scholarly journals Full-length genome sequencing analysis of avian infectious bronchitis virus isolate associated with nephropathogenic infection

2016 ◽  
Vol 95 (12) ◽  
pp. 2921-2929 ◽  
Author(s):  
R.A. Leghari ◽  
B. Fan ◽  
H. Wang ◽  
J. Bai ◽  
L. Zhang ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Infectious Bronchitis Virus ◽  
Virus Isolate ◽  
Full Length ◽  
Sequencing Analysis ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
Infectious Bronchitis Virus Isolate ◽  
Full Length Genome

scholarly journals Pathogenesis and Tissue Distribution of Avian Infectious Bronchitis Virus Isolate IRFIBV32 (793/B Serotype) in Experimentally Infected Broiler Chickens

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Zahra Boroomand ◽  
Keramat Asasi ◽  
Ali Mohammadi
Keyword(s):  
Tissue Distribution ◽  
Infectious Bronchitis Virus ◽  
Broiler Chickens ◽  
Virus Isolate ◽  
Clinical Signs ◽  
Viral Rna ◽  
Control Group ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
Infectious Bronchitis Virus Isolate

Infectious bronchitis (IB) is one of the most important viral diseases of poultry. The aim of this study was to investigate the distribution of avian infectious bronchitis virus isolate IRFIBV32 (793/B serotype) in experimentally infected chicken. Ninety-one-day-old commercial broilers were divided randomly into two groups (seventy in the experimental and twenty in the control group). Chicks in the experimental group were inoculated intranasally with 105ELD50/0.1 mL of the virus at three weeks of age. The samples from various tissues were collected at1, 2, 3, 5, 7, 11, 13, 15, and 20 days postinoculation. Chickens exhibited mild respiratory signs and depression. Viral RNA was detected in the kidney, lung and tracheas on days 1 to 13 PI, in the oviduct between, days 3 and 13, in testes between days 1 and 11 PI, and in the caecal tonsil consistently up to day 20 PI. The most remarkable clinical signs and virus detection appeared on day 1 PI. Data indicated that the number of infected chickens and viral RNA detection from tissues was reduced with increasing antibody titer on day 20 PI. The results demonstrated that the IRFIBV32 virus has wide tissue distribution for respiratory, urogenital, and digestive systems.

Characterization and analysis of the full-length genome of a strain of the European QX-like genotype of infectious bronchitis virus

2012 ◽  
Vol 157 (6) ◽  
pp. 1211-1215 ◽  
Author(s):  
Shahid Hussain Abro ◽  
Lena H. M. Renström ◽  
Karin Ullman ◽  
Sándor Belák ◽  
Claudia Baule
Keyword(s):  
Infectious Bronchitis Virus ◽  
Full Length ◽  
Infectious Bronchitis ◽  
Full Length Genome

Intestinal Tropism of an Infectious Bronchitis Virus Isolate Not Explained by Spike Protein Binding Specificity

2019 ◽  
Vol 64 (1) ◽  
pp. 23 ◽  
Author(s):  
Farjana Saiada ◽  
Rodrigo A. Gallardo ◽  
H. L. Shivaprasad ◽  
Charles Corsiglia ◽  
Vicky L. Van Santen
Keyword(s):  
Protein Binding ◽  
Infectious Bronchitis Virus ◽  
Virus Isolate ◽  
Binding Specificity ◽  
Spike Protein ◽  
Infectious Bronchitis ◽  
Infectious Bronchitis Virus Isolate

scholarly journals Full-length Characterization of S1 Gene of Iranian QX Avian Infectious Bronchitis Virus Isolates, 2015

2016 ◽  
Vol 10 (4) ◽  
pp. 18-25 ◽  
Author(s):  
Homayoon Davam ◽  
Arash Ghalyanchi langeroudi ◽  
Masoud Hashemzadeh ◽  
Vahid Karimi ◽  
Taha Zabihi ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Full Length ◽  
Avian Infectious Bronchitis Virus ◽  
S1 Gene ◽  
Infectious Bronchitis ◽  
Virus Isolates

scholarly journals Molecular characterization of an avian GA13-like infectious bronchitis virus full-length genome from Costa Rica

VirusDisease ◽  
2021 ◽  
Author(s):  
Ricardo A. Villalobos-Agüero ◽  
Lisbeth Ramírez-Carvajal ◽  
Rebeca Zamora-Sanabria ◽  
Bernal León ◽  
James Karkashian-Córdoba
Keyword(s):  
Costa Rica ◽  
Molecular Characterization ◽  
Infectious Bronchitis Virus ◽  
Full Length ◽  
Infectious Bronchitis ◽  
Full Length Genome

scholarly journals Multiple novel non-canonically transcribed sub-genomic mRNAs produced by avian coronavirus infectious bronchitis virus

2020 ◽  
Vol 101 (10) ◽  
pp. 1103-1118
Author(s):  
Sarah Keep ◽  
Michael S. Oade ◽  
Filip Lidzbarski-Silvestre ◽  
Kirsten Bentley ◽  
Phoebe Stevenson-Leggett ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Allantoic Fluid ◽  
Full Length ◽  
N Gene ◽  
Regulatory Sequences ◽  
Sequencing Analysis ◽  
Accessory Gene ◽  
Infectious Bronchitis ◽  
Discontinuous Transcription

Coronavirus sub-genomic mRNA (sgmRNA) synthesis occurs via a process of discontinuous transcription involving complementary transcription regulatory sequences (TRSs), one (TRS-L) encompassing the leader sequence of the 5′ untranslated region (UTR), and the other upstream of each structural and accessory gene (TRS-B). Several coronaviruses have an ORF located between the N gene and the 3′-UTR, an area previously thought to be non-coding in the Gammacoronavirus infectious bronchitis virus (IBV) due to a lack of a canonical TRS-B. Here, we identify a non-canonical TRS-B allowing for a novel sgmRNA relating to this ORF to be produced in several strains of IBV: Beaudette, CR88, H120, D1466, Italy-02 and QX. Interestingly, the potential protein produced by this ORF is prematurely truncated in the Beaudette strain. A single nucleotide deletion was made in the Beaudette strain allowing for the generation of a recombinant IBV (rIBV) that had the potential to express a full-length protein. Assessment of this rIBV in vitro demonstrated that restoration of the full-length potential protein had no effect on viral replication. Further assessment of the Beaudette-derived RNA identified a second non-canonically transcribed sgmRNA located within gene 2. Deep sequencing analysis of allantoic fluid from Beaudette-infected embryonated eggs confirmed the presence of both the newly identified non-canonically transcribed sgmRNAs and highlighted the potential for further yet unidentified sgmRNAs. This HiSeq data, alongside the confirmation of non-canonically transcribed sgmRNAs, indicates the potential of the coronavirus genome to encode a larger repertoire of genes than has currently been identified.

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