scholarly journals Comparison of goblet cell staining methods in jejunal mucosa of turkey poults

2017 ◽  
Vol 96 (3) ◽  
pp. 556-559 ◽  
Author(s):  
S.O. Osho ◽  
T. Wang ◽  
N.L. Horn ◽  
O. Adeola
Keyword(s):  
Goblet Cell ◽  
Jejunal Mucosa ◽  
Cell Staining ◽  
Turkey Poults ◽  
Staining Methods

0439 Comparison of intestinal goblet cell staining methods in turkey poults

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 211-211
Author(s):  
S. O. Osho ◽  
T. Wang ◽  
N. L. Horn ◽  
O. Adeola
Keyword(s):  
Goblet Cell ◽  
Cell Staining ◽  
Turkey Poults ◽  
Staining Methods

scholarly journals Fluorescent Cell Staining Methods for Living Hypsibius exemplaris Embryos

2018 ◽  
Vol 2018 (11) ◽  
pp. pdb.prot106021 ◽  
Author(s):  
Kristen M. McGreevy ◽  
Kira L. Heikes ◽  
Shiri Kult ◽  
Marla E. Tharp ◽  
Bob Goldstein
Keyword(s):  
Fluorescent Cell ◽  
Cell Staining ◽  
Staining Methods

scholarly journals Multiparameter Characterization Confirms Apoptosis as the Primary Cause of Reduced Self-renewal Capacity in Cultured Human Fetal Neural Stem Cells

2016 ◽  
Vol 38 (6) ◽  
pp. 2123-2138 ◽  
Author(s):  
Yunqian Guan ◽  
Xiaobo Li ◽  
Haiqiang Zou ◽  
Xiaoming Yan ◽  
Chunsong Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Annexin V ◽  
Live Cell ◽  
Self Renewal ◽  
Cell Staining ◽  
Staining Methods ◽  
Apoptosis Process ◽  
Multiparameter Analysis ◽  
Fixed Cell

Background: Human fetal striatum-derived neural stem cells (hfsNSCs) are important in regenerative medicine; however, their ability to self-renew diminishes quickly following passages in culture. Typically when hfsNSC-derived neurospheres are dissociated by accutase, more than 90% of the cells survive, but only 6-8% of the cells are able to form secondary neurospheres. Our hypothesis is that the hfsNSCs that are unable to form new neurospheres become apoptotic. Methods/Results: Because the NSC apoptosis process has never been characterized in detail, we characterized hfsNSC apoptosis using multiparameter analysis and determined that the majority of hfsNSCs undergo apoptosis after passaging, which leads to a reduction in self-renewal. The replacement of trituration with vortexing decreases apoptosis, increases self-renewal, and does not affect NSC differentiation. When we used live cell staining with Annexin V, Hoechst 33342, and PI together, the apoptotic index was in agreement with what could be obtained using fixed-cell staining methods, including TUNEL and activated caspase-3 immunocytochemistry. NSC apoptosis could be divided into 9 stage types based on our live cell assay. Several types during early and late stages had similar staining profiles that could be further discriminated based on cell size. Conclusion: Apoptosis largely contributes to the low self-renewal of neurospheres, and replacing trituration with vortexing aided in alleviating NSC apoptosis. Multiparameter analysis is required for the identification of NSC apoptosis, particularly when live cell staining is used.

scholarly journals Replacing dietary antibiotics with 0.20% l-glutamine and synbiotics following weaning and transport in pigs

2020 ◽  
Vol 98 (9) ◽  
Author(s):  
Betty R McConn ◽  
Alan W Duttlinger ◽  
Kouassi R Kpodo ◽  
Susan D Eicher ◽  
Brian T Richert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Performance ◽  
Cell Count ◽  
Goblet Cell ◽  
Negative Control ◽  
Jejunal Mucosa ◽  
Intestinal Health ◽  
Necrosis Factor Alpha ◽  
Study Objective ◽  
Dietary Treatments

Abstract Dietary antibiotic use has been limited in swine production due to concerns regarding antibiotic resistance. However, this may negatively impact the health, productivity, and welfare of pigs. Therefore, the study objective was to determine if combining dietary synbiotics and 0.20% l-glutamine would improve pig growth performance and intestinal health following weaning and transport when compared with traditionally used dietary antibiotics. Because previous research indicates that l-glutamine improves swine growth performance and synbiotics reduce enterogenic bacteria, it was hypothesized that supplementing diets with 0.20% l-glutamine (GLN) and synbiotics (SYN; 3 strains of Lactobacillus [1.2 × 10^9 cfu/g of strain/pig/d] + β-glucan [0.01 g/pig/d] + fructooligosaccharide [0.01 g/pig/d]) would have an additive effect and improve pig performance and intestinal health over that of dietary antibiotics. Mixed-sex pigs (N = 226; 5.86 ± 0.11 kg body weight [BW]) were weaned (19.4 ± 0.2 d of age) and transported for 12 h in central Indiana. Pigs were blocked by BW and allotted to one of two dietary treatments (5 to 6 pigs per pen): antibiotics (positive control [PC]; chlortetracycline [441 ppm] + tiamulin [38.5 ppm]), no antibiotics (negative control [NC]), GLN, SYN, or the NC diet with both the GLN and SYN additives (GLN + SYN) fed for 14 d. From day 14 post-weaning to the end of the grow-finish period, all pigs were provided common antibiotic-free diets. Data were analyzed using PROC GLIMMIX and PROC MIXED in SAS 9.4. Overall, haptoglobin was greater (P = 0.03; 216%) in NC pigs compared with PC pigs. On day 13, GLN and PC pigs tended to have reduced (P = 0.07; 75.2% and 67.3%, respectively) haptoglobin compared with NC pigs. On day 34, the jejunal goblet cell count per villi and per millimeter tended to be greater (P < 0.08; 71.4% and 62.9%, respectively) in SYN pigs compared with all other dietary treatments. Overall, jejunal mucosa tumor necrosis factor-alpha (TNFα) gene expression tended to be greater (P = 0.09; 40.0%) in NC pigs compared with PC pigs on day 34. On day 34, jejunal mucosa TNFα gene expression tended to be greater (P = 0.09; 33.3%, 41.2%, and 60.0%, respectively) in GLN pigs compared with SYN, GLN + SYN, and PC pigs. Although it was determined that some metrics of pig health were improved by the addition of GLN and SYN (i.e., haptoglobin and goblet cell count), overall, there were very few differences detected between dietary treatments and this may be related to the stress load incurred by the pigs.

THE CHANGES WHICH OCCUR WITH AGEING IN THE VASCULAR PATTERN OF THE INFUNDIBULAR PROCESS OF THE HUMAN HYPOPHYSIS CEREBRI

1954 ◽  
Vol 10 (3) ◽  
pp. 238-NP ◽  
Author(s):  
G. P. XUEREB
Keyword(s):  
Blood Vessels ◽  
Red Cell ◽  
Vascular Pattern ◽  
Simple Pattern ◽  
Neural Lobe ◽  
Human Pituitary ◽  
Cell Staining ◽  
Simple Arrangement ◽  
Staining Methods ◽  
The Individual

SUMMARY A study of the smaller blood vessels has been carried out on 170 human pituitary bodies by means of red cell staining methods. It was found that the vascular pattern of the neural lobe undergoes alteration with advancing years, the changes being common to both the sexes. In infancy and in childhood, a simple pattern of branching vessels is seen throughout the neural lobe. In the adolescent, a change from one type of vascular pattern to another becomes apparent: first, the individual vessels become tortuous and, subsequently, the tortuous vessels tend to become arranged in groups. In the adult, the pattern becomes increasingly complex, and tortuous vessels of large calibre are seen grouped in lobular units. In old age, the degree of vascularity of the tissue is much reduced and the pattern of the blood vessels tends to revert gradually to the simple arrangement seen in childhood.

Pathogenesis of Avian Pneumovirus Infection in Turkeys

2002 ◽  
Vol 39 (3) ◽  
pp. 300-310 ◽  
Author(s):  
F. F. Jirjis ◽  
S. L. Noll ◽  
D. A. Halvorson ◽  
K. V. Nagaraja ◽  
D. P. Shaw
Keyword(s):  
Clinical Signs ◽  
Nasal Discharge ◽  
Serum Samples ◽  
Avian Pneumovirus ◽  
Bordetella Avium ◽  
Turkey Poults ◽  
Staining Methods ◽  
Tracheal Tissue ◽  
Inflammatory Changes ◽  
Ocular Discharge

Avian pneumovirus (APV) is the cause of a respiratory disease of turkeys characterized by coughing, ocular and nasal discharge, and swelling of the infraorbital sinuses. Sixty turkey poults were reared in isolation conditions. At 3 weeks of age, serum samples were collected and determined to be free of antibodies against APV, avian influenza, hemorrhagic enteritis, Newcastle disease, Mycoplasma gallisepticum, Mycoplasma synoviae, Mycoplasma meleagridis, Ornithobacterium rhinotracheale, and Bordetella avium. When the poults were 4 weeks old, they were inoculated with cell culture–propagated APV (APV/Minnesota/turkey/2a/97) via the conjunctival spaces and nostrils. After inoculation, four poults were euthanatized every 2 days for 14 days, and blood, swabs, and tissues were collected. Clinical signs consisting of nasal discharge, swelling of the infraorbital sinuses, and frothy ocular discharge were evident by 2 days postinoculation (PI) and persisted until day 12 PI. Mild inflammation of the mucosa of the nasal turbinates and infraorbital sinuses was present between days 2 and 10 PI. Mild inflammatory changes were seen in tracheas of poults euthanatized between days 4 and 10 PI. Antibody to APV was detected by day 7 PI. The virus was detected in tissue preparations and swabs of nasal turbinates and infraorbital sinuses by reverse transcription polymerase chain reaction, virus isolation, and immunohistochemical staining methods between days 2 and 10 PI. Virus was detected in tracheal tissue and swabs between days 2 and 6 PI using the same methods. In this experiment, turkey poults inoculated with tissue culture-propagated APV developed clinical signs similar to those seen in field cases associated with infection with this virus.

Uranyl Acetate and Rotary Shadowing to Increase the Contrast of Small Aggregated Virus Particles

1969 ◽  
Vol 27 ◽  
pp. 424-425
Author(s):  
Lee F. Ellis ◽  
Richard M. Van Frank ◽  
Walter J. Kleinschmidt
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Density Gradient ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Uranyl Acetate ◽  
Interferon Inducer ◽  
Virus Particles ◽  
Staining Methods ◽  
Rotary Shadowing

The extract from Penicillum stoliniferum, known as statolon, has been purified by density gradient centrifugation. These centrifuge fractions contained virus particles that are an interferon inducer in mice or in tissue culture. Highly purified preparations of these particles are difficult to enumerate by electron microscopy because of aggregation. Therefore a study of staining methods was undertaken.

Advances in imaging SIMS studies of stained and BrdU-labelled human metaphase chromosomes

1995 ◽  
Vol 53 ◽  
pp. 932-933
Author(s):  
R. Levi-Setti ◽  
J. M. Chabala ◽  
R. Espinosa ◽  
M. M. Le Beau
Keyword(s):  
High Performance ◽  
Secondary Ion Mass Spectrometry ◽  
Analytical Sensitivity ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Sector Mass Spectrometer ◽  
Staining Methods ◽  
The University ◽  
Secondary Ion ◽  
Better Than

We have shown previously that isotope-labelled nucleotides in human metaphase chromosomes can be detected and mapped by imaging secondary ion mass spectrometry (SIMS), using the University of Chicago high resolution scanning ion microprobe (UC SIM). These early studies, conducted with BrdU- and 14C-thymidine-labelled chromosomes via detection of the Br and 28CN- (14C14N-> labelcarrying signals, provided some evidence for the condensation of the label into banding patterns along the chromatids (SIMS bands) reminiscent of the well known Q- and G-bands obtained by conventional staining methods for optical microscopy. The potential of this technique has been greatly enhanced by the recent upgrade of the UC SIM, now coupled to a high performance magnetic sector mass spectrometer in lieu of the previous RF quadrupole mass filter. The high transmission of the new spectrometer improves the SIMS analytical sensitivity of the microprobe better than a hundredfold, overcoming most of the previous imaging limitations resulting from low count statistics.

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