scholarly journals Various levels and forms of dietary α-lipoic acid in broiler chickens: Impact on blood biochemistry, stress response, liver enzymes, and antibody titers

2015 ◽  
Vol 94 (2) ◽  
pp. 226-231 ◽  
Author(s):  
D.W. Kim ◽  
M.M.H. Mushtaq ◽  
R. Parvin ◽  
H.K. Kang ◽  
J.H. Kim ◽  
...  
Keyword(s):  
Stress Response ◽  
Lipoic Acid ◽  
Broiler Chickens ◽  
Liver Enzymes ◽  
Blood Biochemistry ◽  
Antibody Titers

scholarly journals Effects of phytobiotic feed additives on growth traits, blood biochemistry, and meat characteristics of broiler chickens exposed to Salmonella typhimurium

2020 ◽  
Vol 99 (11) ◽  
pp. 5744-5751
Author(s):  
Mashael R. Aljumaah ◽  
Gamaleldin M. Suliman ◽  
Abdulaziz A. Abdullatif ◽  
Alaeldein M. Abudabos
Keyword(s):  
Salmonella Typhimurium ◽  
Broiler Chickens ◽  
Growth Traits ◽  
Blood Biochemistry ◽  
Feed Additives

scholarly journals The effect of microbial phytase and myo-inositol on performance and blood biochemistry of broiler chickens fed wheat/corn-based diets

2013 ◽  
Vol 92 (8) ◽  
pp. 2124-2134 ◽  
Author(s):  
A.J. Cowieson ◽  
A. Ptak ◽  
P. Maćkowiak ◽  
M. Sassek ◽  
E. Pruszyńska-Oszmałek ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Blood Biochemistry ◽  
Microbial Phytase

scholarly journals Immunization of Broiler Chickens against Clostridium perfringens-Induced Necrotic Enteritis

2007 ◽  
Vol 14 (9) ◽  
pp. 1070-1077 ◽  
Author(s):  
R. R. Kulkarni ◽  
V. R. Parreira ◽  
S. Sharif ◽  
J. F. Prescott
Keyword(s):  
Clostridium Perfringens ◽  
Broiler Chickens ◽  
Hypothetical Protein ◽  
Oral Infection ◽  
Secreted Proteins ◽  
Enzyme Linked Immunosorbent Assay ◽  
Necrotic Enteritis ◽  
Alpha Toxin ◽  
Genes Encoding ◽  
Antibody Titers

ABSTRACT Necrotic enteritis (NE) in broiler chickens is caused by Clostridium perfringens. Currently, no vaccine against NE is available and immunity to NE is not well characterized. Our previous studies showed that immunity to NE followed oral infection by virulent rather than avirulent C. perfringens strains and identified immunogenic secreted proteins apparently uniquely produced by virulent C. perfringens isolates. These proteins were alpha-toxin, glyceraldehyde-3-phosphate dehydrogenase, pyruvate:ferredoxin oxidoreductase (PFOR), fructose 1,6-biphosphate aldolase, and a hypothetical protein (HP). The current study investigated the role of each of these proteins in conferring protection to broiler chickens against oral infection challenges of different severities with virulent C. perfringens. The genes encoding these proteins were cloned and purified as histidine-tagged recombinant proteins from Escherichia coli and were used to immunize broiler chickens intramuscularly. Serum and intestinal antibody responses were assessed by enzyme-linked immunosorbent assay. All proteins significantly protected broiler chickens against a relatively mild challenge. In addition, immunization with alpha-toxin, HP, and PFOR also offered significant protection against a more severe challenge. When the birds were primed with alpha-toxoid and boosted with active toxin, birds immunized with alpha-toxin were provided with the greatest protection against a severe challenge. The serum and intestinal washings from protected birds had high antigen-specific antibody titers. Thus, we conclude that there are certain secreted proteins, in addition to alpha-toxin, that are involved in immunity to NE in broiler chickens.

scholarly journals Evaluation of Genotoxic And Biochemical Stress Response To Mercuric Chloride In Erythrocytes of Freshwater Fish Channa Punctatus

2021 ◽  
Author(s):  
Harmanpreet Kaur Gill ◽  
Kapil Paul ◽  
Anish Dua
Keyword(s):  
Stress Response ◽  
Comet Assay ◽  
Mercuric Chloride ◽  
Biochemical Analysis ◽  
Micronucleus Assay ◽  
Blood Biochemistry ◽  
Channa Punctatus ◽  
Genotoxic Effects ◽  
Toxicity Assay ◽  
Blood Biochemical

Abstract Experiments were conducted to evaluate health of fish, Channa punctatus inhabiting mercuric chloride. Acute toxicity bioassays were performed to calculate 96h LC50 value and it was found to be 1.38mg/L. Chronic toxicity assay to investigate genotoxic effects on the erythrocytes of fish by comet assay and micronucleus assay along with alterations in blood biochemistry were evaluated. Results showed concentration and duration dependent significant DNA damage as observed by comet assay and micronucleus assay. The frequency of nuclear aberrations along with appearance of micronuclei were observed after 30 and 60 days of exposure. The blood biochemistry was studied by recording changes in levels of various biochemical parameters in blood serum and results showed the significant (p < 0.05) variations among levels of biochemical constituents such as Glucose, Lipids, Proteins, Bilirubin, Urea, Creatinine, Cholesterol, ALP, Albumin, SGOT, SGPT and Total Glycerides. The resultsindicated the stress response of specimen towards toxicant. The present study highly recommends the use of genotoxicity and blood biochemical analysis as the useful biomarker to assess toxicity in the aquatic water and hence to safeguards the surrounding ecosystem.

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