scholarly journals Liver enzymes in White Leghorns selected for the sheep red blood cell immune response

2012 ◽  
Vol 91 (2) ◽  
pp. 322-326 ◽  
Author(s):  
S. Blevins ◽  
P.B. Siegel ◽  
D.J. Blodgett ◽  
M. Ehrich ◽  
R.M. Lewis
Keyword(s):  
Immune Response ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Liver Enzymes ◽  
Cell Immune Response ◽  
White Leghorns

scholarly journals Blood single cell immune profiling reveals the interferon-MAPK pathway mediated adaptive immune response for COVID-19

2020 ◽  
Author(s):  
Lulin Huang ◽  
Yi Shi ◽  
Bo Gong ◽  
Li Jiang ◽  
Xiaoqi Liu ◽  
...  
Keyword(s):  
Immune Response ◽  
Blood Cell ◽  
Single Cell ◽  
Defense Mechanism ◽  
Mapk Pathway ◽  
Adaptive Immune Response ◽  
Cell Immune Response ◽  
Marker Genes ◽  
Response Profiles ◽  
Immune Profiling

AbstractThe coronavirus disease 2019 (COVID-19) outbreak is an ongoing global health emergence, but the pathogenesis remains unclear. We revealed blood cell immune response profiles using 5’ mRNA, TCR and BCR V(D)J transcriptome analysis with single-cell resolution. Data from 134,620 PBMCs and 83,387 TCR and 12,601 BCR clones was obtained, and 56 blood cell subtypes and 23 new cell marker genes were identified from 16 participants. The number of specific subtypes of immune cells changed significantly when compared patients with controls. Activation of the interferon-MAPK pathway is the major defense mechanism, but MAPK transcription signaling is inhibited in cured patients. TCR and BCR V(D)J recombination is highly diverse in generating different antibodies against SARS-CoV-2. Therefore, the interferon-MAPK pathway and TCR-and BCR-produced antibodies play important roles in the COVID-19 immune response. Immune deficiency or immune over-response may result in the condition of patients with COVID-19 becoming critical or severe.

Immune Response of the Duck to Particulate (Red Blood Cell) Antigens

1981 ◽  
Vol 25 (2) ◽  
pp. 353 ◽  
Author(s):  
T. E. Toth ◽  
N. E. Norcross
Keyword(s):  
Immune Response ◽  
Blood Cell ◽  
Red Blood Cell

scholarly journals Expression and characterization of recombinant anti-Rh(D) antibodies on filamentous phage: a model system for isolating human red blood cell antibodies by repertoire cloning

Blood ◽  
1994 ◽  
Vol 83 (8) ◽  
pp. 2334-2344 ◽  
Author(s):  
DL Siegel ◽  
LE Silberstein
Keyword(s):  
Immune Response ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Expression Vectors ◽  
Filamentous Phage ◽  
Soluble Antigen ◽  
Fab Fragments ◽  
Human Immune Response ◽  
Membrane Bound ◽  
Human Immune

Abstract The production of human anti-red blood cell (RBC) Igs in vitro from immunized individuals would greatly facilitate the genetic analysis of the human immune response to RBC antigens and also provide useful serologic reagents. Technical difficulties inherent in human B-cell immortalization have led to the development of molecular approaches that bypass the need for cell transformation. By cloning human Ig gene segments into bacterial expression vectors, libraries are created of filamentous phage particles displaying Fab fragments on their surfaces. Libraries have been screened with purified, soluble antigen and selected clones genetically manipulated in Escherichia coli to produce soluble Fab fragments. Our goal has been to adapt this technique to the study of RBC autoantibodies and alloantibodies that have specificities against unpurifiable membrane-bound antigens. To test the feasibility of this approach, two sets of phage were created, one set expressing a human anti-Rh(D) Ig and the other expressing a human antitetanus toxoid Ig. After verifying the presence of functional phage-displayed Fabs through biochemical, flow cytometric, and electron microscopic analyses, a model library was constructed comprising one anti-Rh(D)- expressing phage per 10(4) antitetanus toxoid-expressing phage. A method was developed for screening the library with intact Rh(D)- positive RBCs. After four rounds of panning, anti-Rh(D) specificity was enriched more than 10,000-fold to a final frequency of approximately 100%. Plasmid DNA derived from anti-Rh(D) phage was used to produce milligram quantities of soluble recombinant anti-Rh(D) Fabs purified by nitrogen cavitation and nickel-chelation affinity chromatography. The authenticity of the Fabs was confirmed by sodium dodecyl sulfate- polyacrylamide gel electrophoresis and immunoblotting, which showed bands with molecular weights of approximately 50 kD and 26 kD under nonreducing and reducing conditions, respectively. Binding of recombinant anti-Rh(D) Fabs to Rh(D)-positive RBCs was demonstrated by flow cytometry and by an agglutination assay. Our results suggest that repertoire cloning by cell-surface enrichment may have broad application to the study of the human immune response to erythroid antigens in addition to membrane-bound antigens present on other hematopoietic cells.

scholarly journals Correlated Responses to Bi-Directional Selection for Sheep Red Blood Cell Titer in White Leghorns

2012 ◽  
Vol 11 (16) ◽  
pp. 2879-2884 ◽  
Author(s):  
Qing Zhu ◽  
Yao-Dong Hu ◽  
Jie Wen ◽  
Mai-Qing Zheng ◽  
Ran-Ran Liu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Directional Selection ◽  
Correlated Responses ◽  
White Leghorns ◽  
Selection For
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