scholarly journals The mito::mKate2 mouse: A far-red fluorescent reporter mouse line for tracking mitochondrial dynamics in vivo

genesis ◽  
2017 ◽  
Vol 56 (2) ◽  
pp. e23087 ◽  
Author(s):  
Anthony P. Barrasso ◽  
Xuefei Tong ◽  
Ross A. Poché
Keyword(s):  
Mitochondrial Dynamics ◽  
Mouse Line ◽  
Fluorescent Reporter ◽  
Reporter Mouse

scholarly journals A Fluorescent Reporter Mouse for Inflammasome Assembly Demonstrates an Important Role for Cell-Bound and Free ASC Specks during In Vivo Infection

Cell Reports ◽  
2016 ◽  
Vol 16 (2) ◽  
pp. 571-582 ◽  
Author(s):  
Te-Chen Tzeng ◽  
Stefan Schattgen ◽  
Brian Monks ◽  
Donghai Wang ◽  
Anna Cerny ◽  
...  
Keyword(s):  
Fluorescent Reporter ◽  
Reporter Mouse

scholarly journals The VEGF-A exon 8 splicing-sensitive fluorescent reporter mouse is a novel tool to assess the effects of splicing regulatory compounds in vivo

RNA Biology ◽  
2019 ◽  
Vol 16 (12) ◽  
pp. 1672-1681
Author(s):  
M. Stevens ◽  
E. Star ◽  
M. Lee ◽  
E. Innes ◽  
L. Li ◽  
...  
Keyword(s):  
Fluorescent Reporter ◽  
Reporter Mouse

scholarly journals Live imaging YAP signaling in vivo with fusion reporter mice

2021 ◽  
Author(s):  
Bin Gu ◽  
Brian Bradshaw ◽  
Min Zhu ◽  
Yu Sun ◽  
Sevan Hopyan ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Cell Polarity ◽  
Near Infrared ◽  
Kinase Activity ◽  
Live Imaging ◽  
Mouse Line ◽  
Reporter Mouse ◽  
Reporter Mice ◽  
Mammalian Embryonic Development

YAP protein is a critical regulator of mammalian embryonic development. By generating a near-infrared fusion YAP reporter mouse line, we have achieved high-resolution live imaging of YAP localization during mouse embryonic development. We have validated the reporter by demonstrating its predicted responses to blocking Lats kinase activity or blocking cell polarity. The YAP fusion reporter mice and imaging methods will open new opportunities for understanding dynamic YAP signaling in vivo in many different situations.

scholarly journals Generation of a dual-color reporter mouse line to monitor spermatogenesis in vivo

2014 ◽  
Vol 2 ◽  
Author(s):  
Yoshinori Makino ◽  
Erina Inoue ◽  
Masashi Hada ◽  
Keisuke Aoshima ◽  
Satsuki Kitano ◽  
...  
Keyword(s):  
Mouse Line ◽  
Reporter Mouse ◽  
Dual Color

scholarly journals Skeletal muscle tissue secretes more extracellular vesicles than white adipose tissue and myofibers are a major source ex vivo but not in vivo

2020 ◽  
Author(s):  
Andrea L. Estrada ◽  
Zackary Valenti ◽  
Gabriella Hehn ◽  
Christopher P. Allen ◽  
Nicole A. Kruh-Garcia ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Extracellular Vesicles ◽  
White Adipose Tissue ◽  
Ex Vivo ◽  
Fluorescent Reporter ◽  
Reporter Mouse ◽  
Relative Contribution ◽  
Human Protein Atlas

AbstractCirculating extracellular vesicles (EVs) are biomarkers of and contributors to the etiology of disease. Skeletal muscle (SkM) and white adipose tissue (WAT) are the two largest organs by mass in humans and rodents but the relative contribution of EVs from these tissues is unknown. We hypothesized that SkM tissue secretes more EVs than WAT and that a dual fluorescent reporter mouse could be used to detect SkM myofiber-derived EVs in vivo. Human Protein Atlas data and directly measuring EV secretion in mouse SkM and WAT using an ex vivo tissue explant model confirmed that SkM tissue secretes more EVs than WAT. Differences in EV secretion between SkM and WAT were not due to SkM contraction but may be explained by differences in tissue metabolic capacity. A SkM myofiber-specific dual fluorescent reporter mouse was created. Spectral flow cytometry revealed that SkM myofibers are a major source of SkM tissue-derived EVs ex vivo but few reach the circulation in vivo. Our findings demonstrate that SkM secretes more EVs than WAT and many come from SkM myofibers, but our in vivo data indicate that EVs secreted by SkM myofibers may remain primarily in their local extracellular environment.

scholarly journals Mitochondrial arginase-2 is essential for IL-10 metabolic reprogramming of inflammatory macrophages

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jennifer K. Dowling ◽  
Remsha Afzal ◽  
Linden J. Gearing ◽  
Mariana P. Cervantes-Silva ◽  
Stephanie Annett ◽  
...  
Keyword(s):  
Metabolic Regulation ◽  
Mitochondrial Dynamics ◽  
Interleukin 10 ◽  
Metabolic Reprogramming ◽  
In Vivo Model ◽  
Macrophage Polarisation ◽  
Inflammatory Status ◽  
Inflammatory Macrophages

AbstractMitochondria are important regulators of macrophage polarisation. Here, we show that arginase-2 (Arg2) is a microRNA-155 (miR-155) and interleukin-10 (IL-10) regulated protein localized at the mitochondria in inflammatory macrophages, and is critical for IL-10-induced modulation of mitochondrial dynamics and oxidative respiration. Mechanistically, the catalytic activity and presence of Arg2 at the mitochondria is crucial for oxidative phosphorylation. We further show that Arg2 mediates this process by increasing the activity of complex II (succinate dehydrogenase). Moreover, Arg2 is essential for IL-10-mediated downregulation of the inflammatory mediators succinate, hypoxia inducible factor 1α (HIF-1α) and IL-1β in vitro. Accordingly, HIF-1α and IL-1β are highly expressed in an LPS-induced in vivo model of acute inflammation using Arg2−/− mice. These findings shed light on a new arm of IL-10-mediated metabolic regulation, working to resolve the inflammatory status of the cell.

scholarly journals Machine learning-based classification of mitochondrial morphology in primary neurons and brain

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Garrett M. Fogo ◽  
Anthony R. Anzell ◽  
Kathleen J. Maheras ◽  
Sarita Raghunayakula ◽  
Joseph M. Wider ◽  
...  
Keyword(s):  
Image Analysis ◽  
Cortical Neurons ◽  
Mitochondrial Dynamics ◽  
Automated Image Analysis ◽  
Mitochondrial Morphology ◽  
Analysis Pipeline ◽  
Genetic Ablation ◽  
Block Face

AbstractThe mitochondrial network continually undergoes events of fission and fusion. Under physiologic conditions, the network is in equilibrium and is characterized by the presence of both elongated and punctate mitochondria. However, this balanced, homeostatic mitochondrial profile can change morphologic distribution in response to various stressors. Therefore, it is imperative to develop a method that robustly measures mitochondrial morphology with high accuracy. Here, we developed a semi-automated image analysis pipeline for the quantitation of mitochondrial morphology for both in vitro and in vivo applications. The image analysis pipeline was generated and validated utilizing images of primary cortical neurons from transgenic mice, allowing genetic ablation of key components of mitochondrial dynamics. This analysis pipeline was further extended to evaluate mitochondrial morphology in vivo through immunolabeling of brain sections as well as serial block-face scanning electron microscopy. These data demonstrate a highly specific and sensitive method that accurately classifies distinct physiological and pathological mitochondrial morphologies. Furthermore, this workflow employs the use of readily available, free open-source software designed for high throughput image processing, segmentation, and analysis that is customizable to various biological models.

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