here, we address the gap in our understanding of IDO signaling activity in vivo. Subcutaneously-engrafted human GBM expressing human IDO-GFP cDNA was isolated from immunodeficient humanized NSG-SGM3 mice. The tumor was immunoprecipitated for the GFP tag using GFP-TRAP followed by mass spectrometry which revealed a novel methylation site on a lysine residue at amino acid 373 in the IDO C-terminus region. Western blot analysis of IDO protein also revealed the presence of tyrosine phosphorylation. Additionally, we recently created a new transgenic IDO reporter mouse model whereby endogenous IDO is fused to GFP via a T2A linker (IDO→GFP). This model allows for the isolation of IDO+ cells in real-time and without causing cell death, thereby creating the opportunity for downstream molecular analysis of in situ-isolated GFP+ cells. Collectively, our work suggests that IDO non-enzyme activity may involve the post-translational modifications we recently identified. As IDO activity may differ between in vitro and in vivo modeling systems, we will use the new IDO→GFP reporter mouse model for an improved mechanistic understanding of how immunosuppressive IDO facilitates Treg accumulation in vivo.

scholarly journals A Fluorescent Reporter Mouse for Inflammasome Assembly Demonstrates an Important Role for Cell-Bound and Free ASC Specks during In Vivo Infection

Cell Reports ◽  
2016 ◽  
Vol 16 (2) ◽  
pp. 571-582 ◽  
Author(s):  
Te-Chen Tzeng ◽  
Stefan Schattgen ◽  
Brian Monks ◽  
Donghai Wang ◽  
Anna Cerny ◽  
...  
Keyword(s):  
Fluorescent Reporter ◽  
Reporter Mouse

scholarly journals Fryl deficiency is associated with defective kidney development and function in mice

2018 ◽  
Vol 243 (5) ◽  
pp. 408-417 ◽  
Author(s):  
Yong-Sub Byun ◽  
Eun-Kyoung Kim ◽  
Kimi Araki ◽  
Ken-ichi Yamamura ◽  
Kihoon Lee ◽  
...  
Keyword(s):  
Body Weight ◽  
Growth Retardation ◽  
Normal Development ◽  
Full Term ◽  
Lower Body ◽  
Mouse Line ◽  
Lower Body Weight ◽  
Gene Functions ◽  
Convoluted Tubules

FRY like transcription coactivator ( Fryl) gene located on chromosome 5 is a paralog of FRY microtubule binding protein ( Fry) in vertebrates. It encodes a protein with unknown functions. Fryl gene is conserved in various species ranging from eukaryotes to human. Although there are several reports on functions of Fry gene, functions of Fryl gene remain unclear. A mouse line containing null mutation in Fryl gene by gene trapping was produced in this study for the first time. The survival and growth of Fryl−/− mice were observed. Fryl gene expression levels in mouse tissues were determined and histopathologic analyses were conducted. Most Fryl−/− mice died soon after birth. Rare Fryl−/− survivors showed growth retardation with significantly lower body weight compared to their littermate controls. Although they could breed, more than half of Fryl−/− survivors died of hydronephrosis before age 1. No abnormal histopathologic lesion was apparent in full-term embryo or adult tissues except the kidney. Abnormal lining cell layer detachments from walls of collecting and convoluted tubules in kidneys were apparent in Fryl−/− neonates and full-term embryos. Fryl gene was expressed in renal tubular tissues including the glomeruli and convoluted and collecting tubules. This indicates that defects in tubular systems are associated with Fryl functions and death of Fryl−/− neonates. Fryl protein is required for normal development and functional maintenance of kidney in mice. This is the first report of in vivo Fryl gene functions. Impact statement FRY like transcription coactivator ( Fryl) gene is conserved in various species ranging from eukaryotes to human. It expresses a protein with unknown function. We generated a Fryl gene mutant mouse line and found that most homozygous mice died soon after their birth. Rare Fryl−/− survivors showed growth retardation with significantly lower body weight compared to their littermate controls. Although they could breed, more than half of Fryl−/− survivors died of hydronephrosis before age 1. Full-term mutant embryos showed abnormal collecting and convoluted tubules in kidneys where Fryl gene was expressed. Collectively, these results indicate that Fryl protein is required for normal development and functional maintenance of kidney in mice. To the best of our knowledge, this is the first report on in vivo Fryl gene functions.

scholarly journals Nas transgenic mouse line allows visualization of Notch pathway activity in vivo

genesis ◽  
2006 ◽  
Vol 44 (6) ◽  
pp. 277-286 ◽  
Author(s):  
Céline Souilhol ◽  
Sarah Cormier ◽  
Marie Monet ◽  
Sandrine Vandormael-Pournin ◽  
Anne Joutel ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Notch Pathway ◽  
Mouse Line ◽  
Pathway Activity ◽  
Transgenic Mouse Line

scholarly journals INTEGRINS MEDIATE PLACENTAL EXTRACELLULAR VESICLE TRAFFICKING TO LUNG AND LIVER IN VIVO

2020 ◽  
Author(s):  
Sean L. Nguyen ◽  
Soo Hyun Ahn ◽  
Jacob W. Greenberg ◽  
Benjamin W. Collaer ◽  
Dalen W. Agnew ◽  
...  
Keyword(s):  
Immune Cells ◽  
Extracellular Vesicle ◽  
Dependent Manner ◽  
Cellular Phenotype ◽  
Reporter Mouse ◽  
Membrane Bound ◽  
First Time

ABSTRACTMembrane-bound extracellular vesicles (EVs) mediate intercellular communication in all organisms, and those produced by placental mammals have become increasingly recognized as significant mediators of fetal-maternal communication. Here, we aimed to identify maternal cells targeted by placental EVs and elucidate the mechanisms by which they traffic to these cells. Exogenously administered pregnancy-associated EVs traffic specifically to the lung; further, placental EVs associate with lung interstitial macrophages and liver Kupffer cells in an integrin-dependent manner. Localization of EV to maternal lungs was confirmed in unmanipulated pregnancy using a transgenic reporter mouse model, which also provided in situ and in vitro evidence that fetally-derived EVs, rarely, may cause genetic alteration of maternal cells. These results provide for the first time direct in vivo evidence for targeting of placental EVs to maternal immune cells, and further, evidence that EVs can alter cellular phenotype.

scholarly journals Non-Invasive Quantification of Cartilage Using a Novel In Vivo Bioluminescent Reporter Mouse

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0130564 ◽  
Author(s):  
Sarah E. Mailhiot ◽  
Donald L. Zignego ◽  
Justin R. Prigge ◽  
Ella R. Wardwell ◽  
Edward E. Schmidt ◽  
...  
Keyword(s):  
Reporter Mouse ◽  
Non Invasive

scholarly journals The VEGF-A exon 8 splicing-sensitive fluorescent reporter mouse is a novel tool to assess the effects of splicing regulatory compounds in vivo

RNA Biology ◽  
2019 ◽  
Vol 16 (12) ◽  
pp. 1672-1681
Author(s):  
M. Stevens ◽  
E. Star ◽  
M. Lee ◽  
E. Innes ◽  
L. Li ◽  
...  
Keyword(s):  
Fluorescent Reporter ◽  
Reporter Mouse
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