scholarly journals Stinging nettle and neem enhance antibody response to local killed and imported live infectious bursal disease vaccines in indigenous chicken in Kenya

2018 ◽  
Vol 97 (2) ◽  
pp. 447-454 ◽  
Author(s):  
M.O. Bwana ◽  
L.W. Njagi ◽  
P.N. Nyaga ◽  
P.G. Mbuthia ◽  
L.C. Bebora ◽  
...  
Keyword(s):  
Antibody Response ◽  
Infectious Bursal Disease ◽  
Stinging Nettle ◽  
Indigenous Chicken ◽  
Bursal Disease ◽  
Enhance Antibody Response

scholarly journals Ascorbic acid supplementation improved antibody response to infectious bursal disease vaccination in chickens

2000 ◽  
Vol 79 (5) ◽  
pp. 680-688 ◽  
Author(s):  
J. Amakye-Anim ◽  
T.L. Lin ◽  
P.Y. Hester ◽  
D. Thiagarajan ◽  
B.A. Watkins ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Antibody Response ◽  
Infectious Bursal Disease ◽  
Acid Supplementation ◽  
Bursal Disease ◽  
Ascorbic Acid Supplementation

Infectious Bursal Disease Viral Infections. I. Complement and Virus-Neutralizing Antibody Response Following Infection of Susceptible Chickens

1979 ◽  
Vol 23 (1) ◽  
pp. 95 ◽  
Author(s):  
J. K. Skeeles ◽  
P. D. Lukert ◽  
E. V. De Buysscher ◽  
O. J. Fletcher ◽  
J. Brown
Keyword(s):  
Antibody Response ◽  
Viral Infections ◽  
Neutralizing Antibody ◽  
Infectious Bursal Disease ◽  
Bursal Disease

scholarly journals Comparison of two attenuated infectious bursal disease vaccine strains focused on safety and antibody response in commercial broilers

2021 ◽  
Vol 14 (1) ◽  
pp. 70-77
Author(s):  
Thotsapol Thomrongsuwannakij ◽  
Nataya Charoenvisal ◽  
Niwat Chansiripornchai
Keyword(s):  
Phylogenetic Analysis ◽  
Antibody Response ◽  
Vaccine Strain ◽  
Broiler Chickens ◽  
Humoral Response ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Strain Group ◽  
Vaccine Responses ◽  
Bursal Disease

Background and Aim: Infectious bursal disease (IBD) or Gumboro disease is one of the most detrimental diseases in the poultry industry worldwide. Previous scientific studies have shown that live IBD vaccination might induce transient immunosuppression, leading to suboptimal vaccine responses, and therefore lack of protection against other infectious diseases; therefore, selecting an IBD vaccine in commercial farms is a concern. This study aims to compare two commercially attenuated IBD vaccines (intermediate and intermediate-plus strains) in terms of safety and antibody response to IBD and Newcastle disease viruses (NDV) in commercial broilers. Materials and Methods: Overall, 216 Cobb broiler chickens were divided into three groups based on the IBD vaccine strain administered: V217 strain (Group 1), M.B. strain (Group 2), and an unvaccinated group (Group 3). Groups 1 and 2 were orally vaccinated with Hitchner B1 NDV vaccine strain 7 days after IBD vaccination. Blood samples were collected at IBD vaccination day (15 days of age) and at 7, 14, 21, and 28 days post-IBD vaccination. The immunosuppressive effects of the IBD vaccination were determined by NDV antibody response, the bursa:body weight (B:BW) ratio, and the histopathological lesion scores of the bursa of Fabricius. Phylogenetic analysis was also performed. Results: Phylogenetic analysis revealed that the M.B. strain belonged to a very virulent IBD strain, whereas the V217 strain belonged to a classical IBD virus strain. NDV antibody titers of the two vaccinated groups increased after ND vaccination, reaching their maximum at 14 days post-ND vaccination and decreasing thereafter. The V217 group presented the highest NDV humoral response from 7 days post-vaccination (dpv) to the end of the study. The mean NDV antibody titer of the V217 group was significantly (p<0.05) higher than that of the M.B. group at 14 dpv. In addition, the V217 strain-induced lower bursal lesions post-IBD vaccination and a higher B:BW ratio at 7 and 21 dpv compared to the M.B. group. The higher B:BW ratio, lower bursal lesions, and higher ND antibody response present in the V217 group indicate that the V217 strain induces lower immunosuppressive effects compared to the M.B. strain. Conclusion: The results of this study indicate that IBD vaccine selection merits consideration, as avoiding the immunosuppressive effects induced by live IBD vaccination and the consequent impact on response to other vaccines is important.

scholarly journals Decay pattern of maternally derived antibodies and antibody response of infectious bursal diseases live vaccine

2019 ◽  
pp. 18-23
Keyword(s):  
Antibody Response ◽  
Live Vaccine ◽  
Blood Sampling ◽  
Infectious Bursal Disease ◽  
Broiler Chicks ◽  
Study Methods ◽  
Bursal Disease ◽  
Decay Pattern ◽  
The Right ◽  
Elisa Titre

Introduction: Repeated outbreaks of infectious bursal disease (IBD) despite vaccination necessitated this study. Methods: The right age of IBD vaccination, the immunogenicity and the strain of the vaccine were established. Boven gold pullets (n = 150) were used. They were grouped into 6 groups; unvaccinated and vaccinated chicks according to weeks of blood sampling. Both antibodies titres (unvaccinated and vaccinated) were determined by enzyme- linked immune sorbent assay (ELISA). Results: In broiler chicks, the maternally-derived antibodies (MDA) titre decayed from 1,500 ELISA titre at day old to zero at 35 days , while the antibody rose from a titre of 500 at day old to titre of 2000 at 40 days following vaccination and intercepted at a titre of 700 at 21 days. In pullet chicks, the MDA started with an ELISA titre of 1,500 at day old to zero at 38 days of age, while antibodies rose from a titre of 1,100 at day old to 2,000 at 35 days, and then intercepted at a titre of 800 at 22 days of age, hence the breakthrough titre of the live vaccine for pullet chicks. Significance: MDA for broiler and pullet chicks for the chicks from this hatchery completely decayed at 35 and 38 days, respectively and intercepted at a titre of 700. Thus, the chicks should be vaccinated at 21 and 22 days, respectively. Also, poultry farmer should not use the same vaccination days for chicks of different hatchery. The breakthrough titre of the vaccine was 700. Therefore, it is an intermediate plus. The decay was faster in broilers than pullets, hence vaccination days should be early in broilers than pullets.

scholarly journals An unusual outbreak of inclusion body hepatitis on a broiler chicken farm: a case report

2017 ◽  
Vol 62 (No. 11) ◽  
pp. 631-635
Author(s):  
V. Revajova ◽  
R. Herich ◽  
V. Seman ◽  
M. Levkut Jr ◽  
M. Levkutova ◽  
...  
Keyword(s):  
Antibody Response ◽  
Disease Virus ◽  
Inclusion Body ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Antibody Detection ◽  
Chicken Anaemia Virus ◽  
Fowl Adenovirus ◽  
Inclusion Body Hepatitis ◽  
Bursal Disease

This study investigated an outbreak of inclusion body hepatitis in ROSS 308 hybrid broiler type chickens between 19 and 25 days of fattening. For this purpose, clinical observation, ELISA fowl adenovirus and chicken anaemia virus antibody detection in serum at 21 and 42 days, mortality evaluation, epidemiological analysis, histology and genetic identification were performed. The six flocks of the farm consisted of 90,000 chickens. Only one flock of 15,000 chickens was affected on this farm. At 19 days of age, ill chickens showed clinical signs of depression, anorexia, somnolence, ruffled feathers, anaemic comb and wattles and occasionally nervous signs. Based on ELISA titres, the antibody response to fowl adenovirus increased greatly from 21 to 42 days. The antibody response to vaccination against infectious bursal disease virus and chicken anaemia virus was at the expected level in all broiler flocks. Necropsy showed diffuse petechial and ecchymotic haemorrhages in skeletal muscles, liver, pancreas, kidney, together with hepatomegaly, splenomegaly and catarrhal enteritis. Histologically, fatty liver degeneration, multifocal liver necrosis and intranuclear inclusions in hepatocytes, as well as focal necrosis in pancreas and spleen parenchyma were seen. The DNA of AAV-1 (avian adenovirus group 1) was detected using the PCR method in paraffin-embedded liver samples. The results revealed no association of inclusion body hepatitis with infectious bursal disease virus or chicken anaemia virus infection, and suggested primary disease. However, the involvement of only one chicken flock on the farm remains unexplained.

scholarly journals Isolation of Infectious Bursal Disease Virus Using Indigenous Chicken Embryos in Kenya

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
W. U. Mutinda ◽  
L. W. Njagi ◽  
P. N. Nyaga ◽  
L. C. Bebora ◽  
P. G. Mbuthia ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Economic Activities ◽  
Indigenous Chicken ◽  
Chicken Embryos ◽  
Local Resources ◽  
Bursal Disease ◽  
Specific Pathogen

Infectious bursal disease virus (IBDV) isolates were recovered from outbreaks to initiate activities towards developing a local vaccine strain. Use of indigenous chicken embryos was exploited to determine their potential, promote utilization of local resources for research, and enhance household economic activities. Bursa of Fabricius (BFs) samples from outbreaks shown to be IBDV positive was homogenized and inoculated in 4-week-old specific pathogen-free (SPF) IBDV seronegative white leghorn chicks. The harvested virus was inoculated into 11-day-old indigenous chicken embryos that were IBDV seronegative and passaged serially three times after which they were inoculated into 4-week-old indigenous chicks to test for presence and virulence of propagated virus. Out of 153 BFs collected from outbreaks, 43.8% (67/153) were positive for IBDV antigen and 65.7% (44/67) caused disease in SPF chicks. The embryo mean mortalities were 88% on primary inoculation, 94% in 1st passage, 91% in 2nd passage, and 67% in 3rd passage. After the third passage in embryos all the 44 isolates were virulent in 4-week-old indigenous chicks. The results show that indigenous chicken embryos support growth of IBDV and can be used to propagate the virus as an alternative viral propagating tool for respective vaccine preparation.

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