scholarly journals Pathogenesis and Tissue Distribution of Avian Infectious Bronchitis Virus Isolate IRFIBV32 (793/B Serotype) in Experimentally Infected Broiler Chickens

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Zahra Boroomand ◽  
Keramat Asasi ◽  
Ali Mohammadi
Keyword(s):  
Tissue Distribution ◽  
Infectious Bronchitis Virus ◽  
Broiler Chickens ◽  
Virus Isolate ◽  
Clinical Signs ◽  
Viral Rna ◽  
Control Group ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
Infectious Bronchitis Virus Isolate

Infectious bronchitis (IB) is one of the most important viral diseases of poultry. The aim of this study was to investigate the distribution of avian infectious bronchitis virus isolate IRFIBV32 (793/B serotype) in experimentally infected chicken. Ninety-one-day-old commercial broilers were divided randomly into two groups (seventy in the experimental and twenty in the control group). Chicks in the experimental group were inoculated intranasally with 105ELD50/0.1 mL of the virus at three weeks of age. The samples from various tissues were collected at1, 2, 3, 5, 7, 11, 13, 15, and 20 days postinoculation. Chickens exhibited mild respiratory signs and depression. Viral RNA was detected in the kidney, lung and tracheas on days 1 to 13 PI, in the oviduct between, days 3 and 13, in testes between days 1 and 11 PI, and in the caecal tonsil consistently up to day 20 PI. The most remarkable clinical signs and virus detection appeared on day 1 PI. Data indicated that the number of infected chickens and viral RNA detection from tissues was reduced with increasing antibody titer on day 20 PI. The results demonstrated that the IRFIBV32 virus has wide tissue distribution for respiratory, urogenital, and digestive systems.

scholarly journals Effect of infectious bronchitis and Newcastle disease vaccines on experimental avian influenza infection (H9N2) in broiler chickens

2021 ◽  
Vol 24 (4) ◽  
pp. 574-585
Author(s):  
R. Amanollahi ◽  
K. Asasi ◽  
B. Abdi-Hachesoo ◽  
N. Ahmadi ◽  
A. Mohammadi
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease ◽  
Broiler Chickens ◽  
Clinical Signs ◽  
Respiratory Pathogen ◽  
Economic Losses ◽  
Control Group ◽  
Virus Shedding ◽  
Live Vaccines ◽  
Infectious Bronchitis

Despite the fact that H9N2 avian influenza virus (AIV) is considered a low-pathogenic agent, frequent outbreaks of this subtype have caused high mortality and economic losses in poultry farms around the world including Iran. Coinfection with a respiratory pathogen or environmental factors may explain the exacerbation of H9N2 AIV infection. In this study, the role of infectious bronchitis (IB) vaccines (H120 and 4/91) and Newcastle disease (ND) vaccines (B1 and LaSota) on experimental H9N2 AIV infection was investigated in 180 broiler chickens allotted into 6 groups (n=30). At the age of 18 days, groups 3 and 4 received H120 and 4/91 infectious bronchitis live vaccines (IBLVs) and groups 5 and 6 received B1 and LaSota Newcastle disease live vaccines (NDLVs), respectively. At the age of 20 days, all birds in the experimental groups except the negative control group (group 1), were inoculated intra-nasally with H9N2 AIV. After the inoculation, clinical signs, gross and microscopic lesions, and viral detection were examined. The results of this study revealed that clinical signs, gross and microscopic lesions were more severe in the AIV challenged groups which had been previously vaccinated with IB vaccines. In addition, AI viral RNA from tracheal and faecal samples in IB vaccinated birds were recovered at a higher rate. Moreover, in the 4/91 IB vaccinated group, the AI virus shedding period was longer than the other challenged groups. In conclusion, infectious bronchitis live vaccines (IBLVs) exacerbated the H9N2 AIV infection; also, 4/91 IBLV extended AI virus shedding period and increased the recovery rate of AI virus from feaces. However, the coinfection of Newcastle disease live vaccines (NDLVs) had no considerable adverse effects on AIV infection in broiler chickens.

Intestinal Tropism of an Infectious Bronchitis Virus Isolate Not Explained by Spike Protein Binding Specificity

2019 ◽  
Vol 64 (1) ◽  
pp. 23 ◽  
Author(s):  
Farjana Saiada ◽  
Rodrigo A. Gallardo ◽  
H. L. Shivaprasad ◽  
Charles Corsiglia ◽  
Vicky L. Van Santen
Keyword(s):  
Protein Binding ◽  
Infectious Bronchitis Virus ◽  
Virus Isolate ◽  
Binding Specificity ◽  
Spike Protein ◽  
Infectious Bronchitis ◽  
Infectious Bronchitis Virus Isolate

scholarly journals First Complete Genome Sequence of Currently Circulating Infectious Bronchitis Virus Strain DMV/1639 of the GI-17 Lineage

2019 ◽  
Vol 8 (34) ◽  
Author(s):  
Iryna V. Goraichuk ◽  
Arun B. Kulkarni ◽  
Dawn Williams-Coplin ◽  
David L. Suarez ◽  
Claudio L. Afonso
Keyword(s):  
Genome Sequence ◽  
Virus Strain ◽  
Infectious Bronchitis Virus ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Broiler Chickens ◽  
Economic Losses ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis Virus Strain ◽  
Infectious Bronchitis

Avian infectious bronchitis virus is the causative agent of a highly contagious disease that results in severe economic losses to the poultry industry worldwide. Here, we report the first coding-complete genome sequence of strain DMV/1639 of the GI-17 lineage, isolated from broiler chickens in Georgia in 2019.

scholarly journals Experimental study on histopathological changes and tissue tropism of Iranian infectious bronchitis serotype 793/B-like virus in SPF chickens

2013 ◽  
Vol 84 (1) ◽  
Author(s):  
Peyman Bijanzad ◽  
Reza Momayez ◽  
Mohammad H. Bozorgmehrifard ◽  
Mohammad H. Hablolvarid ◽  
Seyed A. Pourbakhsh
Keyword(s):  
Infectious Bronchitis Virus ◽  
Bursa Of Fabricius ◽  
Control Group ◽  
Lymphoid Tissues ◽  
Tissue Tropism ◽  
Post Inoculation ◽  
Avian Infectious Bronchitis Virus ◽  
Histopathological Changes ◽  
Infectious Bronchitis ◽  
Histopathological Lesions

Avian infectious bronchitis virus (IBV) is prevalent in all countries with intensive poultry flocks. This disease is characterised primarily by respiratory signs, but some IBV strains may also infect other organs such as the intestinal and urogenital tracts. The aim of this study was to characterise the histopathological lesions and tissue tropism of Iranian isolate IR/773/2001(793/B) of avian infectious bronchitis virus in different organs of experimentally infected SPF chickens. Forty-two one-day-old, specific pathogen-free (SPF) chicks were divided randomly into two groups (21 chicks to each group). At the age of 12 days, one group was inoculated intra-ocularly with 103 EID50 of the 793/B isolate, and the other was kept as the control group. Tissue samples were collected at 2, 4, 6, 8, 10 and 12 days post-inoculation (PI). The IBV virus was detected in the caecal tonsils and cloaca from the 2nd to the 12th day PI. The virus was also detected in the kidneys from days 4–10 PI and in the bursa of Fabricius from days 4–12 PI. The virus was detected in the trachea, lungs and thymus. The most obvious histopathological lesions were found in the trachea, kidney, lungs and bursa of Fabricius. Amongst the lymphoid tissues, histopathological changes were found most frequently in the bursa of Fabricius. The results of this study indicated that the 793/B serotype of IBV is unlikely to cause mortality, severe clinical signs or gross lesions in infected chickens, but its replication in some tissues including the bursa of Fabricius could render birds susceptible to other micro-organisms.

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